USF2 Antibody - #AF0291

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产品: USF2 抗体
货号: AF0291
描述: Rabbit polyclonal antibody to USF2
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 40kDa; 37kD(Calculated).
蛋白号: Q15853
RRID: AB_2833457

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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MS Report
HPLC Report
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说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Zebrafish(86%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(93%), Xenopus(93%)
克隆:
Polyclonal
特异性:
USF2 Antibody detects endogenous levels of total USF2.
RRID:
AB_2833457
引用格式: Affinity Biosciences Cat# AF0291, RRID:AB_2833457.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

bHLHb12; Class B basic helix-loop-helix protein 12; FIP; FOS interacting protein; FOS-interacting protein; Major late transcription factor 2; Upstream stimulatory factor 2; Upstream transcription factor 2; USF 2; Usf2; USF2_HUMAN;

抗原和靶标

免疫原:
Uniprot:

Q15853(USF2_HUMAN) >>Visit HPA database.

基因/基因ID:
USF2(7392)
表达:
Q15853 USF2_HUMAN:

Ubiquitous.

描述:
USF2 Transcription factor that binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') that is found in a variety of viral and cellular promoters. Interacts with MAF. Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as an homodimer or a heterodimer (USF1/USF2).
序列:
MDMLDPGLDPAASATAAAAASHDKGPEAEEGVELQEGGDGPGAEEQTAVAITSVQQAAFGDHNIQYQFRTETNGGQVTYRVVQVTDGQLDGQGDTAGAVSVVSTAAFAGGQQAVTQVGVDGAAQRPGPAAASVPPGPAAPFPLAVIQNPFSNGGSPAAEAVSGEARFAYFPASSVGDTTAVSVQTTDQSLQAGGQFYVMMTPQDVLQTGTQRTIAPRTHPYSPKIDGTRTPRDERRRAQHNEVERRRRDKINNWIVQLSKIIPDCNADNSKTGASKGGILSKACDYIRELRQTNQRMQETFKEAERLQMDNELLRQQIEELKNENALLRAQLQQHNLEMVGEGTRQ

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
93
Chicken
93
Zebrafish
86
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q15853 作为底物

Site PTM Type Enzyme
S155 Phosphorylation P49841 (GSK3B)
Y221 Phosphorylation
S222 Phosphorylation
K224 Ubiquitination
T228 Phosphorylation
T230 Phosphorylation P49841 (GSK3B)
K250 Ubiquitination
K271 Ubiquitination
T272 Phosphorylation
S281 Phosphorylation
T293 Phosphorylation
K302 Ubiquitination
K322 Ubiquitination

研究背景

功能:

Transcription factor that binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') that is found in a variety of viral and cellular promoters.

细胞定位:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Ubiquitous.

亚基结构:

Interacts with MAF (By similarity). Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a homodimer or a heterodimer (USF1/USF2). In vivo, the USF1/USF2A heterodimer represents over 66% of the usf binding activity whereas the USF1 and USF2A homodimers represent less than 10%. The USF1/USF2B heterodimer accounted for almost 15% in some cell.

文献引用

1). S100A8 promotes epithelial‐mesenchymal transition and metastasis under TGF‐β/USF2 axis in colorectal cancer. Cancer Communications, 2021 (PubMed: 33389821) [IF=16.2]

Application: IHC    Species: human    Sample: CRC tissues

FIGURE 4| USF2 promotes migration and invasion in CRC cells and is associated with cancer progression and poor survival in CRC patients. A.USF2 overexpression enhanced cell migration and invasion in SW480 and HCT8 cells. B. USF2 knockdown inhibited cell migration and invasion in DLD1 and HT29 cells. C. Representative IHC images showed the coexpression of USF2 cytoplasmic staining (right panel) and S100A8 (left panel) in CRC tissues.

Application: WB    Species: human    Sample: HT29 cells

FIGURE 6| TGF-β promoted EMT and cell mobility by up-regulating USF2/S100A8.F-G. Compared to the control, TGF-β did not induce EMT in USF2 knocked-down DLD1 (F) and HT29 (G) cells. The WB results were repeated three times. The gray values are shown in mean ± SD and analyzed by Student’s t-test. *, P <0.05; **, P <0.01; ***, P <0.001; ns, not significant

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