AFfirm™ NLRP3 Mouse monoclonal Antibody - #BF8029

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产品: NLRP3 小鼠 单克隆 抗体
货号: BF8029
描述: Mouse monoclonal antibody to NLRP3
应用: WB IHC
文献验证: WB, IHC
反应: Human, Mouse, Rat
蛋白ID: Q96P20

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MSDS
说明书
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Mouse IgG1
应用:
WB 1:500-1:3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Monoclonal [AFfirm8029(AFP21817)]
特异性:
NLRP3 Mouse monoclonal antibody detects endogenous levels of total NLRP3.
偶联:
Unconjugated.
纯化:
Affinity-chromatography.
保存:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

AGTAVPRL; AII/AVP; Angiotensin/vasopressin receptor AII/AVP like; Angiotensin/vasopressin receptor AII/AVP-like; C1orf7; Caterpiller protein 1.1; CIAS 1; CIAS1; CLR1.1; Cold autoinflammatory syndrome 1; Cold autoinflammatory syndrome 1 protein; Cryopyrin; Familial cold autoinflammatory syndrome; FCAS; FCU; LRR and PYD domains-containing protein 3; Muckle-Wells syndrome; MWS; NACHT; NACHT LRR and PYD containing protein 3; NALP 3; NALP3; NALP3_HUMAN; NLR family pyrin domain containing 3; NLRP3; PYPAF 1; PYPAF1; PYRIN containing APAF1 like protein 1; PYRIN-containing APAF1-like protein 1;

抗原和靶标

免疫原:

A synthesized peptide derived from Human NLRP3.

基因/基因ID:
NLRP3(114548)

研究领域

· Cellular Processes > Cell growth and death > Necroptosis.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Pertussis.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

文献引用

1). Blood Brain Barrier-Crossing Delivery of Felodipine Nanodrug Ameliorates Anxiety-Like Behavior and Cognitive Impairment in Alzheimer's Disease. Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2024 (PubMed: 38981028) [IF=15.1]
2). VSIG2 as a novel immunosuppressive ligand interacts with Nectin-2 to regulate T cell responses. Journal of neuroinflammation, 2025 (PubMed: 41350674) [IF=9.3]
3). Aerobic Exercise Restores Hippocampal Neurogenesis and Cognitive Function by Decreasing Microglia Inflammasome Formation Through Irisin/NLRP3 Pathway. Aging cell, 2025 (PubMed: 40192010) [IF=8.0]
4). An autonomous siRNA delivery system targeting NLRP3: implications in Parkinson's disease. BMC medicine, 2026 (PubMed: 41680766) [IF=7.0]
5). Protective Effects of Exogenous Melatonin Administration on White Fat Metabolism Disruption Induced by Aging and a High-Fat Diet in Mice. Antioxidants (Basel, Switzerland), 2024 (PubMed: 39765828) [IF=7.0]
6). Blocking CIRP protects against acute pancreatitis by improving mitochondrial function and suppressing pyroptosis in acinar cells. Cell death discovery, 2024 (PubMed: 38538578) [IF=7.0]
7). Artesunate inhibits macrophage-like phenotype switching of vascular smooth muscle cells and attenuates vascular inflammatory injury in atherosclerosis via NLRP3. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 2024 (PubMed: 38325261) [IF=6.9]

Application: WB    Species: Human    Sample: HVSMCs

Fig. 4. The anti-inflammatory effects of artesunate. (A) Immunofluorescence for NLRP3 and IL-1β expression in vitro. (B) Relative protein expression of NLRP3, Cleaved-CASP1, and Cleaved-IL-1β in vitro. *P 

Application: IF/ICC    Species: Mouse    Sample:

Fig. 2. Effects of artesunate on the expression of NLRP3, IL-1β, and NF-κB in the aorta. (A) Atherosclerosis and Artemisia annua L. related target genes and intersecting genes. (B) Pathway enrichment analysis of intersecting genes. (C) Relationship diagram of enrichment pathways and related genes. (D) Serum concentrations of TNF-α and IL-1β in the mice. (E) Immunohistochemistry for NF-κB and IL-1β expression in the aorta. (F) Immunofluorescence for NLRP3 expression in the aorta. **P 
8). DDIT3 drives nucleus pulposus cell PANoptosis and intervertebral disc degeneration progression. Apoptosis : an international journal on programmed cell death, 2026 (PubMed: 41518406) [IF=6.1]
9). Sodium-Glucose Cotransporter-2 Inhibitor Improves Renal Injury by Regulating the Redox Profile, Inflammatory Parameters, and Pyroptosis in an Experimental Model of Diabetic Kidney Disease. ACS pharmacology & translational science, 2025 (PubMed: 40370980) [IF=6.0]

Application: IHC    Species: Mouse    Sample:

Figure 5. Representative images of immunohistochemical analysis (cross sections) of NALP3, ASC, caspase-1, and gasdermin-D immunostaining (a) (400× magnification, scale bar = 50 μm), classification in intensity score of immunodetection of NALP3, ASC and caspase-1 (b), and immunoexpression of gasdermin D (%) (c), in the kidney of animal models of DKD. For intensity score, + indicates weak staining, ++ indicates moderate staining. Data are presented as mean ± standard deviation. Statistical analysis was performed using two-way ANOVA followed by the Bonferroni posthoc test.
10). Vitexin mitigates oxidative stress, mitochondrial damage, pyroptosis and regulates small nucleolar RNA host gene 1/DNA methyltransferase 1/microRNA-495 axis in sepsis-associated acute lung injury. Inflammopharmacology, 2024 (PubMed: 39641834) [IF=5.8]
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