AFfirm™ HO-1 Mouse Monoclonal Antibody - #BF8020

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产品: HO-1 小鼠 单克隆 抗体
货号: BF8020
描述: Mouse monoclonal antibody to HO-1
应用: WB IHC IF/ICC
文献验证: WB, IHC, IF/ICC
反应: Human, Mouse
蛋白号: P09601

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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说明书
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Mouse
应用:
WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse
克隆:
Monoclonal [AFfirm8020]
特异性:
HO-1 Antibody detects endogenous levels of total HO-1.
偶联:
Unconjugated.
纯化:
Affinity-chromatography.
保存:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

32 kD; bK286B10; D8Wsu38e; heat shock protein 32 kD; heat shock protein 32kD; Heat shock protein; Heme oxygenase (decycling) 1; Heme oxygenase 1; Hemox; HMOX 1; Hmox; Hmox1; HMOX1_HUMAN; HO 1; HO; HO-1; HO1; Hsp32;

抗原和靶标

免疫原:

A synthesized peptide derived from human HO-1.

基因/基因ID:
HMOX1(3162)

研究领域

· Cellular Processes > Cell growth and death > Ferroptosis.   (View pathway)

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma.   (View pathway)

· Metabolism > Metabolism of cofactors and vitamins > Porphyrin and chlorophyll metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Digestive system > Mineral absorption.

文献引用

1). Natural Linoleic Acid from Marine Fungus Eutypella sp. F0219 Blocks KEAP1/NRF2 Interaction and Ameliorates MASLD by Targeting FABP4. Free radical biology & medicine, 2024 (PubMed: 39299527) [IF=7.1]
2). The ferroptosis of sertoli cells inducing blood-testis barrier damage is produced by oxidative stress in cryptorchidism. Free radical biology & medicine, 2025 (PubMed: 40032029) [IF=7.1]
3). Dihydroartemisinin inhibits galectin-1-induced ferroptosis resistance and peritoneal metastasis of gastric cancer via the Nrf2-HO-1 pathway. Phytomedicine : international journal of phytotherapy and phytopharmacology, 2025 (PubMed: 41166905) [IF=6.7]

Application: WB    Species: human    Sample: HGC-27 cells

Fig. 7. Nrf2 inhibition attenuates the ferroptosis resistance induced by galectin-1 overexpression. Western blot analyses were performed to detect the HO-1 and GPX4 expression levels in SGC-7901(A) and HGC-27 (B)cells with KD-LGALS1or OE-LGALS1 (C, D). Immunofluorescence staining was used to evaluate the expression of Nrf2, HO-1, and GPX4 in SGC-7901 (E) and HGC-27 (F) cells with OE-LGALS1 and treated with ML385 (400 × magnification). (G, H) Quantitative analysis of target protein expression across experimental groups. (I) BODIPY C11 staining was performed to assess lipid ROS levels in GC cells with OE-LGALS1 and treated with ML385 (200 × magnification). (J) Quantitative analysis of the fluorescence intensity of oxidized BODIPY across the groups. WT: Wild Type; NS: not statistically significant

Application: IF/ICC    Species: human    Sample: HGC-27 cells

Fig. 7. Nrf2 inhibition attenuates the ferroptosis resistance induced by galectin-1 overexpression. Western blot analyses were performed to detect the HO-1 and GPX4 expression levels in SGC-7901(A) and HGC-27 (B)cells with KD-LGALS1or OE-LGALS1 (C, D). Immunofluorescence staining was used to evaluate the expression of Nrf2, HO-1, and GPX4 in SGC-7901 (E) and HGC-27 (F) cells with OE-LGALS1 and treated with ML385 (400 × magnification). (G, H) Quantitative analysis of target protein expression across experimental groups. (I) BODIPY C11 staining was performed to assess lipid ROS levels in GC cells with OE-LGALS1 and treated with ML385 (200 × magnification). (J) Quantitative analysis of the fluorescence intensity of oxidized BODIPY across the groups. WT: Wild Type; NS: not statistically significant

Application: IHC    Species: Mouse    Sample:

Fig. 9. Galectin-1 activates the PI3K/Akt/Nrf2/HO-1/GPX4 signal-ing pathway to inhibit ferroptosis and promote GCPM. (A) Schematic illustration of the GCPM animal model. (B) Representative images of H&E staining, iron staining, and IHC for TfR1, FPN1, and GPX4 in the mouse PM model (400 × magnification). (C, D) Statistical analysis of the IOD of iron-stained and the IRSs of TfR1 FPN1 and GPX4 in each group. (E) Representative images of IHC for galectin-1, PI3K, p-PI3K, Akt, p-Akt, Nrf2, and HO-1 in peritoneal nodule tissues from each group (400 × magnification). (F–I) Quantitative analysis of the IRSs of target proteins across the experimental groups. WT: Wild Type; NS: not statistically significant
4). Molybdenum Nanoparticles Alleviate MC903-Induced Atopic Dermatitis-Like Symptoms in Mice by Modulating the ROS-Mediated NF-κB and Nrf2 /HO-1 Signaling Pathways. International journal of nanomedicine, 2024 (PubMed: 39220192) [IF=6.6]
5). Antioxidation and Anti-Inflammatory Activity of Prussian Blue Nanozymes to Alleviate Acetaminophen-Induced Acute Liver Injury. ACS Applied Nano Materials, 2023 [IF=5.9]
6). Promoting spinal cord injury repair by using ZnO@MOFs nanozymes functionalized hydrogel through the ROS microenvironment regulating pathway. Regenerative biomaterials, 2025 (PubMed: 41190134) [IF=5.6]
7). Bisphenol A induced neuronal apoptosis and enhanced autophagy in vitro through Nrf2/HO-1 and Akt/mTOR pathways. Toxicology, 2023 (PubMed: 38006930) [IF=4.8]
8). Effects of Oltipraz on the Glycolipid Metabolism and the Nrf2/HO-1 Pathway in Type 2 Diabetic Mice. Drug design, development and therapy, 2024 (PubMed: 39654602) [IF=4.7]

Application: WB    Species: Mouse    Sample:

Figure 5 OLTI showed an anti-oxidation stress and anti-apoptosis function in T2DM. (A–G) the expression of Nrf2, HO-1, NQO1, Bax, Bcl-2, Caspase-3, and Reg3g in mRNA level. (H–O) the expression of Nrf2, HO-1, NQO1, Bax, Bcl-2, Caspase-3, and Reg3g in protein level. *P
9). ROS Regulate Rotenone-induced SH-SY5Y Dopamine Neuron Death Through Ferroptosis-mediated Autophagy and Apoptosis. Molecular neurobiology, 2025 (PubMed: 40097764) [IF=4.6]
10). Oleanonic acid ameliorates mutant Aβ precursor protein-induced oxidative stress, autophagy deficits, ferroptosis, mitochondrial damage, and ER stress in vitro. Biochimica et biophysica acta. Molecular basis of disease, 2024 (PubMed: 39134286) [IF=4.2]
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