PKC theta Antibody - #AF6394

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产品: PKC theta 抗体
货号: AF6394
描述: Rabbit polyclonal antibody to PKC theta
应用: WB IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog
蛋白号: Q04759
RRID: AB_2835229

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
PKC theta Antibody detects endogenous levels of total PKC theta.
RRID:
AB_2835229
引用格式: Affinity Biosciences Cat# AF6394, RRID:AB_2835229.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

nPKC-theta; PRKCT; Protein kinase C theta; Protein kinase C theta type;

抗原和靶标

免疫原:

A synthesized peptide derived from human PKC theta, corresponding to a region within C-terminal amino acids.

基因/基因ID:
PRKCQ(5588)
描述:
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters.

研究领域

· Cellular Processes > Transport and catabolism > Autophagy - animal.   (View pathway)

· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway.   (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Human Diseases > Infectious diseases: Viral > Measles.

· Organismal Systems > Circulatory system > Vascular smooth muscle contraction.   (View pathway)

· Organismal Systems > Immune system > Th1 and Th2 cell differentiation.   (View pathway)

· Organismal Systems > Immune system > Th17 cell differentiation.   (View pathway)

· Organismal Systems > Immune system > T cell receptor signaling pathway.   (View pathway)

· Organismal Systems > Sensory system > Inflammatory mediator regulation of TRP channels.   (View pathway)

· Organismal Systems > Endocrine system > Adipocytokine signaling pathway.

文献引用

1). Exogenous Thymosin Beta 4 Suppresses IPF-Lung Cancer in Mice: Possibly Associated with Its Inhibitory Effect on the JAK2/STAT3 Signaling Pathway. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2023 (PubMed: 36835236) [IF=5.6]

Application: WB    Species: Mouse    Sample: Mlg cells

Figure 5. Exogenous rhTβ4 inhibits pulmonary fibrosis and lung cancer via the IL6/JAK2/STAT3 signaling pathway. (A) The luciferase activity in the STAT3-SIE-luc-293 cell lysates treated with IL-6 (70 ng/mL) and rhTβ4. (B) The viability of rhTβ4-treated HEK-293 cells. (C) Western blot assay of JAK2, p-JAK2, STAT3 and p-STAT3 expression in rhTβ4-treated A549 cells. (D) Western blot assay of JAK2, p-JAK2, STAT3 and p-STAT3 expression in rhTβ4-treated Mlg cells. (E,F) Immunohistochemical staining analysis of p-JAK2 and p-STAT3 in mice. Scale bar = 50 μm. Data are shown as the mean ± SD.
2). Combining metabolomics and network pharmacology to investigate the protective effect of Jiawei Xinglou Chengqi Granules in ischemic stroke. Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas, 2024 (PubMed: 38958365) [IF=1.9]

Application: WB    Species: Rat    Sample:

Figure 6 Results of the effect of Jiawei Xinglou Chengqi Granule (JXCG) on neural function in middle cerebral artery occlusion (MCAO) rats. A, Results of TTC staining of brain slices from each group. B, Results of cerebral infarct area measurement. C, Results of Zea-Longa motor nerve function score. D, Results of HE staining (scale bar 100 μm). E-H, Western blot detection of PRKCE, PRKCH, PRKCQ protein expression. Data are reported as means and SD. **P

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