产品: Epo-R 抗体
货号: AF6211
描述: Rabbit polyclonal antibody to Epo-R
应用: WB IF/ICC
反应: Human, Mouse, Rat, Monkey
预测: Pig, Sheep, Dog
分子量: 65kDa; 55kD(Calculated).
蛋白号: P19235
RRID: AB_2835092

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat,Monkey
预测:
Pig(86%), Sheep(86%), Dog(86%)
克隆:
Polyclonal
特异性:
Epo-R Antibody detects endogenous levels of total Epo-R.
RRID:
AB_2835092
引用格式: Affinity Biosciences Cat# AF6211, RRID:AB_2835092.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

EPO R; EPO-R; epor; EPOR_HUMAN; Erythropoietin receptor; Erythropoietin receptor precursor; MGC138358;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
P19235 EPOR_HUMAN:

Erythroid cells and erythroid progenitor cells. Isoform EPOR-F is the most abundant form in EPO-dependent erythroleukemia cells and in late-stage erythroid progenitors. Isoform EPOR-S and isoform EPOR-T are the predominant forms in bone marrow. Isoform EPOR-T is the most abundant from in early-stage erythroid progenitor cells.

描述:
Erythropoiesis is regulated through the interaction of erythropoietin (Epo) with its receptor, EpoR, a member of the cytokine superfamily of receptors. The human EpoR is a 507 amino acid transmembrane protein that forms homodimers following erythropoietin activation and is related to the interleukin 2 (IL-2) receptor β chain subunit (IL-2Rβ).
序列:
MDHLGASLWPQVGSLCLLLAGAAWAPPPNLPDPKFESKAALLAARGPEELLCFTERLEDLVCFWEEAASAGVGPGNYSFSYQLEDEPWKLCRLHQAPTARGAVRFWCSLPTADTSSFVPLELRVTAASGAPRYHRVIHINEVVLLDAPVGLVARLADESGHVVLRWLPPPETPMTSHIRYEVDVSAGNGAGSVQRVEILEGRTECVLSNLRGRTRYTFAVRARMAEPSFGGFWSAWSEPVSLLTPSDLDPLILTLSLILVVILVLLTVLALLSHRRALKQKIWPGIPSPESEFEGLFTTHKGNFQLWLYQNDGCLWWSPCTPFTEDPPASLEVLSERCWGTMQAVEPGTDDEGPLLEPVGSEHAQDTYLVLDKWLLPRNPPSEDLPGPGGSVDIVAMDEGSEASSCSSALASKPSPEGASAASFEYTILDPSSQLLRPWTLCPELPPTPPHLKYLYLVVSDSGISTDYSSGDSQGAQGGLSDGPYSNPYENSLIPAAEPLPPSYVACS

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
86
Sheep
86
Dog
86
Horse
71
Bovine
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P19235 作为底物

Site PTM Type Enzyme
S108 Phosphorylation
S115 Phosphorylation
S116 Phosphorylation
S185 Phosphorylation
S192 Phosphorylation
Y309 Phosphorylation
Y368 Phosphorylation O60674 (JAK2) , P07948 (LYN)
Y426 Phosphorylation
Y454 Phosphorylation
Y456 Phosphorylation
Y468 Phosphorylation
Y485 Phosphorylation
Y489 Phosphorylation
Y504 Phosphorylation

研究背景

功能:

Receptor for erythropoietin. Mediates erythropoietin-induced erythroblast proliferation and differentiation. Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade. In some cell types, can also activate STAT1 and STAT3. May also activate the LYN tyrosine kinase.

Isoform EPOR-T acts as a dominant-negative receptor of EPOR-mediated signaling.

翻译修饰:

On EPO stimulation, phosphorylated on C-terminal tyrosine residues by JAK2. The phosphotyrosine motifs are also recruitment sites for several SH2-containing proteins and adapter proteins which mediate cell proliferation. Phosphorylation on Tyr-454 is required for PTPN6 interaction, Tyr-426 for PTPN11. Tyr-426 is also required for SOCS3 binding, but Tyr-454/Tyr-456 motif is the preferred binding site.

Ubiquitination at Lys-281 mediates receptor internalization, whereas ubiquitination at Lys-453 promotes trafficking of activated receptors to the lysosomes for degradation (By similarity). Ubiquitinated by NOSIP; appears to be either multi-monoubiquitinated or polyubiquitinated. Ubiquitination mediates proliferation and survival of EPO-dependent cells.

细胞定位:

Cell membrane>Single-pass type I membrane protein.

Secreted.
Note: Secreted and located to the cell surface.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Erythroid cells and erythroid progenitor cells. Isoform EPOR-F is the most abundant form in EPO-dependent erythroleukemia cells and in late-stage erythroid progenitors. Isoform EPOR-S and isoform EPOR-T are the predominant forms in bone marrow. Isoform EPOR-T is the most abundant from in early-stage erythroid progenitor cells.

亚基结构:

Forms homodimers on EPO stimulation. The tyrosine-phosphorylated form interacts with several SH2 domain-containing proteins including LYN (By similarity), the adapter protein APS, PTPN6 (By similarity), PTPN11, JAK2, PI3 kinases, STAT5A/B, SOCS3, CRKL (By similarity). Interacts with INPP5D/SHIP1 (By similarity). The N-terminal SH2 domain of PTPN6 binds Tyr-454 and inhibits signaling through dephosphorylation of JAK2 (By similarity). APS binding also inhibits the JAK-STAT signaling. Binding to PTPN11, preferentially through the N-terminal SH2 domain, promotes mitogenesis and phosphorylation of PTPN11 (By similarity). Binding of JAK2 (through its N-terminal) promotes cell-surface expression (By similarity). Interacts with RHEX; this interaction occurs in a erythropoietin (EPO)-dependent manner. Interaction with the ubiquitin ligase NOSIP mediates EPO-induced cell proliferation. Interacts with ATXN2L.

蛋白家族:

The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.

The box 1 motif is required for JAK interaction and/or activation.

Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.

Belongs to the type I cytokine receptor family. Type 1 subfamily.

研究领域

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Organismal Systems > Immune system > Hematopoietic cell lineage.   (View pathway)

文献引用

1). Chen et al. The Effects of Curcumae Longae Radix, Curcuma phaeocaulis Radix and Their Processed Products on Epo/EpoR Pathway and CD62p. Frontiers in Pharmacology 2018 Jul 9;9:736 (PubMed: 30038572) [IF=5.6]

Application: WB    Species: rat    Sample: hepar

FIGURE 4 | Observation for EpoR protein expression in hepar. CG, control group, MG, model group, SHG, Curcumae Longae Radix group, CHG,Stir-frying Curcumae Longae Radix with vinegar group, JHG, Stir-frying Curcumae Longae Radix with wine group, SLG, Curcuma phaeocaulis Radix group, CLG, Stir-frying Curcuma phaeocaulis Radix with vinegar group, JLG,Stir-frying Curcuma phaeocaulis Radix with wine group and PoG, positive group. Values were mean ± SD (n = 6). ∗p < 0.05 and ∗∗p < 0.01 vs. Control group #p < 0.05 and ##p < 0.01 vs. model group 1p < 0.05 and P < 0.01 Curcuma phaeocaulis Radix group versus Curcumae Longae Radix group, Stir-frying Curcuma phaeocaulis Radix with vinegar group versus Stir-frying Curcumae Longae Radix with vinegar group, Stir-frying Curcuma phaeocaulis Radix with wine group versus Stir-frying Curcumae Longae Radix with wine group.

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