HDAC1 Antibody - #AF6433

Fig. 3 Histological and western blotting analysis of microglial activation, pro-inflammatory cytokines, and histone deacetylase (Hdac)1/2 levels. A Immunofluorescence staining of Iba1 in the cortex and hippocampus. B Comparisons of the numbers of Iba1-positive microglia in the cortex and hippocampus (×40 objective). C Chemiluminescence images of IL-6, Hdac1, and GAPDH. D Comparisons of the IL-6/GAPDH, Hdac1/ GAPDH ratios. E Chemiluminescence images of TNF-α, Hdac2, and GAPDH. F Comparisons of the TNF-α/GAPDH, Hdac2/GAPDH ratios. Each dataset is expressed as mean ± SD. *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001; ****P ≤ 0.0001. n = 6 mice.

Figure 10 Protein expression levels of 10 prognostic genes in human HCC and normal liver tissues measured by IHC (*P 

Figure 4 A combination of Chidamide and Bortezomib increases production of ROS dependent DNA damage and the changes of cell apoptosis and cycle pathway in MM cells. (A) ARP-1 cells were pretreated with or without NAC (15.0 mmol/L) for 2 hours at 37°C and then incubated with CHI (1.0 µmol/L) and/or BTZ (5.0 nmol/L) for 24 hours, then ROS generation was detected. (B) ARP-1 cells were pretreated with or without 15 mmol/L NAC and then treated with Chidamide or Bortezomib alone or in combination, and cell viabilities were evaluated using CCK-8 assays. (C) The expression of γ-H2AX in ARP-1 cells treated with CHI (1.0 µmol/L) and/or BTZ (5.0 nmol/L) were determined by Western blot. (D) Representative images of γ-H2AX (Red) and nuclei (Blue) in ARP-1 cells treated with single agent or combination for 24 hours by immunofluorescence assay. Scale bars represent 20 µm. (E, F) Western blot analysis of the expressions of cleaved caspase3, cleaved caspase8, cleaved PARP-1 and HDAC1 in XG1 (E) and ARP-1 (F) cells after 48 hours treatment with single agent or in combination. Error bars indicate mean ± SD. **p < 0.01.

Fig. 1 Expression of HDAC1 in AS fibroblasts. A The protein expression of HDAC1 in AS fibroblasts was detected by western blot analysis. B Densitometry analysis of protein expression. C Gene expression of HDAC1 in AS fibroblasts was detected by RT-PCR analysis. Data were shown as mean ± SD. *P < 0.05, **P < 0.01 and ***P < 0.001, compared with the control group