PPAR gamma Antibody - #AF6284

Figure 4 Mitochondrial dysfunction contributes to weakened uterine contractions. A) Schematic diagram of mitochondrial dysfunction caused by obesity and HFD, created with BioRender.com. B–F) Representative line and bar graphs of OCR in PA-induced USMCs, including basal respiration, maximal respiration, respiratory reserve capacity, proton leak, and ATP production (n = 5 for BSA group, n = 9 for PA group; samples pooled from 3 mice per sample). G) The ATP levels in the uterine myometrium of late-pregnant obese mice (n = 6 samples per group, pooled from 3 mice per sample). H) The ATP levels in the PA-treated cells (n = 6 per group). I) The ATP levels in the uterine myometrium of late-pregnant mice with AAV injection, measured using the ATP Assay Kit (n = 13 for AAV-NC group, n = 16 for AAV-FABP4 group; pooled from 3 mice per sample). J,K) Representative images of Rhod-2 AM (red) staining for mitochondrial calcium and Mito-Tracker (green) labeling for mitochondria in USMCs (n = 21 for BSA group, n = 24 for PA group; pooled from 3 mice per sample), scale bar = 20 µm. L,M) Mitochondrial membrane potential analysis using the JC-1 probe (n = 8 in the BSA group and n = 9 in the PA group), scale bar = 20 µm. N,O) Immunofluorescent images of uterine myometrium stained with TOMM20 (green) and nuclei (blue) in BSA- and PA-treated groups (n = 4 per group), scale bar = 10 µm. P,Q) Western blot and quantitative analysis of PGC-1α and PPAR γ expression in uterine smooth muscle tissue from HFD pregnant mice (n = 6 for control group, n = 9 for obesity group; pooled from 3 mice per sample). R,S) Western blot and quantitative analysis of PGC-1α and PPAR γ expression in USMCs treated with BSA or PA (n = 6 per group). All experiments were repeated 2–3 times with consistent results. The values are expressed as the means ± SDs. *p < 0.05, **p < 0.01, and ***p < 0.001 versus the control group.

Fig. 5| MUC17 protects GC cells against inflammatory stimulation by regulating the p38 pathway. a MUC17 regulated the expression of pJNK,pERK, and pp38 MAPK in MUC17 knocked-down AGS cells (left) and truncated MUC17 overexpressed MKN45 cells (right).b The effect of the p38 inhibitor SB203580 on the expression of NFκB pathway proteins and G2/M phase arrest markers in MUC17 knocked-down AGS cells (left) and truncated MUC17 overexpressed MKN45 cells (right).

Figure 9 Nrf2 overexpression in vitro promotes PPARγ but inhibits NF-κB nuclear translocation; n = 3 experiments per group. (A,B) Immunofluorescence staining of NF-κB and PPARγ in RAW264.7 cells in each group. (C,D) Western blot analyses of Nrf2, NF-κB, and PPARγ levels in both cytoplasm and nucleus. ** p < 0.01, *** p < 0.001.

Figure 9 Nrf2 overexpression in vitro promotes PPARγ but inhibits NF-κB nuclear translocation; n = 3 experiments per group. (A,B) Immunofluorescence staining of NF-κB and PPARγ in RAW264.7 cells in each group. (C,D) Western blot analyses of Nrf2, NF-κB, and PPARγ levels in both cytoplasm and nucleus. ** p < 0.01, *** p < 0.001.