ATOX1 Antibody - #DF13934

Fig. 1 Effects of curcumin on ATOX1-associated copper chaperones in A549 and H1299 cells. (A) The binding activity between curcumin and ATOX1 protein was analyzed using molecular docking. (B) Cell viability was measured by CCK-8. (C) Levels of ATOX1, ATP7A, and COX17 proteins were measured by western blotting. The levels of proteins were normalized by β-actin. The full-length blots were shown in Supplementary Fig. 1 and Fig. 2. (D) The semi-quantitative analysis of the blots was analyzed by ImageJ software. (E) Effects of DC-AC50 treatment on cell viability. Three parallel experiments were performed in cells. NS no significance. *p 

Fig. 5 Curcumin inhibited tumor growth by suppressing ATOX1. The animal model was established by injection of A549 cells. After 6 days of injection, the mice were injected with 20 mg/kg/d DC-AC50 or 40 mg/kg/d curcumin by intraperitoneal injection for 21 days. (A) Tumor volume was measured every week. (B) Tumor weight was obtained. Expressions of ATOX1 in tumor tissues were measured by immunohistochemistry (C) and western blotting (D). Black arrows: ATOX1 expression. The full-length blots were presented in Supplementary Fig. 7. Six animals in each group. *p