VE-Cadherin Antibody - #AF6265

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产品: VE-Cadherin 抗体
货号: AF6265
描述: Rabbit polyclonal antibody to VE-Cadherin
应用: WB IHC IF/ICC
文献验证: WB, IHC, IF/ICC
反应: Human, Mouse
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Chicken
蛋白号: P33151
RRID: AB_2835123

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 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse
克隆:
Polyclonal
特异性:
VE-Cadherin Antibody detects endogenous levels of total VE-Cadherin.
RRID:
AB_2835123
引用格式: Affinity Biosciences Cat# AF6265, RRID:AB_2835123.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

7B 4; 7B4; 7B4 antigen; CADH5_HUMAN; Cadherin 5; Cadherin 5 type 2; Cadherin 5, type 2 (vascular endothelium); Cadherin 5, type 2, VE cadherin (vascular epithelium); cadherin, vascular endothelial; cadherin, vascular endothelial, 1; Cadherin-5; Cadherin5; CD 144; CD144; CD144 antigen; CDH 5; CDH5; CDH5 protein; Endothelial specific cadherin; FLJ17376; OTTHUMP00000174777; Vascular endothelial cadherin; Vascular epithelium cadherin; VE Cad; VE-cadherin; VEC;

抗原和靶标

免疫原:

A synthesized peptide derived from human VE-Cadherin, corresponding to a region within C-terminal amino acids.

基因/基因ID:
CDH5(1003)
描述:
This gene is a classical cadherin from the cadherin superfamily and is located in a six-cadherin cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer.

研究领域

· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs).   (View pathway)

· Organismal Systems > Immune system > Leukocyte transendothelial migration.   (View pathway)

文献引用

1). 4-Octyl Itaconate Alleviates Myocardial Ischemia-Reperfusion Injury Through Promoting Angiogenesis via ERK Signaling Activation. Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2025 (PubMed: 39836624) [IF=15.1]
2). Precisely co-delivery of protein and ROS scavenger with platesomes for enhanced endothelial barrier preservation against myocardial ischemia reperfusion injury. Chemical Engineering Journal, 2022 [IF=13.3]
3). ZRANB2/SNHG20/FOXK1 Axis regulates Vasculogenic mimicry formation in glioma. JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH, 2019 (PubMed: 30744670) [IF=11.3]

Application: WB    Species: human    Sample: U87 and U251 cells

Fig. 1| Endogenous expression of ZRANB2 and effect of ZRANB2 on biological behaviors of glioma cells. c Protein levels of MMP1, MMP9 and VE-Cadherin regulated by ZRANB2 in U87 and U251 cells. Representative protein expressions and corresponding IDVs of MMP1, MMP9, VE-Cadherin in U87 and U251 are shown; data are presented as mean ± SD (n = 3, each group). *P < 0.05 vs. ZRANB2(−)-NC group, **P < 0.01 vs. ZRANB2(−)-NC group, #P < 0.05 vs. ZRANB2(+)-NC group,##P < 0.01 vs. ZRANB2(+)-NC group.
4). Extracellular vesicle-packaged GBP2 from macrophages aggravates sepsis-induced acute lung injury by promoting ferroptosis in pulmonary vascular endothelial cells. Redox biology, 2025 (PubMed: 40156957) [IF=10.7]

Application: WB    Species: Mouse    Sample:

Fig. 1. Conditioned medium from LPS-stimulated macrophages induces ferroptosis in pulmonary microvascular endothelial cells. (A) Representative immunoblotting and quantification of barrier-associated proteins (ZO-1, VE-Cadherin, and occludin) in the lungs of mice either untreated or subjected to CLP surgery for 6h, 12h, and 24h (n = 3). (B) Representative images and quantification of Evans blue staining in mouse lungs (n = 8). (C) Representative immunofluorescence images of DAPI/CD31/F4/80 staining in mouse lungs. Scale bar = 500 μm. (D) Schematic diagram illustrating the co-culture of septic macrophage-conditioned medium (CM) with human pulmonary microvascular endothelial cells (HPMECs). (E) Representative immunoblotting and quantification of barrier-associated proteins in HPMECs co-cultured with CM, with or without GW4869 treatment (n = 3). (F) Representative images and quantification of PI staining in HPMECs (n = 3). Scale bar = 200 μm. (G, H) Representative images and quantification of liperfluo staining in HPMECs (n = 3). Scale bar = 50 μm. (I, J) C11-BODIPY assay evaluating the lipid ROS levels in HPMECs (n = 3). (K) Representative images for mitochondrial membrane potential of HPMECs (n = 3). Scale bar = 50 μm. (L) Representative immunoblotting and quantification of SLC7A11 and GPX4 in HPMECs (n = 3). Data are presented as mean ± SD.
5). Curcumin-loaded milk-derived sEVs fused with platelet membrane attenuate endothelial senescence and promote spinal cord injury recovery in diabetic mice. Materials today. Bio, 2025 (PubMed: 40688662) [IF=8.7]
6). Oscillating Magnetic Field Regulates Cell Adherence and Endothelialization Based on Magnetic Nanoparticle-Modified Bacterial Cellulose. ACS Applied Materials & Interfaces, 2020 (PubMed: 33170636) [IF=8.3]
7). Qingqi Liangying formula inhibits brain endothelial cell pyroptosis via NLRP3/caspase-1/GSDMD in sepsis-associated encephalopathy. PHYTOMEDICINE, 2025 [IF=8.3]

Application: WB    Species: Mouse    Sample:

Fig. 6. The QL formula preserves BBB integrity in the LPS-induced sepsis model. (A) Schematic of the experimental design, including animal groups, treatment regimens, and timing of EB dye injection. (B) Images depicting EB leakage in brain samples from various groups (n = 5). (C) Quantitative analysis of EB leakage (n = 5). (D) Protein levels of VE-cadherin, MMP-9, occludin, and CAV1 were determined by Western blot, with β-Actin used as a loading control (n = 5). (E–H) Western blot bands for VE-cadherin, MMP-9, occludin, and CAV1 were analyzed semi-quantitatively using ImageJ software. The relative levels of these proteins were calculated based on gray values and normalized to β-Actin (n = 5). (I) CAV1 expression in brain tissues was detected by immunohistochemistry staining (n = 5). Scale bar: 50 μm (upper image), 20 μm (lower image). (J) Collagen IV expression in brain tissues was detected by immunofluorescence staining (n = 3). Scale bar: 100 μm. Data are expressed as mean ± SD.
8). Thymidine phosphorylase promotes malignant progression in hepatocellular carcinoma through pentose Warburg effect. Cell Death & Disease, 2019 (PubMed: 30674871) [IF=8.1]

Application: WB    Species: human    Sample: PLC-PRF-5 cells

Fig. 3| Enzymatic metabolism of extracellular dT regulated by thymidine phosphorylase (TP)affects tumor functions related to vasculogenic mimicry (VM) formation in hepatocellular carcinoma cells..e Western blot analysis of the expression levels of VE–Cad, vascular endothelial growth factor receptor 1 (VEGFR1), and VEGFR2 influenced by overexpressing TP and adding dT in glucose-free cultured PLC-PRF-5 cells. The ratio of densitometry value to the corresponding glyceraldehyde 3-phosphate dehydrogenase (GAPDH) value was used to indicate the relative protein expression. NG means “No Glucose” (mean ± SD; n = 3 in triplicate; **P < 0.01)

Application: IHC    Species: human    Sample: HCC

Fig. 6| Effects of the transcriptional pattern of Twist1 to thymidine phosphorylase (TP) on hepatocellular carcinoma (HCC) growth,metastasis, and vasculogenic mimicry (VM) formation in the xenograft model. d Analysis of Twist1, TP, VE–Cad, vascular endothelial growth factor receptor1 (VEGFR1), and VEGFR2 expression levels in xenograft tumors. Images were taken at 400 magnification.
9). PCDH17 induces colorectal cancer metastasis by destroying the vascular endothelial barrier. Cell death & disease, 2025 (PubMed: 39837826) [IF=8.1]
10). Foxq1 promotes metastasis of nasopharyngeal carcinoma by inducing vasculogenic mimicry via the EGFR signaling pathway. Cell Death & Disease, 2021 (PubMed: 33875643) [IF=8.1]

Application: IF/ICC    Species: mouse    Sample: 5–8F cells

Fig. 7| MiR-124 inhibits the EGFR signaling pathway and VM formation, that could be rescued by Foxq1 expression. E Immunofluorescence staining of Foxq1, EGFR, VE-cadherin, MMP2 and MMP9 in 5–8F cells that overexpressed miR-124, control or simultaneously Foxq1 and miR-124, respectively; scale bars represent 50μm. qRT-PCR (F) and western blot (G) were used to monitor the expression of EGFR signaling pathway and VM-related genes in 5–8F cells that overexpressed miR-124, control or simultaneously Foxq1 and miR-124, respectively.Data are presented as mean ± SD of three independent experiments.

Application: WB    Species: mouse    Sample: 5–8F cells

Fig. 6 |Erlotinib and Nimotuzumab could inhibit Foxq1-induced VM formation and NPC growth and metastasis in vivo.F Statistical results of metastatic nodules of each group; p < 0.001. The expression of related genes in xenograft tumors from each group were monitored by qRT-PCR (G) and western blot (H).
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