Calcium Sensing Receptor Antibody - #AF6296

Figure 6 Cyclo(pro-trp) can alleviate the severity of hyperparathyroidism. A) Molecular structure of cyclo(pro-trp). B) Serum CR and BUN levels. No damage to renal function. C) Serum ALT, AST, ALP, TBIL, and DBIL levels. No damage to liver function. D) iPTH levels in different groups of mice. Cyclo(pro-trp) can reduce iPTH levels in CKD mice. E) Calcium contents of intestine tissue supernatant-treated mice. Cyclo(pro-trp) can increase the intestinal calcium content before and after CKD modeling in mice. F) mRNA expression levels of CaSR, TPCN1, CACNA1A, SLC8A1, and TRPV4 in intestine tissue of recipient mice. CaSR and TRPV4 significantly increased in cyclo(pro-trp)-treated mice. G) Analysis of parathyroid area scores. The control mice had significantly increased parathyroid tissue hyperplasia and area score, with more severe hyperparathyroidism. H) H&E staining of the parathyroid tissue of recipient mice. Scale bar = 100 µm. I) Co-localization of CaSR and TRPV4 in intestinal epithelial cells (IECs) detected via immunofluorescence staining. Scale bar = 100 µm. J) Fluorescence quantitation in IECs of recipient mice. Significant expression of CaSR and TRPV4 in cyclo(pro-trp)-treated mice. K) mRNA expression levels of CaSR, TPCN1, CACNA1A, SLC8A1, and TRPV4 in the IECs of recipient mice. The expression levels of CaSR and TRPV4 significantly increased in cyclo(pro-trp)-treated mice. L) Representative Ca2+ imaging of MODE-K cells. Scale bar = 20 µm. M) Time-lapse averaged Ca2+ traces of MODE-K cells followed by the administration of cyclo(pro-trp) (100 µM) in the 10th second after boarding the machine. N) ΔF/F0 peak amplitude. The cyclo(pro-tp) group had significantly higher values than the control group. By unpaired two-tailed Student's t-test. O) Accumulated bound calcium content in MODE-K cells. P) mRNA expression levels of CaSR and TRPV4 in MODE-K cells. The values are presented as mean ± SD in Figure 6B–F,K (n = 6–8), Figure 6G,H (n = 4), Figure 6I,J (n = 3), and Figure 6L,P (n = 6); *p < 0.05; **p < 0.01; ***p < 0.001 compared with the control group.

Fig. 6. |Effect of SNS on CaSR expression in the CA1 of HIP and PFC of rats. (A–B): Quantitative analysis of IHC in the hippocampal CA1 and PFC regions. (C–D)Representative IHC figures of CA1 and PFC, scale bar = 50 μm

Fig. 6. |Effect of SNS on CaSR expression in the CA1 of HIP and PFC of rats. (A–B): Quantitative analysis of IHC in the hippocampal CA1 and PFC regions. (C–D)Representative IHC figures of CA1 and PFC, scale bar = 50 μm.(E-F):Representative immunoblots for CaSR and tubulin in the HIP and PFC regions; Relative protein levels of CaSR in the HIP(E) and PFC(F) of rats in each group. All data are expressed as mean ± S.E.M.