NLRP3 Antibody - #DF15549

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产品: NLRP3 抗体
货号: DF15549
描述: Rabbit polyclonal antibody to NLRP3
应用: WB IHC
文献验证: WB, IHC
反应: Human, Mouse, Rat
蛋白号: Q96P20

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   规格 价格 库存
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
IHC 1:50-1:200, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
NLRP3 Antibody detects endogenous levels of NLRP3.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

AGTAVPRL; AII/AVP; Angiotensin/vasopressin receptor AII/AVP like; Angiotensin/vasopressin receptor AII/AVP-like; C1orf7; Caterpiller protein 1.1; CIAS 1; CIAS1; CLR1.1; Cold autoinflammatory syndrome 1; Cold autoinflammatory syndrome 1 protein; Cryopyrin; Familial cold autoinflammatory syndrome; FCAS; FCU; LRR and PYD domains-containing protein 3; Muckle-Wells syndrome; MWS; NACHT; NACHT LRR and PYD containing protein 3; NALP 3; NALP3; NALP3_HUMAN; NLR family pyrin domain containing 3; NLRP3; PYPAF 1; PYPAF1; PYRIN containing APAF1 like protein 1; PYRIN-containing APAF1-like protein 1;

抗原和靶标

免疫原:

A synthesized peptide derived from human NLRP3.

基因/基因ID:
NLRP3(114548)

研究领域

· Cellular Processes > Cell growth and death > Necroptosis.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Pertussis.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

文献引用

1). Interorgan communication in neurogenic heterotopic ossification: the role of brain-derived extracellular vesicles. Bone research, 2024 (PubMed: 38383487) [IF=14.3]
2). ChemR23 activation attenuates cognitive impairment in chronic cerebral hypoperfusion by inhibiting NLRP3 inflammasome-induced neuronal pyroptosis. Cell death & disease, 2023 (PubMed: 37932279) [IF=8.1]

Application: IF/ICC    Species: Rat    Sample:

Fig. 4: Effects of ChemR23 activation with RvE1 or C-9 on neuronal pyroptosis in CCH rats. A Representative transmission electron micrographs of hippocampal neurons in different treatment groups. Black arrowhead: membrane pores. Scale bar: 2 μm. n = 3 per group. B, C Representative double immunofluorescence staining of GSDMD/NeuN and NLRP3/NeuN in the rat hippocampus. Scale bar: 20 μm. n = 3 per group. D, E Quantitative statistics of GSDMD/NeuN or NLRP3/NeuN double-positive cells in rat hippocampus. n = 3 per group. F, G ELISA results of IL-1 and IL-18 analysis in the rat hippocampus. n = 4 per group. H, I Representative immunoblotting bands and the expression levels of NLRP3, GSDMD-N, Caspase-1 p20 and ASC in the rat hippocampus. n = 4 per group. Data are presented as the mean ± SEM. **P 

Application: WB    Species: Rat    Sample:

Fig. 4: Effects of ChemR23 activation with RvE1 or C-9 on neuronal pyroptosis in CCH rats. A Representative transmission electron micrographs of hippocampal neurons in different treatment groups. Black arrowhead: membrane pores. Scale bar: 2 μm. n = 3 per group. B, C Representative double immunofluorescence staining of GSDMD/NeuN and NLRP3/NeuN in the rat hippocampus. Scale bar: 20 μm. n = 3 per group. D, E Quantitative statistics of GSDMD/NeuN or NLRP3/NeuN double-positive cells in rat hippocampus. n = 3 per group. F, G ELISA results of IL-1 and IL-18 analysis in the rat hippocampus. n = 4 per group. H, I Representative immunoblotting bands and the expression levels of NLRP3, GSDMD-N, Caspase-1 p20 and ASC in the rat hippocampus. n = 4 per group. Data are presented as the mean ± SEM. **P 
3). Loureirin B Reduces Insulin Resistance and Chronic Inflammation in a Rat Model of Polycystic Ovary Syndrome by Upregulating GPR120 and Activating the LKB1/AMPK Signaling Pathway. International journal of molecular sciences, 2024 (PubMed: 39456928) [IF=5.6]
4). Gut microbial metabolite indole-3-propionic acid alleviates polycystic ovary syndrome in mice by regulating the AhR-NLRP3 axis. International immunopharmacology, 2025 (PubMed: 39826449) [IF=4.8]
5). Dapagliflozin attenuates skeletal muscle atrophy in diabetic nephropathy mice through suppressing Gasdermin D-mediated pyroptosis. International immunopharmacology, 2025 (PubMed: 39837016) [IF=4.8]
6). MIF aggravates experimental autoimmune prostatitis through activation of the NLRP3 inflammasome via the PI3K/AKT pathway. International immunopharmacology, 2024 (PubMed: 39153310) [IF=4.8]
7). PRG4 mitigates hemorrhagic shock-induced cardiac injury by inhibiting mitochondrial dysregulation, oxidative stress and NLRP3-mediated pyroptosis. International immunopharmacology, 2024 (PubMed: 38897120) [IF=4.8]
8). MCC950 alleviates seizure severity and angiogenesis by inhibiting NLRP3/ IL-1β signaling pathway-mediated pyroptosis in mouse model of epilepsy. International immunopharmacology, 2024 (PubMed: 38039716) [IF=4.8]
9). Gandouling Regulates Ferroptosis and Improves Neuroinflammation in Wilson's Disease Through the LCN2/NLRP3 Signaling Pathway. Journal of inflammation research, 2024 (PubMed: 39193124) [IF=4.5]
10). Asprosin promotes vascular inflammation via TLR4-NFκB-mediated NLRP3 inflammasome activation in hypertension. Heliyon, 2024 (PubMed: 38841464) [IF=4.0]

Application: WB    Species: Mouse    Sample: VSMCs

Fig. 2 Effects of asprosin overexpression (OE) or knockdown (KD) on related protein expressions in VSMCs. A, asprosin protein. B, NLRP3, ASC, caspase-1, IL-1β protein expressions. Cells were incubated for 48 h with asprosin OE plasmid (2 μg) or asprosin siRNA (50 nM). Two-way ANOVA followed by Bonferroni test. n = 4. *P < 0.05; #P < 0.05 vs WKY.
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