ZAP70 Antibody - #AF6312

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产品: ZAP70 抗体
货号: AF6312
描述: Rabbit polyclonal antibody to ZAP70
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
蛋白号: P43403
RRID: AB_2835171

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
ZAP70 Antibody detects endogenous levels of total ZAP70.
RRID:
AB_2835171
引用格式: Affinity Biosciences Cat# AF6312, RRID:AB_2835171.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

70 kDa zeta associated protein; 70 kDa zeta-associated protein; EC 2.7.10.2; FLJ17670; FLJ17679; Selective T cell defect; SRK; STD; Syk related tyrosine kinase; Syk-related tyrosine kinase; Truncated ZAP kinase; Tyrosine protein kinase ZAP70; Tyrosine-protein kinase ZAP-70; TZK; ZAP 70; ZAP70; ZAP70_HUMAN; Zeta chain associated protein kinase 70kD; Zeta chain associated protein kinase 70kDa; Zeta chain associated protein kinase 70kDa isoform 1; Zeta chain associated protein kinase 70kDa isoform 2; Zeta chain TCR associated protein kinase 70kD; Zeta chain TCR associated protein kinase 70kDa;

抗原和靶标

免疫原:

A synthesized peptide derived from human ZAP70, corresponding to a region within the internal amino acids.

基因/基因ID:
ZAP70(7535)
描述:
ZAP70 (human) a tyrosine kinase of the Syk family. Associates with the T-cell antigen receptor zeta-chain after TCR stimulation. Phosphorylated by Src-family kinases following antigen receptor activation. Plays a role in lymphocyte activation.

研究领域

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway.   (View pathway)

· Human Diseases > Immune diseases > Primary immunodeficiency.

· Organismal Systems > Immune system > Natural killer cell mediated cytotoxicity.   (View pathway)

· Organismal Systems > Immune system > Th1 and Th2 cell differentiation.   (View pathway)

· Organismal Systems > Immune system > Th17 cell differentiation.   (View pathway)

· Organismal Systems > Immune system > T cell receptor signaling pathway.   (View pathway)

文献引用

1). Endometrial regenerative cells with galectin-9 high-expression attenuate experimental autoimmune hepatitis. Stem cell research & therapy, 2021 (PubMed: 34654474) [IF=7.5]

Application: WB    Species: Mouse    Sample:

Fig. 7 Inhibition of T cell proliferation by Gal-9 high-expressing ERCs is associated with Lck/ZAP70/LAT pathway. The naïve CD4+ T cells in the mouse spleen were collected by magnetic bead, and then the harvested CD4+ T cells were co-cultured with unmodified ERCs or Gal-9 high-expressing ERCs for 6 days. A CD4+ T cell proliferation was determined by CCK-8 assay. B The protein expressions and phosphorylation form protein expressions of Lck, ZAP-70, LAT were detected by western blot. The differences between groups were calculated by using ANOVA. *p 
2). An IgD-Fc-Ig fusion protein restrains the activation of T and B cells by inhibiting IgD-IgDR-Lck signaling in rheumatoid arthritis. Acta pharmacologica Sinica, 2022 (PubMed: 33864023) [IF=6.9]
3). Regulation of T Cell Activities in Rheumatoid Arthritis by the Novel Fusion Protein IgD-Fc-Ig. Frontiers in Immunology, 2020 (PubMed: 32499775) [IF=5.7]

Application: WB    Species: mouse    Sample: spleen

FIGURE 9 | Effects of IgD-Fc-Ig (DG) on the protein expression of Lck, p-Lck, ZAP70 and p-ZAP70. (A–C) Western blot analysis of p-Lck and p-ZAP70 expression in PBMCs of RA patients, PBMCs were treated with IgD (3µg/mL) and IgD-Fc-Ig (1, 3, 10µg/mL) for 48h and lysed. (D–F) Western blot analysis of Lck, p-Lck, ZAP70 and p-ZAP70 expression in CIA mice. Mice spleens from each group were isolated and homogenized in lysis buffer.
4). New Findings and Possibilities: Glycyrrhizic Acid Target Klrc2 to Protect Cfs Rats. , 2024

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