AFfirm™ NLRP3 Mouse monoclonal Antibody - #BF8029

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产品: NLRP3 小鼠 单克隆 抗体
货号: BF8029
描述: Mouse monoclonal antibody to NLRP3
应用: WB IHC
文献验证: WB
反应: Human, Mouse, Rat
蛋白号: Q96P20

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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说明书
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Mouse IgG1
应用:
WB 1:500-1:3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Monoclonal [AFfirm8029(AFP21817)]
特异性:
NLRP3 Mouse monoclonal antibody detects endogenous levels of total NLRP3.
偶联:
Unconjugated.
纯化:
Affinity-chromatography.
保存:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

AGTAVPRL; AII/AVP; Angiotensin/vasopressin receptor AII/AVP like; Angiotensin/vasopressin receptor AII/AVP-like; C1orf7; Caterpiller protein 1.1; CIAS 1; CIAS1; CLR1.1; Cold autoinflammatory syndrome 1; Cold autoinflammatory syndrome 1 protein; Cryopyrin; Familial cold autoinflammatory syndrome; FCAS; FCU; LRR and PYD domains-containing protein 3; Muckle-Wells syndrome; MWS; NACHT; NACHT LRR and PYD containing protein 3; NALP 3; NALP3; NALP3_HUMAN; NLR family pyrin domain containing 3; NLRP3; PYPAF 1; PYPAF1; PYRIN containing APAF1 like protein 1; PYRIN-containing APAF1-like protein 1;

抗原和靶标

免疫原:

A synthesized peptide derived from Human NLRP3.

基因/基因ID:
NLRP3(114548)

研究领域

· Cellular Processes > Cell growth and death > Necroptosis.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Pertussis.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

文献引用

1). Blood Brain Barrier-Crossing Delivery of Felodipine Nanodrug Ameliorates Anxiety-Like Behavior and Cognitive Impairment in Alzheimer's Disease. Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2024 (PubMed: 38981028) [IF=15.1]
2). Aerobic Exercise Restores Hippocampal Neurogenesis and Cognitive Function by Decreasing Microglia Inflammasome Formation Through Irisin/NLRP3 Pathway. Aging cell, 2025 (PubMed: 40192010) [IF=8.0]
3). Protective Effects of Exogenous Melatonin Administration on White Fat Metabolism Disruption Induced by Aging and a High-Fat Diet in Mice. Antioxidants (Basel, Switzerland), 2024 (PubMed: 39765828) [IF=7.0]
4). Blocking CIRP protects against acute pancreatitis by improving mitochondrial function and suppressing pyroptosis in acinar cells. Cell death discovery, 2024 (PubMed: 38538578) [IF=7.0]
5). Artesunate inhibits macrophage-like phenotype switching of vascular smooth muscle cells and attenuates vascular inflammatory injury in atherosclerosis via NLRP3. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 2024 (PubMed: 38325261) [IF=6.9]

Application: WB    Species: Human    Sample: HVSMCs

Fig. 4. The anti-inflammatory effects of artesunate. (A) Immunofluorescence for NLRP3 and IL-1β expression in vitro. (B) Relative protein expression of NLRP3, Cleaved-CASP1, and Cleaved-IL-1β in vitro. *P 

Application: IF/ICC    Species: Mouse    Sample:

Fig. 2. Effects of artesunate on the expression of NLRP3, IL-1β, and NF-κB in the aorta. (A) Atherosclerosis and Artemisia annua L. related target genes and intersecting genes. (B) Pathway enrichment analysis of intersecting genes. (C) Relationship diagram of enrichment pathways and related genes. (D) Serum concentrations of TNF-α and IL-1β in the mice. (E) Immunohistochemistry for NF-κB and IL-1β expression in the aorta. (F) Immunofluorescence for NLRP3 expression in the aorta. **P 
6). Vitexin mitigates oxidative stress, mitochondrial damage, pyroptosis and regulates small nucleolar RNA host gene 1/DNA methyltransferase 1/microRNA-495 axis in sepsis-associated acute lung injury. Inflammopharmacology, 2024 (PubMed: 39641834) [IF=5.8]
7). Inhibition of HDAC6 alleviates cancer‑induced bone pain by reducing the activation of NLRP3 inflammasome. International journal of molecular medicine, 2024 (PubMed: 37997785) [IF=5.7]

Application: WB    Species: Rat    Sample: spinal cord

Figure 5 Effect of TSA on NLRP3 inflammasome activation and NF-κB. (A) Representative immunofluorescence staining images and (B) quantitative analysis for NLRP3, caspase-1 and NF-κB in the spinal cord. Scale bar, 20 μm. (C) Representative western blot bands and (E) quantitative analysis of NLRP3, caspase-1 and NF-κB proteins in spinal cord. (D and F) HDAC6 interaction with NLRP3 detected by immunoprecipitation assay. Spinal tissue lysates were immunoprecipitated with anti-HDAC6 and anti-NLRP3 antibodies and immunoblots were probed with anti-NLRP3 and anti-HDAC6 antibodies. The input panel showed target proteins prior to immunoprecipitation in the extracts. Data were presented as mean ± SD (n=3). *P

Application: IF/ICC    Species: Rat    Sample: spinal cord

Figure 5 Effect of TSA on NLRP3 inflammasome activation and NF-κB. (A) Representative immunofluorescence staining images and (B) quantitative analysis for NLRP3, caspase-1 and NF-κB in the spinal cord. Scale bar, 20 μm. (C) Representative western blot bands and (E) quantitative analysis of NLRP3, caspase-1 and NF-κB proteins in spinal cord. (D and F) HDAC6 interaction with NLRP3 detected by immunoprecipitation assay. Spinal tissue lysates were immunoprecipitated with anti-HDAC6 and anti-NLRP3 antibodies and immunoblots were probed with anti-NLRP3 and anti-HDAC6 antibodies. The input panel showed target proteins prior to immunoprecipitation in the extracts. Data were presented as mean ± SD (n=3). *P
8). HCMV-IE2 promotes atherosclerosis by inhibiting vascular smooth muscle cells' pyroptosis. Frontiers in microbiology, 2023 (PubMed: 37234524) [IF=5.2]
9). Patchouli alcohol alleviates metabolic dysfunction-associated steatohepatitis via inhibiting mitochondria-associated endoplasmic reticulum membrane disruption-induced hepatic steatosis and inflammation in rats. International immunopharmacology, 2024 (PubMed: 38971107) [IF=4.8]
10). Ginsenoside Rg1 attenuates T2DM-induced renal damage and fibrosis by inhibiting TRPC6-ChREBP-TXNIP signaling. Journal of ethnopharmacology, 2025 (PubMed: 40311716) [IF=4.8]
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