NLRP3 Antibody - #DF15549

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产品: NLRP3 抗体
货号: DF15549
描述: Rabbit polyclonal antibody to NLRP3
应用: WB IHC
文献验证: WB, IHC
反应: Human, Mouse, Rat
蛋白号: Q96P20

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   规格 价格 库存
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
IHC 1:50-1:200, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
NLRP3 Antibody detects endogenous levels of NLRP3.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

AGTAVPRL; AII/AVP; Angiotensin/vasopressin receptor AII/AVP like; Angiotensin/vasopressin receptor AII/AVP-like; C1orf7; Caterpiller protein 1.1; CIAS 1; CIAS1; CLR1.1; Cold autoinflammatory syndrome 1; Cold autoinflammatory syndrome 1 protein; Cryopyrin; Familial cold autoinflammatory syndrome; FCAS; FCU; LRR and PYD domains-containing protein 3; Muckle-Wells syndrome; MWS; NACHT; NACHT LRR and PYD containing protein 3; NALP 3; NALP3; NALP3_HUMAN; NLR family pyrin domain containing 3; NLRP3; PYPAF 1; PYPAF1; PYRIN containing APAF1 like protein 1; PYRIN-containing APAF1-like protein 1;

抗原和靶标

免疫原:

A synthesized peptide derived from human NLRP3.

基因/基因ID:
NLRP3(114548)

研究领域

· Cellular Processes > Cell growth and death > Necroptosis.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Pertussis.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

文献引用

1). Interorgan communication in neurogenic heterotopic ossification: the role of brain-derived extracellular vesicles. Bone research, 2024 (PubMed: 38383487) [IF=14.3]
2). ChemR23 activation attenuates cognitive impairment in chronic cerebral hypoperfusion by inhibiting NLRP3 inflammasome-induced neuronal pyroptosis. Cell death & disease, 2023 (PubMed: 37932279) [IF=8.1]

Application: IF/ICC    Species: Rat    Sample:

Fig. 4: Effects of ChemR23 activation with RvE1 or C-9 on neuronal pyroptosis in CCH rats. A Representative transmission electron micrographs of hippocampal neurons in different treatment groups. Black arrowhead: membrane pores. Scale bar: 2 μm. n = 3 per group. B, C Representative double immunofluorescence staining of GSDMD/NeuN and NLRP3/NeuN in the rat hippocampus. Scale bar: 20 μm. n = 3 per group. D, E Quantitative statistics of GSDMD/NeuN or NLRP3/NeuN double-positive cells in rat hippocampus. n = 3 per group. F, G ELISA results of IL-1 and IL-18 analysis in the rat hippocampus. n = 4 per group. H, I Representative immunoblotting bands and the expression levels of NLRP3, GSDMD-N, Caspase-1 p20 and ASC in the rat hippocampus. n = 4 per group. Data are presented as the mean ± SEM. **P 

Application: WB    Species: Rat    Sample:

Fig. 4: Effects of ChemR23 activation with RvE1 or C-9 on neuronal pyroptosis in CCH rats. A Representative transmission electron micrographs of hippocampal neurons in different treatment groups. Black arrowhead: membrane pores. Scale bar: 2 μm. n = 3 per group. B, C Representative double immunofluorescence staining of GSDMD/NeuN and NLRP3/NeuN in the rat hippocampus. Scale bar: 20 μm. n = 3 per group. D, E Quantitative statistics of GSDMD/NeuN or NLRP3/NeuN double-positive cells in rat hippocampus. n = 3 per group. F, G ELISA results of IL-1 and IL-18 analysis in the rat hippocampus. n = 4 per group. H, I Representative immunoblotting bands and the expression levels of NLRP3, GSDMD-N, Caspase-1 p20 and ASC in the rat hippocampus. n = 4 per group. Data are presented as the mean ± SEM. **P 
3). Astragalin Inhibits Oxidative Stress-Induced Pyroptosis and Apoptosis in Mouse Models of Renal Ischemia/Reperfusion Injury by Activating the SIRT1/Nrf2 Pathway. Phytotherapy research : PTR, 2025 (PubMed: 40902986) [IF=6.1]
4). Loureirin B Reduces Insulin Resistance and Chronic Inflammation in a Rat Model of Polycystic Ovary Syndrome by Upregulating GPR120 and Activating the LKB1/AMPK Signaling Pathway. International journal of molecular sciences, 2024 (PubMed: 39456928) [IF=5.6]

Application: WB    Species: Rat    Sample:

Figure 11. Effects of LrB on NLRP3 and Caspase-1 levels in the ovaries of PCOS-IR rats. The values are means ± SDs; n = 3. (A) Western blot analysis of NLRP3 and GAPDH in ovarian tissues at four weeks of treatment. (B) Quantitative analysis of NLRP3 levels in ovarian tissues at four weeks of treatment. (C) Expression levels of NLRP3 mRNA in ovarian tissues at four weeks of treatment. (D) Western blot analysis of Caspase-1 and GAPDH in ovarian tissues at four weeks of treatment. (E) Quantitative analysis of Caspase-1 levels in ovarian tissues at four weeks of treatment. (F) Expression levels of Caspase-1 mRNA in ovarian tissues at four weeks of treatment. Relative to the normal group, ## p < 0.01; relative to the PCOS-IR group
5). PRG4 mitigates hemorrhagic shock-induced cardiac injury by inhibiting mitochondrial dysregulation, oxidative stress and NLRP3-mediated pyroptosis. International immunopharmacology, 2024 (PubMed: 38897120) [IF=4.8]
6). MIF aggravates experimental autoimmune prostatitis through activation of the NLRP3 inflammasome via the PI3K/AKT pathway. International immunopharmacology, 2024 (PubMed: 39153310) [IF=4.8]
7). MCC950 alleviates seizure severity and angiogenesis by inhibiting NLRP3/ IL-1β signaling pathway-mediated pyroptosis in mouse model of epilepsy. International immunopharmacology, 2024 (PubMed: 38039716) [IF=4.8]
8). Dapagliflozin attenuates skeletal muscle atrophy in diabetic nephropathy mice through suppressing Gasdermin D-mediated pyroptosis. International immunopharmacology, 2025 (PubMed: 39837016) [IF=4.8]
9). Gut microbial metabolite indole-3-propionic acid alleviates polycystic ovary syndrome in mice by regulating the AhR-NLRP3 axis. International immunopharmacology, 2025 (PubMed: 39826449) [IF=4.8]
10). Gandouling Regulates Ferroptosis and Improves Neuroinflammation in Wilson's Disease Through the LCN2/NLRP3 Signaling Pathway. Journal of inflammation research, 2024 (PubMed: 39193124) [IF=4.5]
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