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![HIF1A Antibody - Figure 5: BHD inhibited protein and mRNA expression of pyruvate kinase M2 (PKM2) and hypoxia-inducible factor-1 alpha (HIF-1α) as well as its target genes (GLUT1, PDK1, lactate dehydrogenase A [LDHA]) in aortas after high fat diet treatment.](http://img.affbiotech.cn/uploads/202409/9639a09a44621edcf691119009aee434.png "Figure 5: BHD inhibited protein and mRNA expression of pyruvate kinase M2 (PKM2) and hypoxia-inducible factor-1 alpha (HIF-1α) as well as its target genes (GLUT1, PDK1, lactate dehydrogenase A [LDHA]) in aortas after high fat diet treatment. (a) Protein Levels of PKM2 were measured by Western blot analysis, and quantitative analysis was performed on the corresponding bands (n = 8–9). (b) Protein Levels of HIF-1α was measured by WB, and quantitative analysis was performed on the corresponding bands (n = 9). (c) The mRNA levels of GLUTA, (d) PDK1, and (e) LDHA in aortas (n = 6) were measured by qRT-PCR. Means ± standard error of mean. *P < 0.05, **P < 0.01, ***P < 0.001 versus Con; # P < 0.05, ## P < 0.01 versus Mod.")
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF1009, RRID:AB_2835328.
展开/折叠
ARNT interacting protein; ARNT-interacting protein; Basic helix loop helix PAS protein MOP1; Basic-helix-loop-helix-PAS protein MOP1; bHLHe78; Class E basic helix-loop-helix protein 78; HIF 1A; HIF 1alpha; HIF-1-alpha; HIF1 A; HIF1 Alpha; HIF1; HIF1-alpha; HIF1A; HIF1A_HUMAN; Hypoxia inducible factor 1 alpha; Hypoxia inducible factor 1 alpha isoform I.3; Hypoxia inducible factor 1 alpha subunit; Hypoxia inducible factor 1 alpha subunit basic helix loop helix transcription factor; Hypoxia inducible factor 1, alpha subunit (basic helix loop helix transcription factor); Hypoxia inducible factor1alpha; Hypoxia-inducible factor 1-alpha; Member of PAS protein 1; Member of PAS superfamily 1; Member of the PAS Superfamily 1; MOP 1; MOP1; PAS domain-containing protein 8; PASD 8; PASD8;
抗原和靶标
A synthesized peptide derived from human HIF1A, corresponding to a region within the internal amino acids.
研究领域
· Cellular Processes > Transport and catabolism > Autophagy - animal. (View pathway)
· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway. (View pathway)
· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)
· Human Diseases > Cancers: Overview > Proteoglycans in cancer.
· Human Diseases > Cancers: Specific types > Renal cell carcinoma. (View pathway)
· Human Diseases > Cancers: Overview > Central carbon metabolism in cancer. (View pathway)
· Human Diseases > Cancers: Overview > Choline metabolism in cancer. (View pathway)
· Organismal Systems > Immune system > Th17 cell differentiation. (View pathway)
· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway. (View pathway)
文献引用
Application: WB Species: Human Sample: K562 cells
Application: IHC Species: mouse Sample: lung
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