LIMK1 Antibody - #AF6345

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产品: LIMK1 抗体
货号: AF6345
描述: Rabbit polyclonal antibody to LIMK1
应用: WB IF/ICC
文献验证: WB
反应: Human, Mouse, Rat, Monkey
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
蛋白号: P53667
RRID: AB_2835201

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat, Monkey
克隆:
Polyclonal
特异性:
LIMK1 Antibody detects endogenous levels of total LIMK1.
RRID:
AB_2835201
引用格式: Affinity Biosciences Cat# AF6345, RRID:AB_2835201.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

EC 2.7.11.1; LIM domain kinase 1; LIM motif-containing protein kinase; LIMK; LIMK-1; limk1; LIMK1_HUMAN;

抗原和靶标

免疫原:

A synthesized peptide derived from human LIMK1, corresponding to a region within the internal amino acids.

基因/基因ID:
LIMK1(3984)
描述:
There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is likely to be a component of an intracellular signaling pathway and may be involved in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome. Two splice variant have been identified.

研究领域

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Organismal Systems > Development > Axon guidance.   (View pathway)

· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis.   (View pathway)

文献引用

1). The p75 neurotrophin receptor as a novel intermediate in L-dopa-induced dyskinesia in experimental Parkinson's disease. Experimental Neurology, 2021 (PubMed: 33971218) [IF=4.6]

Application: WB    Species: Rat    Sample: striatal tissue

Fig. 3. TH, ΔFosB, p75NTR and Iba1 expression in sham, PD and LID rats. (A-E) TH, ΔFosB, p75NTR, ROCK2, total and phosphor-LIMK1, total and phosphor-Cofilin, and Iba1 protein levels determined by WB from ipsilateral striatal extracts obtained from sham, PD and LID rats and actin as loading control. Data are mean ± SEM (4 striata/group) (One-way ANOVA followed by Sidak’s multiple comparisons test). *P < 0.05, ***P < 0.001 vs sham lesioned group lesioned striatal tissue; ## P < 0.05,### P < 0.001 vs 6-OHDA lesioned group lesioned striatal tissue.) Abbreviations: PD, Parkinson’s disease; LID, L-dopa-induced dyskinesia; p75NTR, p75 neurotrophic receptor; WB, Western Blot; TH, Tyrosine Hydroxylase.
2). Chaperonin-Containing TCP1 Subunit 5 Protects Against the Effect of Mer Receptor Tyrosine Kinase Knockdown in Retinal Pigment Epithelial Cells by Interacting With Filamentous Actin and Activating the LIM-Kinase 1/Cofilin Pathway. Frontiers in medicine, 2022 (PubMed: 35492354) [IF=3.9]

Application: WB    Species: human    Sample: HsRPE cells

Figure 5. CCT5 rescued HsRPE cells with MERTK-associated RP via the LIMK1/cofilin, but not the SSH1/cofilin, pathway. (A) HsRPE cells were treated with or without CCT5 siRNA or CCT5-Le after exposure to NC or MERTK siRNA for 48 h, and the cofilin and p-cofilin expression was analyzed by Western blotting. (B) HsRPE cells were treated the same as in (A), and the expression of LIMK1, TESK1, and SSH1 was analyzed by Western blotting. *P < 0.05; **P < 0.01; *** P < 0.001.

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