VEGFR2 Antibody - #AF6281

Fig. 5 Apatinib downregulated VEGFR2/STAT3/PD-L1 pathway in NSCLC cells and reduced the immunosuppressive TME. a Western blot analysis of VEGFR2 and p-VEGFR2 (Tyr1175) expression and qRT-PCR analysis of VEGFR2 expression in A549 and H1299 cells after aptinib treatment for 48 h. The data are presented as mean ± SD (n = 3). **p 

Fig. 3| Enzymatic metabolism of extracellular dT regulated by thymidine phosphorylase (TP)affects tumor functions related to vasculogenic mimicry (VM) formation in hepatocellular carcinoma cells..e Western blot analysis of the expression levels of VE–Cad, vascular endothelial growth factor receptor 1 (VEGFR1), and VEGFR2 influenced by overexpressing TP and adding dT in glucose-free cultured PLC-PRF-5 cells. The ratio of densitometry value to the corresponding glyceraldehyde 3-phosphate dehydrogenase (GAPDH) value was used to indicate the relative protein expression. NG means “No Glucose” (mean ± SD; n = 3 in triplicate; **P < 0.01)

Fig. 5 |Twist1 relies on thymidine phosphorylase (TP) to promote hepatocellular carcinoma (HCC) malignant progression. HCC samples were divided into four groups of Twist1/TP (−/−), Twist1/TP (−/+), Twist1/TP (+/−), and Twist1/TP (+/+) according to the Twist1/TP expression levels. D Analysis of the HCC specimens by IHC. VE–Cad, vascular endothelial growth factor receptor 1 (VEGFR1), and VEGFR2 were minimally expressed in the Twist1- and TP-negative expression groups. When Twist1 and TP were individually or both positively expressed, the expression levels of the three marker proteins increased.

Fig. 7 Effects of hAD-MSCs on ovarian injuries induced by chemotherapy in POI rats after blocking the SDF-1/CXCR4 axis. a Changes in the ovarian tissue were analysed using HE staining (×100 and ×200). b The number of follicles at different stages was counted and compared in the control, POI, hAD-MSCs and hAD-MSCs + AMD3100 groups (n = 10). c Ovarian granulosa cell (GC) apoptosis was tested using the TUNEL assay (×100 and ×400). d The expression levels of Bax, Bcl-2, cleaved-caspase-3, VEGF and VEGFR2 in the ovaries were detected using immunohistochemical staining (×100 and ×400). e–i Semiquantitative analyses of Bax, Bcl-2, cleaved-caspase-3, VEGF and VEGFR2 levels in the ovaries from each group are shown (n = 10). Each dot in the graphs (e–i) represents the value obtained from ten high-power fields (HPFs) randomly chosen from five sections in each group. The bars and error bars in graphs (e–i) indicate the medians and ranges, respectively. Brown cells represent immunostained cells. Representative images are shown. *P < 0.05 and **P < 0.01. Scale bars = 100 μm

Fig. 5| Hsp90β promotes Twist1 nuclear translocation and binding to VE-cadherin promoter to increase VM-related gene networks. eWestern blot analysis of VM and EMT-related markers, including VE-cadherin, VEGFR1, VEGFR2, E-cadherin, Vimentin, MMP2, and MMP9 in PLC-PRF-5 cells overexpressed Hsp90β or under lack of Twist1.