TIMP1 Antibody - #AF7007

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产品: TIMP1 抗体
货号: AF7007
描述: Rabbit polyclonal antibody to TIMP1
应用: WB IHC IF/ICC
文献验证: WB, IHC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Dog
蛋白号: P01033
RRID: AB_2835315

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
TIMP1 Antibody detects endogenous levels of total TIMP1.
RRID:
AB_2835315
引用格式: Affinity Biosciences Cat# AF7007, RRID:AB_2835315.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Clgi; Collagenase inhibitor; Collagenase inhibitor, Human; EPA; EPO; Erythroid Potentiating Activity; Erythroid-potentiating activity; Fibroblast collagenase inhibitor; FLJ90373; HCI; Human Collagenase Inhibitor; Metalloproteinase inhibitor 1; Metalloproteinase inhibitor 1 precursor; OTTHUMP00000023214; TIMP 1; TIMP; TIMP metallopeptidase inhibitor 1; TIMP-1; Timp1; TIMP1 protein; TIMP1_HUMAN; Tissue Inhibitor of Metalloproteinase 1; Tissue inhibitor of metalloproteinases 1; Tissue inhibitor of metalloproteinases; Ttissue inhibitor of metalloproteinase 1 erythroid potentiating activity collagenase inhibitor;

抗原和靶标

免疫原:

A synthesized peptide derived from human TIMP1, corresponding to a region within the internal amino acids.

基因/基因ID:
TIMP1(7076)
描述:
TIMP1 Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16.

研究领域

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.   (View pathway)

文献引用

1). Long-term simulated microgravity fosters carotid aging-like changes via Piezo1. Cardiovascular research, 2024 (PubMed: 38271270) [IF=10.2]
2). A biomaterial-based therapy using a sodium hyaluronate/bioglass composite hydrogel for the treatment of oral submucous fibrosis. Acta Biomaterialia, 2023 (PubMed: 36509401) [IF=9.4]
3). Autophagic-CTSB-inflammasome axis modulates hepatic stellate cells activation in arsenic-induced liver fibrosis. Chemosphere, 2020 (PubMed: 31669990) [IF=8.1]

Application: WB    Species: rat    Sample: HSC-t6 cells

Fig,5| NaAsO2-induced activation of NLRP3 inflammasome and HSCs were mediated by cytoplasmic CTSB. (A-C) HSC-t6 cells were pretreated with or without CA-074 me for 2 h and then treated with NaAsO2 for 24 h. (A) The cytoplasmic and lysosomal CTSB was detected by IF. White arrows indicate positive staining (Images are shown at 20×10 magnification). (B, C) The expression level of NLRP3,caspase-1 p20, IL-1β, Collagen-Ⅰ, a-SMA, MMP-1, and TIMP-1 were determined by Western blot.

Application: WB    Species: rat    Sample: livers

Fig.2 NaAsO2 induced autophagy, activation of NLRP3 inflammasome and 662 hepatic stellate cell in vivo. (A) The expression level of LC3 and p62 in rat livers 663 following exposure to NaAsO2. The protein fraction was analyzed by Western blot. (B) 664 The expression level of cytoplasmic CTSB in rat livers following exposure to NaAsO2. 665 The protein fraction was analyzed by Western blot. (C) The expression level of NLRP3 inflammasome-associated proteins in rat livers following exposure to NaAsO2. 667 The protein fraction was analyzed by Western blot. (D) The expression level of 668 Collagen- , α-SMA, MMP-1, and TIMP-1in rat livers following exposure to NaAsO2. 669 The protein fraction was analyzed by Western blot. (E) α-SMA was detected by IF. 670 White arrows indicate positive staining. Values are mean ± SD, and n = 5. *p < 0.05, 671 **p < 0.01 compared with the control group; Scale bar = 100 µm
4). Quercetin induces MGMT+ glioblastoma cells apoptosis via dual inhibition of Wnt3a/β-Catenin and Akt/NF-κB signaling pathways. Phytomedicine, 2023 (PubMed: 37451151) [IF=6.7]
5). Chrysin ameliorates synovitis and fibrosis of osteoarthritic fibroblast-like synoviocytes in rats through PERK/TXNIP/NLRP3 signaling. Frontiers in Pharmacology, 2023 (PubMed: 37021049) [IF=5.6]
6). Effects of the Leptin-Mediated MAPK/ERK Signaling Pathway on Collagen II Expression in Knee Cartilage of Newborn Male Mice from Obese Maternal Offspring. Biomolecules, 2022 (PubMed: 35327669) [IF=5.5]

Application: WB    Species: Mouse    Sample:

Figure 6. Inhibition of MAPK/ERK signaling pathway altered the expression of collagen II, MMP1, and TIMP1 in vitro. (A) Representative western blot bands of the expression of collagen II, MMP1, and TIMP1 in the cell cytoplasm and p-ERK in the nucleus induced by leptin, PD98059, or dimethyl sulfoxide (DMSO) (carrier for PD98059) in vitro (n = 3, chondrocytes obtained from three separate repeated experiments). (B) Statistical analysis of relative protein levels. Results are expressed as mean ± SD. ns not significant, * p < 0.05, ** p < 0.01, and *** p < 0.001.
7). Phillygenin Inhibits TGF-β1-induced Hepatic Stellate Cell Activation and Inflammation: Regulation of the Bax/Bcl-2 and Wnt/β-catenin Pathways. Inflammation, 2024 (PubMed: 38393550) [IF=4.5]

Application: WB    Species: Mouse    Sample: mHSC

Fig. 5 PHI inhibited TGF-β1-induced HSC activation in mHSCs and LX-2 cells. b Western blotting analyses of α-SMA, Collagen I, TIMP1, and MMP2 in mHSCs.
8). Exercise preconditioning mitigates brain injury after cerebral ischemia-reperfusion injury in rats by restraining TIMP1. Immunity, inflammation and disease, 2024 (PubMed: 39364701) [IF=3.2]
9). Protective Effect of the Total Alkaloid Extract from Bulbus Fritillariae pallidiflorae in a Mouse Model of Cigarette Smoke-Induced Chronic Obstructive Pulmonary Disease. International journal of chronic obstructive pulmonary disease, 2024 (PubMed: 38881716) [IF=2.7]
10). RNA sequencing reveals the potential mechanism of exercise preconditioning for cerebral ischemia reperfusion injury in rats. Brain and behavior, 2024 (PubMed: 38956886) [IF=2.6]

Application: WB    Species: Rat    Sample:

FIGURE 7 Exercise preconditioning (EP) suppressed TIMP1, SOCS3, ANGPTL4, CDO1, and SERPINE1 expressions of the cerebral cortices in middle cerebral artery occlusion (MCAO) rats. At 48 h after cerebral ischemia reperfusion injury (CIRI), TIMP1, SOCS3, ANGPTL4, CDO1, and SERPINE1 expression levels of cerebral cortices in each group were evaluated by qPCR (a) and Western blotting (b). # p < .05, ## p < .01 versus Sham. *p < .05, **p < .01 versus EP. Results were presented as mean ± SD. n = 3.
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