AFfirm™ Nrf2 mouse monoclonal Antibody - #BF8017

Figure 6 WFA upregulates nuclear factor E2-related factor 2 (Nrf2) expression and promotes nuclear translocation of Nrf2 after ICH in vivo and in vitro. (A) Venn analysis of overlapping genes that interact with both WFA and HO-1 in the CTD database and String database. (B) Representative western blot images and (C) quantitative analysis of relative protein level of cytoplasmic Nrf2 and nuclear Nrf2 in the sham, ICH, and WFA (0.1, 1, and 5 μg/kg)-treated groups on day 7 after ICH. (D) Quantification of relative nuclear and cytoplasmic Nrf2 protein levels in the sham, ICH, and WFA (0.1/1/5 μg/kg)-treated groups on day 7 after ICH. (E) Representative western blot images and (F and G) quantification of relative protein expression of cytoplasmic Nrf2, nuclear Nrf2, and ration of nuclear/cytoplasmic Nrf2 in the hemin-induced ICH model with or without WFA treatment. (H) Nrf2 localization was detected by immunofluorescence with an anti-Nrf2 antibody (green fluorescence). Nuclei were stained with DAPI (blue fluorescence). (I) Allocation of green and blue signals in the dual-channel overlay images was analyzed with the ImageJ “Co-localization Finder” plugin, and co-localization of the green and blue signals is shown in white spots. The x-axes indicate the intensities of the green signals from the green channel (Nrf2) and the y-axes from the blue channel (DAPI). For every scatterplot, the intensities are given as the pixel grey values ranging from 0 to 255. Co-localization clusters the pixels from two channels along the diagonal. Pearson’s correlation coefficient (Rr) reflects the intensity distribution relationship between the two channels. Manders overlap coefficient (MOC) reflects the true degree of co-localization of the two channels. The maximal theoretical value for the Rr and MOC is 1.0. Quantification analysis of Nrf2 nuclear location with parameter. (J) Pearson’s correlation coefficient (Rr) and (K) MOC. n = 6 mice per group in B–D; n = 3 per group in E–K. *P < 0.05, **P < 0.01 (one-way analysis of variance followed by Bonferroni correction). Data are presented as the mean ± SEM. HO-1: Heme oxygenase-1; ICH: intracerebral hemorrhage; Nrf2: nuclear factor erythroid-related factor 2; WFA: withaferin A.

Figure 10 BMSC-derived Exosoe−GATA−4 inhibits H/R-induced cardiomyocytes ferroptosis related to keap1/Nrf2 signaling pathway (A) The content of H2S was assessed using Kits assay. (B and C) The expression levels of keap1 and Nrf2 were detected using RT-qPCR (B) and western blot (C). (D) The expression of Nrf2 was detected using IF (scale bar: 20 μm). n = 3, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. Data are represented as mean ± SD.

Figure 10 BMSC-derived Exosoe−GATA−4 inhibits H/R-induced cardiomyocytes ferroptosis related to keap1/Nrf2 signaling pathway (A) The content of H2S was assessed using Kits assay. (B and C) The expression levels of keap1 and Nrf2 were detected using RT-qPCR (B) and western blot (C). (D) The expression of Nrf2 was detected using IF (scale bar: 20 μm). n = 3, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. Data are represented as mean ± SD.

Figure 5 OLTI showed an anti-oxidation stress and anti-apoptosis function in T2DM. (A–G) the expression of Nrf2, HO-1, NQO1, Bax, Bcl-2, Caspase-3, and Reg3g in mRNA level. (H–O) the expression of Nrf2, HO-1, NQO1, Bax, Bcl-2, Caspase-3, and Reg3g in protein level. *P