PPAR gamma Antibody - #AF6284

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产品: PPAR gamma 抗体
货号: AF6284
描述: Rabbit polyclonal antibody to PPAR gamma
应用: WB IHC IF/ICC
文献验证: WB, IHC, IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog
蛋白号: P37231
RRID: AB_2835135

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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说明书
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
PPAR gamma Antibody detects endogenous levels of total PPAR gamma.
RRID:
AB_2835135
引用格式: Affinity Biosciences Cat# AF6284, RRID:AB_2835135.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CIMT1; GLM1; NR1C3; Nuclear receptor subfamily 1 group C member 3; OTTHUMP00000185032; OTTHUMP00000185036; Peroxisome proliferator activated nuclear receptor gamma variant 1; Peroxisome proliferator activated receptor gamma 1; Peroxisome Proliferator Activated Receptor gamma; Peroxisome proliferator-activated receptor gamma; PPAR gamma; PPAR-gamma; PPARG; PPARG_HUMAN; PPARG1; PPARG2; PPARgamma;

抗原和靶标

免疫原:

A synthesized peptide derived from human PPAR gamma, corresponding to a region within the internal amino acids.

基因/基因ID:
PPARG(5468)
描述:
The protein encoded by this gene is a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma.

研究领域

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

· Human Diseases > Cancers: Specific types > Thyroid cancer.   (View pathway)

· Organismal Systems > Endocrine system > PPAR signaling pathway.

· Organismal Systems > Aging > Longevity regulating pathway.   (View pathway)

· Organismal Systems > Development > Osteoclast differentiation.   (View pathway)

文献引用

1). Atomically precise gold nanoclusters as ROS-scavenging clusterzymes to treat high-fat diet-induced obesity. Chemical Engineering Journal, 2024 [IF=13.3]
2). Mucin 17 inhibits the progression of human gastric cancer by limiting inflammatory responses through a MYH9-p53-RhoA regulatory feedback loop. JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH, 2019 (PubMed: 31262330) [IF=11.3]

Application: WB    Species: human    Sample: AGS cells and MKN45 cells

Fig. 5| MUC17 protects GC cells against inflammatory stimulation by regulating the p38 pathway. a MUC17 regulated the expression of pJNK,pERK, and pp38 MAPK in MUC17 knocked-down AGS cells (left) and truncated MUC17 overexpressed MKN45 cells (right).b The effect of the p38 inhibitor SB203580 on the expression of NFκB pathway proteins and G2/M phase arrest markers in MUC17 knocked-down AGS cells (left) and truncated MUC17 overexpressed MKN45 cells (right).
3). Antifibrotic Effects of Tetrahedral Framework Nucleic Acids by Inhibiting Macrophage Polarization and Macrophage-myofibroblast Transition in Bladder Remodeling. Advanced Healthcare Materials, 2023 (PubMed: 36603196) [IF=10.0]
4). Dioscin ameliorates murine ulcerative colitis by regulating macrophage polarization. Pharmacological research, 2021 (PubMed: 34343656) [IF=9.1]
5). Caveolin-1 mitigates the advancement of metabolic dysfunction-associated steatotic liver disease by reducing endoplasmic reticulum stress and pyroptosis through the restoration of cholesterol homeostasis. International journal of biological sciences, 2025 (PubMed: 39781461) [IF=8.2]
6). CircITGB5 regulates the proliferation and adipogenic differentiation of chicken intramuscular preadipocytes through the miR-181b-5p/CPT1A axis. International journal of biological macromolecules, 2024 (PubMed: 39577521) [IF=7.7]
7). Aqueous extract of Phellinus igniarius ameliorates hyperuricemia and renal injury in adenine/potassium oxonate-treated mice. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 2024 (PubMed: 38879892) [IF=6.9]
8). Shikonin ameliorated LPS-induced acute lung injury in mice via modulating MCU-mediated mitochondrial Ca2+ and macrophage polarization. Phytomedicine : international journal of phytotherapy and phytopharmacology, 2024 (PubMed: 39366155) [IF=6.7]
9). Nrf2 Deficiency Exacerbated CLP-Induced Pulmonary Injury and Inflammation through Autophagy- and NF-κB/PPARγ-Mediated Macrophage Polarization. Cells, 2022 (PubMed: 36497185) [IF=6.0]

Application: IF/ICC    Species: Mice    Sample: RAW264.7 cells

Figure 9 Nrf2 overexpression in vitro promotes PPARγ but inhibits NF-κB nuclear translocation; n = 3 experiments per group. (A,B) Immunofluorescence staining of NF-κB and PPARγ in RAW264.7 cells in each group. (C,D) Western blot analyses of Nrf2, NF-κB, and PPARγ levels in both cytoplasm and nucleus. ** p < 0.01, *** p < 0.001.

Application: WB    Species: Mice    Sample: RAW264.7 cells

Figure 9 Nrf2 overexpression in vitro promotes PPARγ but inhibits NF-κB nuclear translocation; n = 3 experiments per group. (A,B) Immunofluorescence staining of NF-κB and PPARγ in RAW264.7 cells in each group. (C,D) Western blot analyses of Nrf2, NF-κB, and PPARγ levels in both cytoplasm and nucleus. ** p < 0.01, *** p < 0.001.
10). PRDX6 Promotes Fatty Acid Oxidation via PLA2-Dependent PPARα Activation in Rats Fed High-Fat Diet. Antioxidants & redox signaling, 2023 (PubMed: 36401357) [IF=5.9]

Application: WB    Species: Rat    Sample: liver tissues

FIG. 3. PRDX6 KO suppresses fatty acid oxidation-related genes in HFD rats. (A) PPARα mRNA in the liver of WT and PRDX6 KO rats following 12 weeks of NCD or HFD feeding was measured by qPCR (n = 7). (B) PPARα protein level was detected by Western blotting in rat liver tissues. (C) Graph showing Western blot quantification of PPARα/GAPDH (n = 6). (D–F) CPT-1α, ACOX1, and CYP4A1 mRNA levels were measured by qPCR in the liver tissues of PRDX6 KO and WT rats with 12-week NCD or HFD feeding (n = 6). (G) CPT-1α and ACOX1 protein levels were detected by Western blotting in rat liver tissues. (H, I) Western blot quantification of CPT-1α/GAPDH and ACOX1/GAPDH (n = 6). (J) CPT-1α protein level in rat liver tissues was detected by IHC. Scale bar = 50 μm. (K) Quantification of CPT-1α IHC (n = 6). All data are presented as mean ± SEM. Data were analyzed by one-way ANOVA followed by Sidak's test. *p 
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