WNK1 Antibody - #AF6483

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产品: WNK1 抗体
货号: AF6483
描述: Rabbit polyclonal antibody to WNK1
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Bovine, Sheep, Rabbit, Dog, Chicken
蛋白号: Q9H4A3
RRID: AB_2835163

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MSDS
说明书
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
WNK1 Antibody detects endogenous levels of total WNK1.
RRID:
AB_2835163
引用格式: Affinity Biosciences Cat# AF6483, RRID:AB_2835163.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Erythrocyte 65 kDa protein; HSAN2; HSN2; hWNK1; KDP; KIAA0344; Kinase deficient protein; MGC163339; MGC163341; p65; PPP1R167; PRKWNK1; Prostate derived sterile 20 like kinase; Protein kinase lysine deficient 1; Protein kinase lysine-deficient 1; Protein kinase with no lysine 1; Protein phosphatase 1, regulatory subunit 167; PSK; Serine/threonine protein kinase WNK1 1; Serine/threonine protein kinase WNK1 2; Serine/threonine protein kinase WNK1; Serine/threonine-protein kinase WNK1; With no K; WNK lysine deficient protein kinase 1; WNK lysine deficient protein kinase 1 isoform; WNK1; WNK1_HUMAN;

抗原和靶标

免疫原:

A synthesized peptide derived from human WNK1, corresponding to a region within N-terminal amino acids.

基因/基因ID:
WNK1(65125)
描述:
WNK1 a serine-threonine protein kinase that controls sodium and chloride ion transport. May regulate the activity of the thiazide-sensitive Na-Cl cotransporter SLC12A3 by phosphorylation. May also play a role in actin cytoskeletal reorganization.

文献引用

1). MiR-524-5p inhibits angiogenesis through targeting WNK1 in colon cancer cells. AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY, 2020 (PubMed: 32174132) [IF=3.9]

Application: WB    Species: human    Sample: HT29 cells

Fig. 6.| WNK1 silence inhibited angiogenesis in vitro. (A) The relative mRNA and protein levels of WNK1 in HT29 cells.
2). Regulatory role of PI3K/Akt/WNK1 signal pathway in mouse model of bone cancer pain. Scientific reports, 2023 (PubMed: 37652923) [IF=3.8]

Application: WB    Species: Mouse    Sample:

Figure 6 (A)、(C)、(E) Western blotting to detect the expression of PI3K, p-PI3K, Akt, p-Akt, WNK1 and p-WNK1 protein. (B)、(D)、(F) The optical density of protein bands detected by Western blotting was statistically quantified by IPP 6.0 software; (B) Protein expression of PI3K and p-PI3K; (D) Protein expression of Akt and p-Akt; (F) Protein expression of WNK1 and p-WNK1. N: Normal group, BCP: Bone cancer pain group, Akt-i: Akt inhibitor group. n = 3; *P 

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