STAT4 Antibody - #AF6441

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产品: STAT4 抗体
货号: AF6441
描述: Rabbit polyclonal antibody to STAT4
应用: WB IHC IF/ICC IP
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Horse, Rabbit, Chicken
蛋白号: Q14765
RRID: AB_2835265

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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WB Handbook
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IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IP, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
STAT4 Antibody detects endogenous levels of total STAT4.
RRID:
AB_2835265
引用格式: Affinity Biosciences Cat# AF6441, RRID:AB_2835265.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Signal transducer and activator of transcription 4; SLEB11; STAT4; STAT4_HUMAN;

抗原和靶标

免疫原:

A synthesized peptide derived from human STAT4, corresponding to a region within C-terminal amino acids.

基因/基因ID:
STAT4(6775)
描述:
The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators.

研究领域

· Cellular Processes > Cell growth and death > Necroptosis.   (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Immune diseases > Inflammatory bowel disease (IBD).

· Organismal Systems > Immune system > Th1 and Th2 cell differentiation.   (View pathway)

文献引用

1). Oncostatin M Induces IFITM1 Expression to Inhibit Hepatitis B Virus Replication Via JAK-STAT Signaling. Cellular and molecular gastroenterology and hepatology, 2024 (PubMed: 37879404) [IF=7.1]

Application: WB    Species: human    Sample: HepG2.2.15 cells

Figure 5. OSM suppresses HBV replication through the JAK-STAT signaling pathway. (A) Extracts from HepG2.2.15 cells treated with OSM (20 ng/mL) for 0∼60 minutes were analyzed for STAT activation. (B) HepG2.2.15 cells were pretreated with ruxolitinib (1 μM) and the AG490 (10 μM) for 1 hour and then were treated with OSM (20 ng/mL) for 10 minutes. The phosphorylation levels of STAT1, STAT3, and STAT5 were examined by immunoblotting. (C, E) The supernatant HBsAg and HBV DNA levels were examined in the indicated groups. One-way analysis of variance. (D) The inhibition effects of OSM on intracellular HBV RNAs and HBV antigens were analyzed in HepG2.2.15 cells pretreated with or without ruxolitinib. Inhibition ratios of OSM for intracellular pgRNA and total RNA for the indicated cell lines were calculated as (1 - OSM-treatment/sham-treatment) ×100. Relative gray values of HBsAg and HBcAg were analyzed by Image J and normalized to β-actin. Unpaired t test. (F) The expression levels of IRFs in HepG2.2.15 cells treated with OSM were examined by quantitative polymerase chain reaction and immunoblotting. Unpaired t test. (G) STAT1-IRF9 heterodimer formation was examined by coimmunoprecipitation in Huh7-1.3 cells treated with OSM. ∗P < .05, ∗∗P < .01, and ∗∗∗P < .001.

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