BCL-XL Antibody - #AF6414

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产品: BCL-XL 抗体
货号: AF6414
描述: Rabbit polyclonal antibody to BCL-XL
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog
蛋白号: Q07817
RRID: AB_2835244

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
BCL-XL Antibody detects endogenous levels of total BCL-XL.
RRID:
AB_2835244
引用格式: Affinity Biosciences Cat# AF6414, RRID:AB_2835244.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Apoptosis regulator Bcl X; Apoptosis regulator Bcl-X; Apoptosis regulator BclX; B cell lymphoma 2 like; B2CL1_HUMAN; Bcl 2 like 1 protein; Bcl X; Bcl xL; BCL XL/S; Bcl xS; Bcl-2-like protein 1; Bcl2 Like 1; Bcl2 related gene; Bcl2-L-1; BCL2L; Bcl2l1; BCLX; BclXL; BclXs; DKFZp781P2092; PPP1R52; Protein phosphatase 1 regulatory subunit 52;

抗原和靶标

免疫原:

A synthesized peptide derived from human BCL-XL, corresponding to a region within N-terminal amino acids.

基因/基因ID:
BCL2L1(598)
描述:
The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities.

研究领域

· Cellular Processes > Transport and catabolism > Autophagy - animal.   (View pathway)

· Cellular Processes > Cell growth and death > Apoptosis.   (View pathway)

· Cellular Processes > Cell growth and death > Apoptosis - multiple species.   (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway.   (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.

· Human Diseases > Drug resistance: Antineoplastic > Platinum drug resistance.

· Human Diseases > Neurodegenerative diseases > Amyotrophic lateral sclerosis (ALS).

· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

· Human Diseases > Cancers: Specific types > Pancreatic cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Chronic myeloid leukemia.   (View pathway)

· Human Diseases > Cancers: Specific types > Small cell lung cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma.   (View pathway)

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

文献引用

1). Astragaloside IV attenuates myocardial dysfunction in diabetic cardiomyopathy rats through downregulation of CD36-mediated ferroptosis. Phytotherapy Research, 2023 (PubMed: 36882189) [IF=6.1]
2). Poly(ADP-ribose) polymerase family member 14 promotes functional recovery after spinal cord injury through regulating microglia M1/M2 polarization via STAT1/6 pathway. Neural Regeneration Research, 2023 (PubMed: 36751810) [IF=5.9]

Application: WB    Species: Mouse    Sample:

Figure 3 PARP14 deficiency exacerbates SCI-induced neuronal apoptosis at 7 days post-SCI. (A) Representative images and quantitative analysis of Nissl staining in each group. Lv-shPARP14 injection increased SCI-induced neuronal loss. (B, C) Representative images and quantitative analysis showing TUNEL+/NeuN+ immunofluorescence staining. Lv-shPARP14 injection increased SCI-induced neuronal apoptosis. White arrows indicate TUNEL+ (green, apoptosis cells)/NeuN+ (red, Cy3-labeled, neuronal marker) cells. Scale bars: 50 µm in A and B. (D) Western blot analysis of cleaved caspase 3, bax, Bcl-2, and Bcl-xl in each group. Lv-shPARP14 injection enhanced the SCI-induced increase in cleaved caspase 3 and bax (pro-apoptotic factors) expression and decrease in Bcl-2 and Bcl-xl (anti-apoptotic factors) expression. Values are shown as mean ± SD (n = 6). *P < 0.05, **P < 0.01 (one-way analysis of variance followed by Tukey’s post hoc test). Images were taken from the gray matter ventral horn at the injury site. Spinal cord tissues from the injury site were used for western blot detection. DAPI: 4′,6-Diamidino-2-phenylindole; PARP14: poly(ADP-ribose)polymerase, member 14; SCI: spinal cord injury; TUNEL: terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling.
3). Effective extraction of Xuetongsu and its role in preventing RA synovial hyperplasia by targeting synovial cell migration and apoptosis. Scientific reports, 2024 (PubMed: 39375373) [IF=3.8]
4). A tumor-suppressing role of TSPYL2 in thyroid cancer: Through interacting with SIRT1 and repressing SIRT1/AKT pathway. Experimental cell research, 2023 (PubMed: 37696385) [IF=3.3]
5). Inotodiol induces hepatocellular carcinoma apoptosis by activation of MAPK/ERK pathway. PloS one, 2025 (PubMed: 39879230) [IF=2.9]
6). miR-374 improves cerebral ischemia reperfusion injury by targeting Wnt5a. EXPERIMENTAL ANIMALS, 2021 (PubMed: 33116025) [IF=2.2]

Application: WB    Species: rat    Sample: brain

Fig. 3. |Overexpression of miR-374 attenuated cerebral IR-induced apoptosis. (B) Western blot showing expression of apoptosis-related proteins (BCL-XL, BCL-2, and BAX) in brain tissues. Data are presented as the mean ± SD, and they were analyzed by unpaired t-test. Comparison of the sham group with the IR group and the IR + NC agomir group with the IR + miR-374 agomir group: *P<0.05; ***P<0.001; ****P<0.0001.
7). Irisin Enhances Doxorubicin-Induced Cell Apoptosis in Pancreatic Cancer by Inhibiting the PI3K/AKT/NF-κB Pathway. MEDICAL SCIENCE MONITOR, 2019 (PubMed: 31412018) [IF=2.2]

Application: WB    Species: human    Sample: MIA PaCa-2 and BxPC-3 cells

Figure 3.| Irisin enhances DOX-induced apoptosis in PC cells. MIA PaCa-2 cells were exposed to 100 nM irisin alone, 0.75 μg/mL DOX alone, or DOX combined with irisin for 24 h. BxPC-3 cells were exposed to 100 nM irisin alone, 1.5 μg/mL DOX alone, or DOX combined with irisin for 24 h. (C) Western blot analysis of the levels of cleaved PARP, cleaved caspase-3, BCL-2, and BCL-xL protein in MIA PaCa-2 (a) and BxPC-3 (b) cells. b-actin served as the loading control.
8). Phenylethanoid glycoside verbascoside ameliorates podocyte injury of diabetic kidney disease by regulating NR4A1-LKB1-AMPK signaling. Acta Materia Medica, 2023

Application: WB    Species: Mouse    Sample:

Figure 5 | NR4A1 knockdown alleviated cell stress responses in high glucose-triggered podocytes. MPC5 cells were transfected with negative control (NC) siRNA or NR4A1 siRNA for 24 h with or without HG or verbascoside treatment. Then, the protein (a) and mRNA (d–f) levels of IL-6, CXCL10, and MCP-1 were analyzed. (b) The levels of Bcl-2, cleaved caspase-3/caspase-3, Bax, and Bcl-xL protein were detected by Western blot. (g–i) The levels of Bcl-2, Bax, and Bcl-xL mRNA were analyzed by RT-PCR. (c) Western blot showed the levels of Beclin-1, LC3-II, Atg5, and p62 protein expression. All data are represented as the mean ± SD (n =3). ****P < 0.0001 compared with the HG group. ## P < 0.01, ### P < 0.001, #### P < 0.0001 compared with the control group.

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