HNF4 alpha Antibody - #AF6297

>>买2送1 进行中>>
>>赠JD卡 进行中>>
(2)   (2)  
产品: HNF4 alpha 抗体
货号: AF6297
描述: Rabbit polyclonal antibody to HNF4 alpha
应用: WB IF/ICC
文献验证: WB, IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
蛋白号: P41235
RRID: AB_2835147

浏览相似产品>>

   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

联系销售
相关下载
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
HNF4 alpha Antibody detects endogenous levels of total HNF4 alpha.
RRID:
AB_2835147
引用格式: Affinity Biosciences Cat# AF6297, RRID:AB_2835147.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

FLJ39654; FRTS4; Hepatic nuclear factor 4 alpha; Hepatocyte nuclear factor 4 alpha; Hepatocyte nuclear factor 4; Hepatocyte nuclear factor 4-alpha; HNF 4 alpha; HNF 4; HNF-4-alpha; HNF4; HNF4A; HNF4A_HUMAN; HNF4a7; HNF4a8; HNF4a9; Hnf4alpha; HNF4alpha10/11/12; MODY 1; MODY; MODY1; NR2A1; NR2A21; Nuclear receptor subfamily 2 group A member 1; OTTHUMP00000031060; OTTHUMP00000031062; TCF 14; TCF; TCF-14; TCF14; Tcf4; Transcription factor 14, hepatic nuclear factor; Transcription factor 14; Transcription factor HNF 4; Transcription factor HNF-4; Transcription factor HNF4;

抗原和靶标

免疫原:

A synthesized peptide derived from human HNF4 alpha, corresponding to a region within the internal amino acids.

基因/基因ID:
HNF4A(3172)
描述:
The protein encoded by this gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes.

研究领域

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Maturity onset diabetes of the young.

文献引用

1). Naringenin in Si-Ni-San formula inhibits chronic psychological stress-induced breast cancer growth and metastasis by modulating estrogen metabolism through FXR/EST pathway. Journal of advanced research, 2023 (PubMed: 35718080) [IF=11.4]

Application: WB    Species: Mouse    Sample:

Fig. 5. SNS activates HNF4α-mediated EST transcription via inhibiting cholic acid-induced FXR expression. (A-B) Immunoblotting and immunofluorescence analyses of FXR and EST expression by SNS in a time-dependent manner. (C) EST mRNA expression was examined by quantitative PCR. (D-E) The influence of SNS on HNF4α expression in a time-dependent manner. (F) FXR overexpression inhibited HNF4α and EST activation, which was rescued by SNS treatment. (G-H) Immunoblotting and immunofluorescence showed that FXR overexpression inhibited nuclear HNF4α transportation, and SNS reactivate HNF4α nuclear translocation. (I) Transcriptional activity of EST promoter after FXR overexpression or SNS treatment. (J) The binding sites between HNF4α and EST promoters affected by FXR overexpression or SNS treatment were detected by ChIP assay. Data are represented as mean ± SD, n = 3, *P < 0.05, #P < 0.01.

Application: IF/ICC    Species: Mouse    Sample:

Fig. 5. SNS activates HNF4α-mediated EST transcription via inhibiting cholic acid-induced FXR expression. (A-B) Immunoblotting and immunofluorescence analyses of FXR and EST expression by SNS in a time-dependent manner. (C) EST mRNA expression was examined by quantitative PCR. (D-E) The influence of SNS on HNF4α expression in a time-dependent manner. (F) FXR overexpression inhibited HNF4α and EST activation, which was rescued by SNS treatment. (G-H) Immunoblotting and immunofluorescence showed that FXR overexpression inhibited nuclear HNF4α transportation, and SNS reactivate HNF4α nuclear translocation. (I) Transcriptional activity of EST promoter after FXR overexpression or SNS treatment. (J) The binding sites between HNF4α and EST promoters affected by FXR overexpression or SNS treatment were detected by ChIP assay. Data are represented as mean ± SD, n = 3, *P < 0.05, #P < 0.01.
2). Structure-based identification of HNF4α agonists: Rosmarinic acid as a promising candidate for NAFLD treatment. Computational and structural biotechnology journal, 2024 (PubMed: 39850659) [IF=4.4]

限制条款

产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）

产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。 

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.