产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
展开/折叠
6 Phosphofructokinase Muscle Type; 6-phosphofructokinase; 6-phosphofructokinase muscle type; EC 2.7.1.1; EC 2.7.1.11; GSD7; K6PF_HUMAN; MGC8699; muscle type; PFK, muscle type; PFK-A; PFKA; PFKL; PFKM; PFKP; PFKX; Phosphofructo 1 Kinase Isozyme A; Phosphofructo-1-kinase isozyme A; Phosphofructokinase 1; Phosphofructokinase M; Phosphofructokinase, muscle; Phosphofructokinase, muscle type; Phosphofructokinase, polypeptide X; Phosphofructokinase-M; Phosphohexokinase;
抗原和靶标
- P08237 PFKAM_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTHEEHHAAKTLGIGKAIAVLTSGGDAQGMNAAVRAVVRVGIFTGARVFFVHEGYQGLVDGGDHIKEATWESVSMMLQLGGTVIGSARCKDFREREGRLRAAYNLVKRGITNLCVIGGDGSLTGADTFRSEWSDLLSDLQKAGKITDEEATKSSYLNIVGLVGSIDNDFCGTDMTIGTDSALHRIMEIVDAITTTAQSHQRTFVLEVMGRHCGYLALVTSLSCGADWVFIPECPPDDDWEEHLCRRLSETRTRGSRLNIIIVAEGAIDKNGKPITSEDIKNLVVKRLGYDTRVTVLGHVQRGGTPSAFDRILGSRMGVEAVMALLEGTPDTPACVVSLSGNQAVRLPLMECVQVTKDVTKAMDEKKFDEALKLRGRSFMNNWEVYKLLAHVRPPVSKSGSHTVAVMNVGAPAAGMNAAVRSTVRIGLIQGNRVLVVHDGFEGLAKGQIEEAGWSYVGGWTGQGGSKLGTKRTLPKKSFEQISANITKFNIQGLVIIGGFEAYTGGLELMEGRKQFDELCIPFVVIPATVSNNVPGSDFSVGADTALNTICTTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFTIRDLQANVEHLVQKMKTTVKRGLVLRNEKCNENYTTDFIFNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAKAMNWMSGKIKESYRNGRIFANTPDSGCVLGMRKRALVFQPVAELKDQTDFEHRIPKEQWWLKLRPILKILAKYEIDLDTSDHAHLEHITRKRSGEAAV
翻译修饰 - P08237 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T2 | Phosphorylation | Uniprot | |
| K10 | Acetylation | Uniprot | |
| K10 | Ubiquitination | Uniprot | |
| K16 | Ubiquitination | Uniprot | |
| S74 | Phosphorylation | Uniprot | |
| T82 | Phosphorylation | Uniprot | |
| Y103 | Phosphorylation | Uniprot | |
| S130 | Phosphorylation | Uniprot | |
| S133 | Phosphorylation | Uniprot | |
| S137 | Phosphorylation | Uniprot | |
| K141 | Ubiquitination | Uniprot | |
| K144 | Ubiquitination | Uniprot | |
| S198 | Phosphorylation | Uniprot | |
| T202 | Phosphorylation | Uniprot | |
| K272 | Ubiquitination | Uniprot | |
| K280 | Ubiquitination | Uniprot | |
| R301 | Methylation | Uniprot | |
| T304 | Phosphorylation | Uniprot | |
| R310 | Methylation | Uniprot | |
| R315 | Methylation | Uniprot | |
| T328 | Phosphorylation | Uniprot | |
| T331 | Phosphorylation | Uniprot | |
| T359 | Phosphorylation | Uniprot | |
| K360 | Acetylation | Uniprot | |
| K360 | Ubiquitination | Uniprot | |
| K366 | Acetylation | Uniprot | |
| K366 | Ubiquitination | Uniprot | |
| K372 | Methylation | Uniprot | |
| K372 | Ubiquitination | Uniprot | |
| S377 | Phosphorylation | Uniprot | |
| S398 | Phosphorylation | Uniprot | |
| S400 | Phosphorylation | Uniprot | |
| T402 | Phosphorylation | Uniprot | |
| K445 | Ubiquitination | Uniprot | |
| S454 | Phosphorylation | Uniprot | |
| K466 | Ubiquitination | Uniprot | |
| T472 | Phosphorylation | Uniprot | |
| K476 | Ubiquitination | Uniprot | |
| S477 | Phosphorylation | Uniprot | |
| K557 | Ubiquitination | Uniprot | |
| K564 | Acetylation | Uniprot | |
| Y576 | Phosphorylation | Uniprot | |
| K615 | Ubiquitination | Uniprot | |
| K656 | Ubiquitination | Uniprot | |
| S667 | Phosphorylation | Uniprot | |
| R673 | Methylation | Uniprot | |
| T677 | Phosphorylation | Uniprot | |
| K678 | Ubiquitination | Uniprot | |
| K682 | Ubiquitination | Uniprot | |
| K727 | Acetylation | Uniprot | |
| K727 | Ubiquitination | Uniprot | |
| K738 | Acetylation | Uniprot | |
| K738 | Ubiquitination | Uniprot | |
| K754 | Ubiquitination | Uniprot | |
| Y755 | Phosphorylation | Uniprot | |
| S762 | Phosphorylation | Uniprot | |
| K773 | Ubiquitination | Uniprot | |
| S775 | Phosphorylation | Uniprot |
研究背景
Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.
GlcNAcylation decreases enzyme activity.
Cytoplasm.
Homo- and heterotetramers (By similarity). Phosphofructokinase (PFK) enzyme functions as a tetramer composed of different combinations of 3 types of subunits, called PFKM (where M stands for Muscle), PFKL (Liver) and PFKP (Platelet). The composition of the PFK tetramer differs according to the tissue type it is present in. In muscles, it is composed of 4 PFKM subunits (also called M4). In the liver, the predominant form is a tetramer of PFKL subunits (L4). In erythrocytes, both PFKM and PFKL subunits randomly tetramerize to form M4, L4 and other combinations (ML3, M2L2, M3L). The kinetic and regulatory properties of the tetrameric enzyme are dependent on the subunit composition, hence can vary across tissues (Probable). Interacts (via C-terminus) with HK1 (via N-terminal spermatogenic cell-specific region) (By similarity).
Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade 'E' sub-subfamily.
研究领域
· Environmental Information Processing > Signal transduction > AMPK signaling pathway. (View pathway)
· Genetic Information Processing > Folding, sorting and degradation > RNA degradation.
· Human Diseases > Cancers: Overview > Central carbon metabolism in cancer. (View pathway)
· Metabolism > Carbohydrate metabolism > Glycolysis / Gluconeogenesis.
· Metabolism > Carbohydrate metabolism > Pentose phosphate pathway.
· Metabolism > Carbohydrate metabolism > Fructose and mannose metabolism.
· Metabolism > Carbohydrate metabolism > Galactose metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
· Metabolism > Global and overview maps > Carbon metabolism.
· Metabolism > Global and overview maps > Biosynthesis of amino acids.
限制条款
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