CDK2 Antibody - #AF6237

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产品: CDK2 抗体
货号: AF6237
描述: Rabbit polyclonal antibody to CDK2
应用: WB IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
蛋白号: P24941
RRID: AB_2835103

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
CDK2 Antibody detects endogenous levels of total CDK2.
RRID:
AB_2835103
引用格式: Affinity Biosciences Cat# AF6237, RRID:AB_2835103.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Cdc2 related protein kinase; cdc2-related protein kinase; CDC28; CDC2A; Cdk 2; CDK1; CDK2; CDK2_HUMAN; CDKN2; Cell devision kinase 2; Cell division protein kinase 2; Cyclin dependent kinase 2; cyclin dependent kinase 2-alpha; Cyclin-dependent kinase 2; kinase Cdc2; MPF; p33 protein kinase; p33(CDK2);

抗原和靶标

免疫原:

A synthesized peptide derived from human CDK2, corresponding to a region within the internal amino acids.

基因/基因ID:
CDK2(1017)
描述:
The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle.

研究领域

· Cellular Processes > Cell growth and death > Cell cycle.   (View pathway)

· Cellular Processes > Cell growth and death > Oocyte meiosis.   (View pathway)

· Cellular Processes > Cell growth and death > p53 signaling pathway.   (View pathway)

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

· Environmental Information Processing > Signal transduction > FoxO signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > Measles.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

· Human Diseases > Cancers: Specific types > Prostate cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Small cell lung cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Gastric cancer.   (View pathway)

· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.

文献引用

1). Sesamol serves as a p53 stabilizer to relieve rheumatoid arthritis progression and inhibits the growth of synovial organoids. Phytomedicine : international journal of phytotherapy and phytopharmacology, 2023 (PubMed: 37778247) [IF=6.7]
2). circ0066187 promotes pulmonary fibrogenesis through targeting STAT3-mediated metabolism signal pathway. Cellular and molecular life sciences : CMLS, 2025 (PubMed: 39969586) [IF=6.2]
3). Silica nanoparticles cause spermatogenesis dysfunction in mice via inducing cell cycle arrest and apoptosis. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, 2022 (PubMed: 35051769) [IF=6.2]

Application: WB    Species: mouse    Sample: testis

Fig. 5. |SiNPs affected the protein expressions of cell cycle regulators. (A) The protein expressions of cyclin A1, CDK2, cyclin E1, CDK1, and cyclin B1 were significantly decreased in the testis after exposure to SiNPs.
4). Isobavachalcone, a natural sirtuin 2 inhibitor, exhibits anti-triple-negative breast cancer efficacy in vitro and in vivo. Phytotherapy research : PTR, 2024 (PubMed: 38349045) [IF=6.1]
5). Leptin promotes angiogenesis via pericyte STAT3 pathway upon intracerebral hemorrhage. Cells, 2022 (PubMed: 36078162) [IF=6.0]
6). Knockdown of DNMT1 Induces SLCO3A1 to Promote Follicular Growth by Enhancing the Proliferation of Granulosa Cells in Mammals. International journal of molecular sciences, 2024 (PubMed: 38473715) [IF=5.6]

Application: WB    Species: Mouse    Sample: GCs

Figure 2. SLCO3A1 promotes the cell cycle-related processes but inhibits the apoptosis of GCs. The cell cycle distribution was analyzed by flow cytometric transfected with OE-SLCO3A1 (A) or KD-SLCO3A1 (B). Green: cells in the G0-G1 phase, Yellow: cells in S phase, Blue: cells in G2-M phase. (C) RT-qPCR analysis of cycle-related genes expression transfected with OE-NC or OE-SLCO3A1. (D) Western blot analysis of CCNE1, CDK2 and CCND1 transfected with OE-NC or OE-SLCO3A1. (E) RT-qPCR analysis of cycle-related genes expression transfected with KD-NC or KD-SLCO3A1. (F) Western blot analysis of CCNE1, CDK2 and CCND1 transfected with KD-NC or KD-SLCO3A1. Flow cytometry assay of the apoptosis rate transfected with OE-SLCO3A1 (G) or KD-SLCO3A1 (H). Q1: dead cells and cell debris, Q2: late apoptotic cells, Q3: early apoptotic cells, Q4: negative cells. RT-qPCR analysis of pro-apoptosis related genes expression transfected with OE-SLCO3A1 (I) or KD-SLCO3A1 (K). Western blot analysis of CASP3 and CASP8 transfected with OE-SLCO3A1 (J) or KD-SLCO3A1 (L). All of the above experimental results were obtained in the COV434 cells. * p < 0.05, ** p < 0.01, *** p < 0.001, ns p > 0.05.
7). Immortalization-upregulated protein promotes pancreatic cancer progression by regulating NPM1/FHL1-mediated cell-cycle-checkpoint protein activity. Cell Biology and Toxicology, 2022 (PubMed: 35142956) [IF=5.3]
8). G-4 inhibits triple negative breast cancer by inducing cell apoptosis and promoting LCN2-dependent ferroptosis. Biochemical pharmacology, 2024 (PubMed: 38395264) [IF=5.3]
9). Activation of ATM/Chk2 by Zanthoxylum armatum DC extract induces DNA damage and G1/S phase arrest in BRL 3A cells. JOURNAL OF ETHNOPHARMACOLOGY, 2022 (PubMed: 34775036) [IF=4.8]
10). The Synergistic Mechanism of Chelidonium majus Alkaloids on Melanoma Treatment via a Multi-Strategy Insight. Molecules (Basel, Switzerland), 2024 (PubMed: 39598801) [IF=4.6]
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