产品: Glutamine Synthetase 小鼠 单克隆 抗体
货号: BF8094
描述: Mouse monoclonal antibody to Glutamine Synthetase
应用: IHC
反应: Human
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 42 kDa; 42kD(Calculated).
蛋白号: P15104

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 50ul RMB¥ 1250 现货
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 200ul RMB¥ 3000 现货

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产品描述

来源:
Mouse
应用:
IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human
克隆:
Monoclonal [AFfirm8094]
特异性:
Glutamine Synthetase Antibody detects endogenous levels of total Glutamine Synthetase.
偶联:
Unconjugated.
纯化:
Affinity-chromatography.
保存:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

cell proliferation-inducing protein 59; GLNA; GLNA_HUMAN; GLNS; GLUL; Glutamate ammonia ligase; Glutamate decarboxylase; Glutamate--ammonia ligase; glutamine synthase; Glutamine synthetase; GS; PIG 43; PIG 59; PIG43; PIG59; Proliferation inducing protein 43;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
P15104 GLNA_HUMAN:

Expressed in endothelial cells.

序列:
MTTSASSHLNKGIKQVYMSLPQGEKVQAMYIWIDGTGEGLRCKTRTLDSEPKCVEELPEWNFDGSSTLQSEGSNSDMYLVPAAMFRDPFRKDPNKLVLCEVFKYNRRPAETNLRHTCKRIMDMVSNQHPWFGMEQEYTLMGTDGHPFGWPSNGFPGPQGPYYCGVGADRAYGRDIVEAHYRACLYAGVKIAGTNAEVMPAQWEFQIGPCEGISMGDHLWVARFILHRVCEDFGVIATFDPKPIPGNWNGAGCHTNFSTKAMREENGLKYIEEAIEKLSKRHQYHIRAYDPKGGLDNARRLTGFHETSNINDFSAGVANRSASIRIPRTVGQEKKGYFEDRRPSANCDPFSVTEALIRTCLLNETGDEPFQYKN

翻译修饰 - P15104 作为底物

Site PTM Type Enzyme
T2 Acetylation
K11 Acetylation
K11 Ubiquitination
K14 Acetylation
K14 Ubiquitination
Y17 Phosphorylation
S19 Phosphorylation
K25 Ubiquitination
K52 Ubiquitination
K91 Ubiquitination
K95 Ubiquitination
K103 Acetylation
K103 Ubiquitination
Y104 Phosphorylation
Y180 Phosphorylation
C183 S-Nitrosylation
Y185 Phosphorylation
K259 Ubiquitination
K268 Ubiquitination
Y269 Phosphorylation
K276 Ubiquitination
K291 Ubiquitination
T301 Phosphorylation
S320 Phosphorylation
S322 Phosphorylation
K334 Ubiquitination
Y336 Phosphorylation
S343 Phosphorylation
C359 S-Nitrosylation
Y371 Phosphorylation
K372 Acetylation
K372 Ubiquitination

研究背景

功能:

Glutamine synthetase that catalyzes the ATP-dependent conversion of glutamate and ammonia to glutamine. Its role depends on tissue localization: in the brain, it regulates the levels of toxic ammonia and converts neurotoxic glutamate to harmless glutamine, whereas in the liver, it is one of the enzymes responsible for the removal of ammonia (By similarity). Essential for proliferation of fetal skin fibroblasts. Independently of its glutamine synthetase activity, required for endothelial cell migration during vascular development: acts by regulating membrane localization and activation of the GTPase RHOJ, possibly by promoting RHOJ palmitoylation. May act as a palmitoyltransferase for RHOJ: able to autopalmitoylate and then transfer the palmitoyl group to RHOJ. Plays a role in ribosomal 40S subunit biogenesis.

翻译修饰:

Palmitoylated; undergoes autopalmitoylation.

Ubiquitinated by ZNRF1.

细胞定位:

Cytoplasm>Cytosol. Microsome. Mitochondrion. Cell membrane>Lipid-anchor.
Note: Mainly localizes in the cytosol, with a fraction associated with the cell membrane.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in endothelial cells.

亚基结构:

Decamer; composed of two pentamers. Interacts with PALMD (By similarity). Interacts with RHOJ.

蛋白家族:

Belongs to the glutamine synthetase family.

研究领域

· Cellular Processes > Cell growth and death > Necroptosis.   (View pathway)

· Metabolism > Amino acid metabolism > Arginine biosynthesis.

· Metabolism > Amino acid metabolism > Alanine, aspartate and glutamate metabolism.

· Metabolism > Carbohydrate metabolism > Glyoxylate and dicarboxylate metabolism.

· Metabolism > Energy metabolism > Nitrogen metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Biosynthesis of amino acids.

· Organismal Systems > Nervous system > Glutamatergic synapse.

· Organismal Systems > Nervous system > GABAergic synapse.

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