CDC25A Antibody - #AF6252

Fig. 6. Knockdown of SGLT1 suppresses inflammatory response and pyroptosis in GVDM mice. A, Protein levels of NLRP3, ASC and caspase-1 (p20) in left ventricle tissues were measured by western blot. B, Quantitative analysis of relative protein levels of NLRP3, ASC and caspase-1. C, Protein levels of full length GSDMD and cleaved GSDMD were measured by western blot. D, Quantitative analysis of relative protein levels of cleaved GSDMD. E, The expression of NLRP3 was detected by immunofluorescence. Scale bar: 50 μm. F, Caspase-1 activity was measured by commercial kit. The relative mRNA levels of IL-1β (G) and IL-18 (H) were detected by qRT-PCR. I, The protein levels of IL-1β, pro-IL-1β and IL-18 were measured by western blot. Quantitative analysis of relative protein levels of IL-1β (J) and IL-18 (K). The release of IL-1β (L) and IL-18 (M) were detected by ELISA. Data were expressed as mean ± Standard deviation (SD). ***p < 0.001, **p < 0.01, *p < 0.05 vs control mice; †††p < 0.001, ††p < 0.01, † p < 0.05 vs DM mice; ###p < 0.001, ##p < 0.01 vs GVDM+shNC mice. DM, diabetes mellitus; GV, glycemic variability; SGLT1, sodium-glucose cotransporter 1; shSGLT1, SGLT1 shRNA; shNC, negative control shRNA; NLRP3, NLR family pyrin domain containing 3; ASC, apoptosis-associated speck-like protein containing CARD domain; GSDMD, gasdermin D.

Figure 3. |M22 regulates G0/G1 arrest through a negative feedback mechanism. (A) M22 attenuates mRNA expression of Cyclin D1, Cyclin E1, CDK4, CDK6, CDC25A and PCNA genes in A549 cells for 24 h. RNA was isolated and analsis was performed to detect by qRT-PCR. *Means P < 0.01, ***means P < 0.0001. (B) M22 down-regulates the expression of Cyclin D1 and CDC25C protein in A549 cells by Western blotting.

Figure 5. |SMYD3 regulated ovarian cancer proliferation and apoptosis though SMYD3-H4K20me3-CDKN2A and SMYD3-H3K4me3-BIRC3, respectively. (A-B) The protein expression changes in the above 6 genes in the HEY and A2780 cell lines after SMYD3 knockdown in HEY and A2780 cells.

Figure 5: |The effect of DDTC on expression of cell cycle regulators. The dosage of DDTC at 0.1 μM, 1 μM, and 10 μM in SKOV3 and A2780 cells. (a, b) Western blot assay showed that DDTC upregulated the expression of Chk1 protein level and downregulated the expression of Cdc25a and CDK1protein levels in SKOV3 cells.