Cleaved-PARP (Asp214) Antibody - #AF7023

Fig. 4. Effect of edaravone and/or dexamethasone on smoke-induced cell apoptosis.

Fig. 4| Protective efect of baicalein on H­2O2 induced hepatotoxicity. L02 cells were pre-treated with 100 μM H­2O2 for 1 h, then co-incubated with 50 μM baicalein for 11 h. a Mitochondrial and apoptosis were stained with MitoTracker Red and TUNEL, respectively. b The percentage of cells underwent mitochondrial fssion and TUNEL positive cell. c The expression levels of apoptotic and autophagy related proteins were detected by western blotting and densitometry.

Fig. 4 Measured apoptosis and protein expression in C6 cells treated with (a) PBS, (b) Dextran, (c) Phycocyanin, (d) Cordycepin, (e) the Phycocyanin/Cordycepin mixture, or (f) Phy-Dex-Cord micelles for 24 h. (A) Trypan blue staining was used to observe cell morphology (Bar: 100 µm). (B) Apoptotic cells were detected with flow cytometry. Subcellular localization (C1) cleaved caspase-3 and (C2) cleaved PARP in C6 cells, as determined by Confocal laser scanning microscopy. (D1) Apoptosis-related protein, and (D2, D3) quantitative analysis of Bax, Bcl-2, p53, cleaved caspase-3, cleaved PARP, and PARP levels in C6 cells. Significant increase or decrease at labels (*) (p < 0.05), labels (**) (0.001< p < 0.01) and labels (***) p < 0.001, are identified in comparison with the Control group. (E) Cell counting determination of apoptotic rate with Trypan blue and statistical analysis of FCM results.