PKR Antibody - #AF6216

Fig. 6 CX3CL1 deficiency inhibited cisplatin-induced ER stress and HIF1A/HO-1 signal activation in kidney tissues. A Western blot analysis of proteins in kidney tissues that are associated with ER stress. B Western blotting was conducted to evaluate the HIF1A and HO-1 protein expression patterns in renal tissues. C Representative micrographs of CHOP and nephrin staining in renal tissues. Scale bar = 10 μm. D Representative micrographs of HO-1 and nephrin staining in renal tissues. Scale bar = 10 μm. E Western blot analysis was conducted to analyze the podocyte expression profiles of proteins associated with ER stress. F Podocytes’ HIF1A and HO-1 expression profiles were evaluated by western blotting. G Representative micrographs of podocytes stained with eIF2α and p-eIF2α. Scale bar = 10 μm. H Representative micrographs of podocytes stained with HIF1A and HO-1. Scale bar = 10 μm. (ER, endoplasmic reticulum; HIF1A/HO-1, hypoxia inducible factor 1-alpha/heme oxygenase-1; CHOP, CCAAT enhancer binding protein-homologous protein; eIF2α, eukaryotic translation initiation factor 2A; P value was calculated by one-way analysis of variance and Tukey’s test.

Fig. 6 ITGA11 inhibits prostate cancer cell migration and invasion through PI3K/AKT signaling. a Overlapping coexpressed genes of ITGA11 in five cohorts and KEGG pathway enrichment analysis. b Correlation between ITGA11 expression and the activation level of PI3K/AKT signaling assessed by the HALLMARK PI3K/AKT/mTOR signaling signature. c Correlation between ITGA11 expression and the activation level of PI3K/AKT signaling assessed by the REACTOME PI3K/AKT activation signature. d Western blot analysis validated the inhibition of Ser473 p-AKT1/2/3 activation via knockdown of shITGA11 in both C4-2 and PC-3 cells. e Quantification of the western blotting results showing in d. Data are presented as the mean ± SD based on three independent experiments (ns not significant, *P < 0.05, **P < 0.01 by one-way ANOVA), bars represent mean values, error bars represent SD.