Caveolin 1 Antibody - #AF6386

Fig. 2: SLC15A3 deficiency protects mice from BLM-induced pulmonary fibrosis. A Appearance and morphology of lung tissues. B Assessment of lung index (lung wet weight (mg)/body weight (g)). C Changes of mouse body weight within 21 days after BLM (2 mg/kg) instillation. D Survival of WT and KO mice within 21 days after high dose (3 mg/kg) of BLM treatment, followed by Log-rank (Mantel-Cox) test. E Col1a1, Tgfb1, Fn1 mRNA expression in mouse lung. F, G Representative results and quantification of Col1, E-Cad, Arg1, and Cav-1 protein expression in mouse lung (n = 3, GAPDH served as a loading control. H H&E staining (top) and Masson staining (bottom) of mice lung sections. Scale bars: 50 μm (top left). I Extent of pulmonary fibrosis was quantified by Ashcroft score. J Lung hydroxyproline content. Data are represented as means ± SD, n = 6 for (B), (E), and (J). One-way ANOVA followed by Tukey’s multiple comparisons test for (B), (E), (G), (I), and (J), *P 

Figure 1. Changes in oxidative stress indicators, expression of caveolin 1 (CAV1) and autophagy-related proteins in mammary gland tissue of healthy cows (n = 15) and cows with clinical ketosis (n = 15). (A) Abundance of glutathione peroxidase (GSH-Px) and (B) catalase (CAT), and (C) activities of superoxide dismutase (SOD) and (D) malondialdehyde (MDA) in mammary gland tissue. (E) Representative blots of CAV1, beclin 1, autophagy-related gene 5 (ATG5), sequestosome-1 (SQSTM1, also called p62), and microtubule-associated protein 1 light chain (LC3). (F) Quantification of CAV1, beclin 1, ATG5, p62, and LC3, respectively. (G) mRNA abundance of CAV1, BECN1, ATG5, SQSTM1, and MAP1LC3. Statistical differences were analyzed by paired t-tests, and data were expressed as mean ± SEM. *P < 0.05, **P < 0.01, and ***P < 0.001 compared with healthy group.

Fig. 5|The diferentially expressed proteins were validated by Western Blot. Compared with adjacent normal tissues, the protein expression of PTMA, PAK2, PPP1CA, HMGB2 were up-regulated (a, b), and the protein expression of Caveolin, Integrin beta-1, Collagen alpha-2(VI), Leiomodin-1, Vinculin were down-regulated in ESCC tissues from four pairs of samples (c, d). Representative immunoblot images (a, c) and histograms (mean ± SD; b, d).The experiments were repeated at least three times, N represented normal tissues and T represented tumor tissues