Cleaved-PARP (Asp214) Antibody - #AF7023

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产品: Cleaved-PARP (Asp214) 抗体
货号: AF7023
描述: Rabbit polyclonal antibody to Cleaved-PARP (Asp214)
应用: WB
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
蛋白号: P09874
RRID: AB_2835327

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 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Cleaved-PARP (Asp214) Antibody detects endogenous levels of fragment of activated PARP resulting from cleavage adjacent to Asp214.
RRID:
AB_2835327
引用格式: Affinity Biosciences Cat# AF7023, RRID:AB_2835327.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Stable for 14 months from date of receipt. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

ADP-ribosyltransferase diphtheria toxin-like 1; ADPRT 1; ADPRT; ADPRT1; APOPAIN; ARTD1; NAD(+) ADP-ribosyltransferase 1; PARP; PARP-1; PARP1; PARP1_HUMAN; Poly [ADP-ribose] polymerase 1; Poly ADP ribose polymerase 1; Poly[ADP-ribose] synthase 1; PPOL; SCA1;

抗原和靶标

免疫原:

The antiserum was produced against synthesized peptide derived from human PARP.

基因/基因ID:
PARP1(142)
描述:
Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks.

研究领域

· Cellular Processes > Cell growth and death > Apoptosis.   (View pathway)

· Cellular Processes > Cell growth and death > Necroptosis.   (View pathway)

· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway.   (View pathway)

· Genetic Information Processing > Replication and repair > Base excision repair.

文献引用

1). Cell-cycle arrest and mitochondria-dependent apoptosis induction in T-47D cells by the capsular polysaccharide from the marine bacterium Kangiella japonica KMM 3897. Carbohydrate Polymers, 2023 (PubMed: 37659798) [IF=10.7]
2). Structure and in vitro antiproliferative activity against breast cancer cells of the cell-wall polysaccharide from the marine bacterium Kangiella japonica KMM 3899T. Carbohydrate polymers, 2024 (PubMed: 38876721) [IF=10.7]
3). AKAP8L enhances the stemness and chemoresistance of gastric cancer cells by stabilizing SCD1 mRNA. Cell Death & Disease, 2022 (PubMed: 36522343) [IF=8.1]
4). Metformin modified chitosan as a multi-functional adjuvant to enhance cisplatin-based tumor chemotherapy efficacy. International Journal of Biological Macromolecules, 2023 (PubMed: 36283555) [IF=7.7]
5). Quercetin induces autophagy via FOXO1-dependent pathways and autophagy suppression enhances quercetin-induced apoptosis in PASMCs in hypoxia. FREE RADICAL BIOLOGY AND MEDICINE, 2017 (PubMed: 27979659) [IF=7.1]

Application: WB    Species: rat    Sample:

6). Apolipoprotein B100 acts as a tumor suppressor in ovarian cancer via lipid/ER stress axis-induced blockade of autophagy. Acta pharmacologica Sinica, 2025 (PubMed: 39880927) [IF=6.9]
7). Edaravone combined with dexamethasone exhibits synergic effects on attenuating smoke-induced inhalation lung injury in rats. Biomedicine & Pharmacotherapy, 2021 (PubMed: 34225014) [IF=6.9]

Application: WB    Species: Rat    Sample: lung tissues

Fig. 4. Effect of edaravone and/or dexamethasone on smoke-induced cell apoptosis.
8). Packaging cordycepin phycocyanin micelles for the inhibition of brain cancer. Journal of Materials Chemistry B, 2017 (PubMed: 32264358) [IF=6.1]

Application: WB    Species: Mouse    Sample: C6 cells

Fig. 4 Measured apoptosis and protein expression in C6 cells treated with (a) PBS, (b) Dextran, (c) Phycocyanin, (d) Cordycepin, (e) the Phycocyanin/Cordycepin mixture, or (f) Phy-Dex-Cord micelles for 24 h. (A) Trypan blue staining was used to observe cell morphology (Bar: 100 µm). (B) Apoptotic cells were detected with flow cytometry. Subcellular localization (C1) cleaved caspase-3 and (C2) cleaved PARP in C6 cells, as determined by Confocal laser scanning microscopy. (D1) Apoptosis-related protein, and (D2, D3) quantitative analysis of Bax, Bcl-2, p53, cleaved caspase-3, cleaved PARP, and PARP levels in C6 cells. Significant increase or decrease at labels (*) (p < 0.05), labels (**) (0.001< p < 0.01) and labels (***) p < 0.001, are identified in comparison with the Control group. (E) Cell counting determination of apoptotic rate with Trypan blue and statistical analysis of FCM results.
9). A Potent Protective Effect of Baicalein on Liver Injury by Regulating Mitochondria-Related Apoptosis. APOPTOSIS, 2020 (PubMed: 32409930) [IF=6.1]

Application: WB    Species: human    Sample: L02 cells

Fig. 4| Protective efect of baicalein on H­2O2 induced hepatotoxicity. L02 cells were pre-treated with 100 μM H­2O2 for 1 h, then co-incubated with 50 μM baicalein for 11 h. a Mitochondrial and apoptosis were stained with MitoTracker Red and TUNEL, respectively. b The percentage of cells underwent mitochondrial fssion and TUNEL positive cell. c The expression levels of apoptotic and autophagy related proteins were detected by western blotting and densitometry.
10). Carbamoylation at C-8 position of natural 3-arylcoumarin scaffold for the discovery of novel PARP-1 inhibitors with potent anticancer activity. European journal of medicinal chemistry, 2024 (PubMed: 39116535) [IF=6.0]
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