N Cadherin Antibody - #AF4039

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产品: N Cadherin 抗体
货号: AF4039
描述: Rabbit polyclonal antibody to N Cadherin
应用: WB IHC IF/ICC
文献验证: WB, IHC, IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
蛋白号: P19022
RRID: AB_2835344

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IHC 1:50-200, IF/ICC 1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
N Cadherin Antibody detects endogenous levels of total N Cadherin.
RRID:
AB_2835344
引用格式: Affinity Biosciences Cat# AF4039, RRID:AB_2835344.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CADH2_HUMAN; Cadherin 2; Cadherin 2 N cadherin neuronal; Cadherin 2 type 1; Cadherin 2 type 1 N cadherin neuronal; Cadherin 2, type 1, N-cadherin (neuronal); Cadherin-2; Cadherin2; Calcium dependent adhesion protein neuronal; CD325; CD325 antigen; CDH2; CDHN; CDw325; CDw325 antigen; N cadherin 1; N-cadherin; NCAD; Neural cadherin; OTTHUMP00000066304; OTTHUMP00000067378;

抗原和靶标

免疫原:

A synthesized peptide derived from human N Cadherin, corresponding to a region within C-terminal amino acids.

基因/基因ID:
CDH2(1000)
描述:
Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density

研究领域

· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs).   (View pathway)

· Human Diseases > Cardiovascular diseases > Arrhythmogenic right ventricular cardiomyopathy (ARVC).

文献引用

1). METTL13 Mediates the Translation of Snail in Head and Neck Squamous Cell Carcinoma. International Journal of Oral Science, 2020 (PubMed: 34381012) [IF=10.8]
2). Methyltransferase like 13 mediates the translation of Snail in head and neck squamous cell carcinoma. International journal of oral science, 2021 (PubMed: 34381012) [IF=10.8]
3). Roles of gut microbiome-associated metabolites in pulmonary fibrosis by integrated analysis. NPJ biofilms and microbiomes, 2024 (PubMed: 39702426) [IF=9.2]
4). METTL3 promotes prostatic hyperplasia by regulating PTEN expression in an m6A-YTHDF2-dependent manner. Cell death & disease, 2022 (PubMed: 35985997) [IF=8.1]

Application: WB    Species: human    Sample: BPH-1 cells

Fig. 3: Effects of METTL3 knockdown on proliferation, apoptosis, and EMT of TGF-β-treated BPH-1 cells. METTL3 knockdown was achieved in TGF-β-treated BPH-1 cells by transducing Lv-sh-METTL3 and then examined for A, B METTL3 expression using qRT-PCR (A) and Immunoblotting (B); C m6A status in RNA using an m6A RNA Methylation Assay Kit; D cell viability using MTT assay; E DNA synthesis using EdU assay; Scale bar = 20 μm; F the protein levels of E-cadherin, N-cadherin, vimentin using Immunoblotting; G cell apoptosis using flow cytometry; H the protein levels of Bcl-2, Bax, Caspase 9, cleaved caspase 9, Caspase 3, cleaved caspase 3, and cleaved PARP-1 using Immunoblotting. **P 
5). Huaier polysaccharides suppress triple-negative breast cancer metastasis and epithelial-mesenchymal transition by inducing autophagic degradation of Snail. Cell and Bioscience, 2021 (PubMed: 34481526) [IF=7.5]

Application: WB    Species: human    Sample: breast cancer cells

Fig. 1| PS-T inhibits invasion, migration and EMT in breast cancer cells in vitro and in vivo. d 4 T-1 and MDA-MB-231 cells are treated with 5 μg/mL PS-T for 24 h. The levels of each EMT marker are quantifed using the NIH ImageJ software. (mean ± SD, *P < 0.05 and **P < 0.01)

Application: WB    Species: Human    Sample: breast cancer cells

Fig. 1 PS-T inhibits invasion, migration and EMT in breast cancer cells in vitro and in vivo. a The invasiveness of 4 T-1 and MDA-MB-231 cells after treatment with PS-T at 5 μg/mL for 12 h is evaluated using Transwell invasion assays at 24 h (mean  ±  SD, ***P  <  0.001). b Migratory ability of 4 T-1 and MDA-MB-231 cells after treatment with PS-T at different concentrations is evaluated using scratch assays at different time points (mean  ±  SD, **P  <  0.01, ***P  <  0.001). c Mice are treated with normal saline (control), 25 μg/g or 100 μg/g PS-T by oral gavage every other day for 21 days (mean  ±  SD, *P  <  0.05, **P  <  0.01, scale bars: 100 μm). d 4 T-1 and MDA-MB-231 cells are treated with 5 μg/mL PS-T for 24 h. The levels of each EMT marker are quantified using the NIH ImageJ software. (mean  ±  SD, *P  <  0.05 and **P  <  0.01)
6). TRIP13 interference inhibits the proliferation and metastasis of thyroid cancer cells through regulating TTC5/p53 pathway and epithelial-mesenchymal transition related genes expression. BIOMEDICINE & PHARMACOTHERAPY, 2019 (PubMed: 31648166) [IF=6.9]
7). d-Borneol enhances cisplatin sensitivity via autophagy dependent EMT signaling and NCOA4-mediated ferritinophagy. PHYTOMEDICINE, 2022 (PubMed: 36030746) [IF=6.7]
8). Molecular mechanism of albumin in suppressing invasion and metastasis of hepatocellular carcinoma. LIVER INTERNATIONAL, 2022 (PubMed: 34854209) [IF=6.0]

Application: WB    Species: Human    Sample: HepG2 and Huh7 cells

FIGURE 7 A, Representative images of the western blot results for uPAR, MMP2 and MMP9 in ALB knockdown HepG2 and Huh7 cells; B, Zymography analysis illustrates MMP2 and MMP9 activity in ALB knockdown HepG2 and Huh7 cells; C, Quantitative analysis results and representative images of the western blot results for the EMT‐associated markers, E‐cadherin, N‐cadherin, vimentin, Snail and Twist by western blot in ALB knockdown HepG2 and Huh7 cells; D, Quantification shows a significantly higher uPAR in HCC group with ALB <3.5 g/dL compared to ALB ≥3.5 g/dL (*P < .05); E, Scatterplot showing the correlation between plasma levels of ALB and uPAR. The vertical position represents the expression levels of uPAR (lg pg/mL)
9). Elevated expression of HIGD1A drives hepatocellular carcinoma progression by regulating polyamine metabolism through c-Myc-ODC1 nexus. Cancer & metabolism, 2024 (PubMed: 38395945) [IF=5.9]

Application: WB    Species: human    Sample: L02, HepG2, Huh7, and MHCC97H cells

Fig. 3 HIGD1A knockdown inhibits the migration and invasion of HCC cells. A Cell migration ability of L02, HepG2, Huh7, and MHCC97H cells infected with shCtrl or shHIGD1A lentivirus was analyzed by transwell assay. Representative images of the transwell assay (left) and quantitative data of migrated cells (right) are shown. B Cell invasion ability of L02, HepG2, Huh7, and MHCC97H cells infected with shCtrl or shHIGD1A lentivirus as analyzed by transwell assay. Representative images of the transwell assay (left) and quantitative data of migrated cells (right) are shown. C Expression of EMT markers E-cadherin and N-cadherin in L02, HepG2, Huh7, and MHCC97H cells infected with shCtrl or shHIGD1A lentivirus as examined by western blotting. Results shown are mean ± SEM. *P
10). Polydopamine/carboxylic graphene oxide-composited polypyrrole films for promoting adhesion and alignment of Schwann cells. Colloids and surfaces-B, Biointerfaces, 2020 (PubMed: 32203860) [IF=5.4]

Application: WB    Species:    Sample: RSCs

Fig. 3.| Cell images of immunofluorescence staining for (a) N-cadherin expression, phalloidin, (b) GFAP, (c) S100 and DAPI, and scale bar is 50 μm. (d) WB assay for Vinculin, Ncadherin, P75 and GFAP proteins and (e) their semi-quantitative comparison. & shows p＜0.05, compared with control; # shows p＜0.05, compared with PPy-PLLA; Δ shows p＜0.05, compared with CGO/PPy-PLLA.

Application: IF/ICC    Species:    Sample: RSCs

Fig. 3.| Cell images of immunofluorescence staining for (a) N-cadherin expression, phalloidin, (b) GFAP, (c) S100 and DAPI, and scale bar is 50 μm. (d) WB assay for Vinculin, Ncadherin, P75 and GFAP proteins and (e) their semi-quantitative comparison. & shows p＜0.05, compared with control; # shows p＜0.05, compared with PPy-PLLA; Δ shows p＜0.05, compared with CGO/PPy-PLLA.
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