产品: Tubulin gamma 抗体
货号: AF0285
描述: Rabbit polyclonal antibody to Tubulin gamma
应用: WB IHC
反应: Human, Mouse, Rat
预测: Zebrafish
分子量: 51kDa; 51kD(Calculated).
蛋白号: P23258
RRID: AB_2833453

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Zebrafish(88%)
克隆:
Polyclonal
特异性:
Tubulin gamma Antibody detects endogenous levels of total Tubulin gamma.
RRID:
AB_2833453
引用格式: Affinity Biosciences Cat# AF0285, RRID:AB_2833453.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Gamma 1 tubulin; Gamma 2 tubulin; Gamma Tubulin 1; Gamma Tubulin 2; Gamma tubulin complex component 1; Gamma-2-tubulin; GCP 1; GCP1; MGC131994; TBG2_HUMAN; TUBG; TUBG1; TUBG2; TUBGCP1; Tubulin gamma 1 chain; Tubulin gamma 2 chain; Tubulin gamma complex-associated protein 1; Tubulin gamma-2 chain; tubulin, gamma 1; tubulin, gamma 2; tubulin, gamma polypeptide; Xgam;

抗原和靶标

免疫原:

A synthesized peptide derived from human Tubulin gamma, corresponding to a region within N-terminal amino acids.

Uniprot:
基因/基因ID:
描述:
TUBG2 Tubulin is the major constituent of microtubules. Gamma tubulin is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta tubulin minus-end nucleation, centrosome duplication and spindle formation (By similarity).
序列:
MPREIITLQLGQCGNQIGFEFWKQLCAEHGISPEGIVEEFATEGTDRKDVFFYQADDEHYIPRAVLLDLEPRVIHSILNSPYAKLYNPENIYLSEHGGGAGNNWASGFSQGEKIHEDIFDIIDREADGSDSLEGFVLCHSIAGGTGSGLGSYLLERLNDRYPKKLVQTYSVFPNQDEMSDVVVQPYNSLLTLKRLTQNADCVVVLDNTALNRIATDRLHIQNPSFSQINQLVSTIMSASTTTLRYPGYMNNDLIGLIASLIPTPRLHFLMTGYTPLTTDQSVASVRKTTVLDVMRRLLQPKNVMVSTGRDRQTNHCYIAILNIIQGEVDPTQVHKSLQRIRERKLANFIPWGPASIQVALSRKSPYLPSAHRVSGLMMANHTSISSLFERTCRQYDKLRKREAFLEQFRKEDMFKDNFDEMDTSREIVQQLIDEYHAATRPDYISWGTQEQ

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Zebrafish
88
Pig
75
Horse
75
Bovine
75
Sheep
75
Xenopus
75
Rabbit
75
Dog
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P23258 作为底物

Site PTM Type Enzyme
S32 Phosphorylation
K48 Ubiquitination
S80 Phosphorylation P24941 (CDK2)
K84 Ubiquitination
K113 Ubiquitination
S129 Phosphorylation
S131 Phosphorylation Q8TDC3-2 (BRSK1)
K164 Ubiquitination
K193 Ubiquitination
S284 Phosphorylation
K287 Ubiquitination
R295 Methylation
K301 Ubiquitination
S306 Phosphorylation
T307 Phosphorylation
K335 Ubiquitination
S336 Phosphorylation
K344 Ubiquitination
K363 Ubiquitination
S364 Phosphorylation
S385 Phosphorylation Q8TDC3 (BRSK1)
K397 Acetylation
K400 Acetylation
K400 Ubiquitination
K410 Ubiquitination
K415 Ubiquitination
T423 Phosphorylation
S424 Phosphorylation

研究背景

功能:

Tubulin is the major constituent of microtubules. The gamma chain is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta chain minus-end nucleation, centrosome duplication and spindle formation.

翻译修饰:

Phosphorylation at Ser-131 by BRSK1 regulates centrosome duplication, possibly by mediating relocation of gamma-tubulin and its associated proteins from the cytoplasm to the centrosome.

细胞定位:

Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Interacts with TUBGCP2 and TUBGCP3. Interacts with B9D2 (By similarity). Interacts with CDK5RAP2; the interaction is leading to centrosomal localization of TUBG1 and CDK5RAP2. Interacts with PIFO. Interacts with SAS6 and NUP62 at the centrosome.

蛋白家族:

Belongs to the tubulin family.

研究领域

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

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