产品: XPF 抗体
货号: AF0295
描述: Rabbit polyclonal antibody to XPF
应用: WB IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Sheep, Rabbit, Dog, Chicken
分子量: 103kDa; 104kD(Calculated).
蛋白号: Q92889
RRID: AB_2833461

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(92%), Sheep(92%), Rabbit(92%), Dog(100%), Chicken(92%)
克隆:
Polyclonal
特异性:
XPF Antibody detects endogenous levels of total XPF.
RRID:
AB_2833461
引用格式: Affinity Biosciences Cat# AF0295, RRID:AB_2833461.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

DNA excision repair protein ERCC 4; DNA excision repair protein ERCC-4; DNA excision repair protein ERCC4; DNA repair endonuclease XPF; DNA repair protein complementing XP F cells; DNA repair protein complementing XP-F cells; ERCC 11; ERCC 4; ERCC11; ERCC4; Excision repair complementing defective in Chinese hamster; Excision repair cross complementing rodent repair deficiency complementation group 4; excision repair cross-complementation group 4; FANCQ; RAD 1; RAD1; Xeroderma pigmentosum complementation group F; Xeroderma pigmentosum group F complementing protein; Xeroderma pigmentosum group F-complementing protein; Xeroderma pigmentosum VI; XP, group G; XP6; XPF_HUMAN;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
描述:
ERCC4 Structure-specific DNA repair endonuclease responsible for the 5-prime incision during DNA repair. Involved in homologous recombination that assists in removing interstrand cross-link. Belongs to the XPF family. Heterodimer composed of ERCC1 and XPF/ERCC4.
序列:
MESGQPARRIAMAPLLEYERQLVLELLDTDGLVVCARGLGADRLLYHFLQLHCHPACLVLVLNTQPAEEEYFINQLKIEGVEHLPRRVTNEITSNSRYEVYTQGGVIFATSRILVVDFLTDRIPSDLITGILVYRAHRIIESCQEAFILRLFRQKNKRGFIKAFTDNAVAFDTGFCHVERVMRNLFVRKLYLWPRFHVAVNSFLEQHKPEVVEIHVSMTPTMLAIQTAILDILNACLKELKCHNPSLEVEDLSLENAIGKPFDKTIRHYLDPLWHQLGAKTKSLVQDLKILRTLLQYLSQYDCVTFLNLLESLRATEKAFGQNSGWLFLDSSTSMFINARARVYHLPDAKMSKKEKISEKMEIKEGEETKKELVLESNPKWEALTEVLKEIEAENKESEALGGPGQVLICASDDRTCSQLRDYITLGAEAFLLRLYRKTFEKDSKAEEVWMKFRKEDSSKRIRKSHKRPKDPQNKERASTKERTLKKKKRKLTLTQMVGKPEELEEEGDVEEGYRREISSSPESCPEEIKHEEFDVNLSSDAAFGILKEPLTIIHPLLGCSDPYALTRVLHEVEPRYVVLYDAELTFVRQLEIYRASRPGKPLRVYFLIYGGSTEEQRYLTALRKEKEAFEKLIREKASMVVPEEREGRDETNLDLVRGTASADVSTDTRKAGGQEQNGTQQSIVVDMREFRSELPSLIHRRGIDIEPVTLEVGDYILTPEMCVERKSISDLIGSLNNGRLYSQCISMSRYYKRPVLLIEFDPSKPFSLTSRGALFQEISSNDISSKLTLLTLHFPRLRILWCPSPHATAELFEELKQSKPQPDAATALAITADSETLPESEKYNPGPQDFLLKMPGVNAKNCRSLMHHVKNIAELAALSQDELTSILGNAANAKQLYDFIHTSFAEVVSKGKGKK

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Dog
100
Bovine
92
Sheep
92
Chicken
92
Rabbit
92
Xenopus
77
Horse
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q92889 作为底物

Site PTM Type Enzyme
Y191 Phosphorylation
K260 Acetylation
K260 Ubiquitination
K264 Ubiquitination
K282 Ubiquitination
K289 Acetylation
K371 Ubiquitination
K380 Ubiquitination
K389 Ubiquitination
K445 Ubiquitination
K452 Ubiquitination
S465 Phosphorylation
T493 Phosphorylation
K500 Acetylation
S520 Phosphorylation
S521 Phosphorylation
S524 Phosphorylation
K632 Ubiquitination
T660 Phosphorylation
S662 Phosphorylation
T669 Phosphorylation
T710 Phosphorylation
Y742 Phosphorylation
Y752 Phosphorylation
K753 Ubiquitination
K843 Ubiquitination
K861 Ubiquitination

研究背景

功能:

Catalytic component of a structure-specific DNA repair endonuclease responsible for the 5-prime incision during DNA repair. Involved in homologous recombination that assists in removing interstrand cross-link.

细胞定位:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Heterodimer composed of ERCC1 and XPF/ERCC4. Interacts with SLX4/BTBD12; this interaction is direct and links the ERCC1-XPF/ERCC1 complex to SLX4, which may coordinate the action of the structure-specific endonuclease during DNA repair.

蛋白家族:

Belongs to the XPF family.

研究领域

· Genetic Information Processing > Replication and repair > Nucleotide excision repair.

· Genetic Information Processing > Replication and repair > Fanconi anemia pathway.

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