SHP1 Antibody - #AF6244

Fig. 3. AgB diminishes the phagocytic capacity of M1 macrophages. (A). THP-1 cells were stimulated to differentiate into M1 macrophages and then challenged with phagocytosis of IgG-coated latex beads. Scale bar, 50 μm. (B). The phagocytic index of M1 macrophages, calculated as the ratio of IgG-coated latex bead-positive macrophages at 37 °C versus 4 °C, was determined using fluorescence-activated cell sorting (FACS) analysis. (C–F). Quantitative assessment of the mRNA expression levels of FcγRI, FcγRIIa, FcγRIIb, and FcγRIII in M1 macrophages. (G) Mouse peritoneal macrophages were engaged in the phagocytosis of opsonized platelets, and the phagocytic index was calculated by multiplying the average fluorescence intensity by the frequency of DiI-positive cells. (H) The uptake of opsonized zymosan A particles by bone marrow-derived macrophages (BMDMs) from mice was assessed. (I) The phagocytosis of opsonized sheep red blood cells (SRBCs) by mouse BMDMs was evaluated. (J) Western blot analysis was conducted to examine the impact of AgB on the phosphorylation of ERK1/2, AKT, p38, and SHP-1 in mouse BMDMs under various conditions. Right panel: Quantitative analysis of ERK1/2, AKT, p38, and SHP-1 phosphorylation levels. Data represent the mean ± SEMs from two to three independent experiments (n = 6). ∗, P < 0.05; ∗∗, P < 0.01; ##, P < 0.01. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Figure 8 SPN enhanced TGR5 and FXR expression in the D-gal-induced mice. (A) IHC analysis of TGR5 and FXR in femurs (black arrows, n=5). (B) Western blotting analysis of TGR5, FXR, and SHP in tibias (n=3). (C) TGR5, FXR and SHP mRNA in tibias (n=5). Data are expressed as the mean ± SD. **P < 0.01 compared with the MOD group.