C/EBP alpha Antibody - #AF6333

Fig. 3 Stably overexpressing zebrafish bmp8a or mouse Bmp8a inhibits adipogenesis. a Protocol for effective differentiation of 3T3-L1 cells into adipocytes. b The mRNA expression pattern of mouse Bmp8a, Pparγ and C/ebpα during 3T3-L1 cells differentiated into adipocytes (n = 3). c, d Immunoblot analysis of mouse BMP8A protein expression in 3T3-L1 cells (Mock), stably overexpressed empty plasmid in 3T3-L1 cells (LV-ZsGreen1), stably overexpressed mouse Bmp8a in 3T3-L1 cells (LV-Bmp8a), 3T3-L1 cells infected with scramble shRNA lentivirus (LV-shRNA-scrambled), and knockdown mouse Bmp8a in 3T3-L1 cells (shRNA-Bmp8a#1 and shRNA-Bmp8a#2). BMP8A protein expression levels were quantified by ImageJ software and normalized to the amount of β-actin (d, n = 3). e, f After induction of adipogenic differentiation, differentiated 3T3-L1 adipocytes (Mock, LV-ZsGreen1, LV-bmp8a, and LV-Bmp8a) were stained with Oil Red O and subjected to OD492 quantifications (n = 3). Scale bar = 20 µm. g–j On the day after induction as indicated, expressions of adipogenic genes (Cebpα, Pparγ, and Fasn) were examined at the mRNA level by qPCR (n = 3). k–m On the day after induction, as indicated, the protein levels of PPARγ and C/EBPα detected by Immunoblot. Protein expression levels were quantified using ImageJ software and normalized to the amount of β-actin (l, m, n = 3). Data were representative of at least three independent experiments. Data were analyzed by One-way ANOVA and presented as mean ± SD

Figure 5. |miR‑139‑5p modulated HemSCs differentiation into adipocytes through IGF‑1/IGF‑1R. (A) Adipogenic differentiation of HemSCs was determined by oil red o staining to visualize intracellular lipid droplet accumulation. (B) Oil red o‑stained cells were quantified using ImageJ software. (C) Western blot analysis demonstrated the expression levels of (D) PPAR‑γ, (E) C/EBPα and (F) C/EBPβ in HemSCs transfected with miR‑139‑5p mimics or inhibitor and treated with or without 100 ng/ml IGF‑1.

Fig. 5.| Adipo-8 ameliorated fat deposition through interaction with APMAP: (C) Expression of fat metabolism related proteins: aP2, PPAR-γ, and C/EBP-α down-regulated by adipo-8 in APMAP-NC adipocytes, but not in APMAP-silent adipocytes. Control, library and adipo-8(0.01 μg/g/day for 21 days) in high fat fed mice: adipo-8 reduced body weight of HFD fed mice (P < 0.05) (D), decrease adipocytes volume (E), lowered TG(*P < 0.05) (F), but not TC (**P > 0.05) (G)without liver or renal function damage (**P > 0.05) (H).

Figure 3. WSTLZT-induced exosomes regulate osteoblastic and adipogenic differentiation of BMSC in the co-culture system of BMSC and adipocyte-derived exosomes. (A) ALP staining, Alizarin red staining, Oil red O staining, scale bar = 100 μm; (B) the protein levels of osteogenic markers RUNX2, Osterix and adipogenic markers CEBP-α, PPARγ2 were measured by western blot. (C) Protein expression of RUNX2, Osterix, CEBP-α and PPARγ2 was analysed relative to those in control. Mean ± SEM (n = 3). Compared with the control group, **p