产品: SENP1 抗体
货号: AF0275
描述: Rabbit polyclonal antibody to SENP1
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 73kDa; 73kD(Calculated).
蛋白号: Q9P0U3
RRID: AB_2833445

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(92%), Sheep(100%), Rabbit(91%), Dog(100%)
克隆:
Polyclonal
特异性:
SENP1 Antibody detects endogenous levels of total SENP1.
RRID:
AB_2833445
引用格式: Affinity Biosciences Cat# AF0275, RRID:AB_2833445.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

SENP 1; SENP1; SENP1_HUMAN; Sentrin specific protease 1; Sentrin-specific protease 1; Sentrin/SUMO specific protease 1; Sentrin/SUMO specific protease; Sentrin/SUMO specific protease SENP 1; Sentrin/SUMO specific protease SENP1; Sentrin/SUMO-specific protease SENP1; SUMO1/sentrin specific peptidase 1; SUMO1/sentrin specific protease 1; SuPr 2; SuPr2;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
Q9P0U3 SENP1_HUMAN:

Highly expressed in testis. Expressed at lower levels in thymus, pancreas, spleen, liver, ovary and small intestine.

描述:
SENP1 Protease that catalyzes two essential functions in the SUMO pathway: processing of full-length SUMO1, SUMO2 and SUMO3 to their mature forms and deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins. Deconjugates SUMO1 from HIPK2. Deconjugates SUMO1 from HDAC1, which decreases its transcriptional repression activity.
序列:
MDDIADRMRMDAGEVTLVNHNSVFKTHLLPQTGFPEDQLSLSDQQILSSRQGHLDRSFTCSTRSAAYNPSYYSDNPSSDSFLGSGDLRTFGQSANGQWRNSTPSSSSSLQKSRNSRSLYLETRKTSSGLSNSFAGKSNHHCHVSAYEKSFPIKPVPSPSWSGSCRRSLLSPKKTQRRHVSTAEETVQEEEREIYRQLLQMVTGKQFTIAKPTTHFPLHLSRCLSSSKNTLKDSLFKNGNSCASQIIGSDTSSSGSASILTNQEQLSHSVYSLSSYTPDVAFGSKDSGTLHHPHHHHSVPHQPDNLAASNTQSEGSDSVILLKVKDSQTPTPSSTFFQAELWIKELTSVYDSRARERLRQIEEQKALALQLQNQRLQEREHSVHDSVELHLRVPLEKEIPVTVVQETQKKGHKLTDSEDEFPEITEEMEKEIKNVFRNGNQDEVLSEAFRLTITRKDIQTLNHLNWLNDEIINFYMNMLMERSKEKGLPSVHAFNTFFFTKLKTAGYQAVKRWTKKVDVFSVDILLVPIHLGVHWCLAVVDFRKKNITYYDSMGGINNEACRILLQYLKQESIDKKRKEFDTNGWQLFSKKSQEIPQQMNGSDCGMFACKYADCITKDRPINFTQQHMPYFRKRMVWEILHRKLL

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Horse
92
Rabbit
91
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q9P0U3 作为底物

Site PTM Type Enzyme
S57 Phosphorylation
T59 Phosphorylation
S78 Phosphorylation
S80 Phosphorylation
T102 Phosphorylation
K111 Ubiquitination
S117 Phosphorylation
K124 Methylation
K124 Ubiquitination
S126 Phosphorylation
S127 Phosphorylation
S130 Phosphorylation
S132 Phosphorylation
K136 Methylation
K136 Ubiquitination
S149 Phosphorylation
K153 Ubiquitination
S157 Phosphorylation
S159 Phosphorylation
S161 Phosphorylation
S167 Phosphorylation
S170 Phosphorylation
S180 Phosphorylation
K210 Ubiquitination
S224 Phosphorylation
S226 Phosphorylation
K227 Ubiquitination
K231 Ubiquitination
S233 Phosphorylation
T328 Phosphorylation
T330 Phosphorylation
Y349 Phosphorylation
K364 Acetylation
K364 Ubiquitination
S381 Phosphorylation
K396 Acetylation
K396 Ubiquitination
T414 Phosphorylation
S416 Phosphorylation
K429 Acetylation
K429 Ubiquitination
K432 Ubiquitination
K502 Ubiquitination
K510 Ubiquitination
K577 Ubiquitination
K609 Ubiquitination
K616 Ubiquitination

研究背景

功能:

Protease that catalyzes two essential functions in the SUMO pathway. The first is the hydrolysis of an alpha-linked peptide bond at the C-terminal end of the small ubiquitin-like modifier (SUMO) propeptides, SUMO1, SUMO2 and SUMO3 leading to the mature form of the proteins. The second is the deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins, by cleaving an epsilon-linked peptide bond between the C-terminal glycine of the mature SUMO and the lysine epsilon-amino group of the target protein. Deconjugates SUMO1 from HIPK2. Deconjugates SUMO1 from HDAC1 and BHLHE40/DEC1, which decreases its transcriptional repression activity. Deconjugates SUMO1 from CLOCK, which decreases its transcriptional activation activity. Deconjugates SUMO2 from MTA1. Deconjugates SUMO1 from METTL3. Desumoylates CCAR2 which decreases its interaction with SIRT1. Deconjugates SUMO1 from GPS2.

细胞定位:

Nucleus. Cytoplasm.
Note: Shuttles between cytoplasm and nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Highly expressed in testis. Expressed at lower levels in thymus, pancreas, spleen, liver, ovary and small intestine.

亚基结构:

Interacts with MTA1. Interacts with CCAR2 (via N-terminus).

蛋白家族:

Belongs to the peptidase C48 family.

文献引用

1). Hypoxia maintains the fenestration of liver sinusoidal endothelial cells and promotes their proliferation through the SENP1/HIF-1α/VEGF signaling axis. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (PubMed: 33445109) [IF=3.1]

Application: WB    Species: mouse    Sample: Liver sinusoidal endothelial cells (LSECs)

Fig. 2. After hypoxic culture of mouse LSECs, the expressions of SENP1,HIF-1a,and VEGF are all up-regulated.(A)(B)(C)real-time PCR showing SENP1 or HIF-1a or VEGF mRNA after a certain period of hypoxia culture.The date are presented as mean ± SEM.**p < 0.01,***p < 0.001,****p < 0.0001,n ¼ 3per group.(D)Western results showing the expression level of SENP1 and HIF-1a after a certain time of hypoxia culture.(E)(F) ratio of SENP1 or HIF-1a vs b-actin in western results.The date are presented as mean ± SEM.*p < 0.05,**p < 0.01,***p < 0.001,n ¼ 3 per group.(G) ELISA assay of VEGF production in LSECs after a certain time of hypoxia culture.The date are presented as mean ± SEM.****p < 0.0001,n ¼ 3 per group.

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