LC3A Antibody - #AF4007

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产品: LC3A 抗体
货号: AF4007
描述: Rabbit polyclonal antibody to LC3A
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Chicken, Xenopus
蛋白号: Q9H492
RRID: AB_2835334

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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说明书
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:1000-1:5000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
MAP1LC3A Antibody detects endogenous levels of total MAP1LC3A.
RRID:
AB_2835334
引用格式: Affinity Biosciences Cat# AF4007, RRID:AB_2835334.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

ATG8E; Autophagy-related protein LC3 A; Autophagy-related ubiquitin-like modifier LC3 A; LC3; LC3A; MAP1 light chain 3 like protein 1; MAP1 light chain 3-like protein 1; MAP1A/1B light chain 3 A; MAP1A/MAP1B LC3 A; MAP1A/MAP1B light chain 3 A; MAP1ALC3; MAP1BLC3; Map1lc3a; Microtubule associated proteins 1A/1B light chain 3; Microtubule-associated protein 1 light chain 3 alpha; Microtubule-associated proteins 1A and 1B, light chain 3; Microtubule-associated proteins 1A/1B light chain 3A; MLP3A_HUMAN;

抗原和靶标

免疫原:

A synthesized peptide derived from human MAP1LC3A, corresponding to a region within the internal amino acids.

基因/基因ID:
MAP1LC3A(84557)
描述:
Probably involved in formation of autophagosomal vacuoles (autophagosomes).

研究领域

· Cellular Processes > Cell growth and death > Ferroptosis.   (View pathway)

文献引用

1). Zinc ions facilitate metabolic bioenergetic recovery post spinal cord injury by activating microglial mitophagy through the STAT3-FOXO3a-SOD2 pathway. Free radical biology & medicine, 2024 (PubMed: 39613048) [IF=7.1]
2). Shufeng Jiedu capsule ameliorates olfactory dysfunction via the AMPK/mTOR autophagy pathway in a mouse model of allergic rhinitis. PHYTOMEDICINE, 2022 (PubMed: 36116201) [IF=6.7]
3). Network pharmacology, molecular docking, and in vitro experimental verification of the mechanism of Guanxining in treating diabetic atherosclerosis. Journal of ethnopharmacology, 2024 (PubMed: 38290612) [IF=4.8]
4). CH25H Promotes Autophagy and Regulates the Malignant Progression of Laryngeal Squamous Cell Carcinoma Through the PI3K-AKT Pathway. Cancer medicine, 2024 (PubMed: 39428922) [IF=2.9]

Application: WB    Species: Human    Sample: LSCC Cells

FIGURE 5. CH25H inhibited the proliferation of LSCC cells (A) After overexpressing CH25H in TU‐686 and knocking down CH25H in AMC‐HN‐8, the expression of CH25H was detected; (B) After TU‐686 were overexpressed with CH25H and AMC‐HN‐8 were knocked down with CH25H, the cell viability was detected; (C) After TU‐686 were overexpressed with CH25H and AMC‐HN‐8 were knocked down with CH25H, the cell colony formation was detected; (D) The cell proliferation of each group was displayed by the bar chart; (E) After TU‐686 were overexpressed with CH25H and AMC‐HN‐8 were knocked down with CH25H, the cell apoptosis was detected; (F) The apoptosis level of each group was displayed by the bar chart; (G) After TU‐686 were overexpressed with CH25H and AMC‐HN‐8 were knocked down with CH25H, the migration was detected; (H) After TU‐686 were overexpressed with CH25H and AMC‐HN‐8 were knocked down with CH25H, the invasion was detected; (I) After overexpressing CH25H in TU‐686 and knocking down CH25H in AMC‐HN‐8, the expression of autophagy‐related proteins was detected by WB (**p 
5). CircPAN3 contributes to drug resistance in acute myeloid leukemia through regulation of autophagy. LEUKEMIA RESEARCH, 2019 (PubMed: 31401408) [IF=2.1]

Application: WB    Species: human    Sample: THP-1, THP-1/ADM, K562 and K562/ADM cells

Fig. 3. |The levels of autophagy markers in drug-sensitive and -resistant AML cells. (A) Autophagy related proteins LC3-II/LC3-I, Beclin-1, and P62 were detected and quantified by Western blot in THP-1, THP-1/ADM, K562 and K562/ADM cells.

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