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产品描述
来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
反应:
Human, Rat
克隆:
Polyclonal
特异性:
RSPH4A Antibody detects endogenous levels of total RSPH4A.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:
展开/折叠
A230081C05; CILD11; dJ412I7.1; Radial spoke head protein 4 homolog A; Radial spoke head-like protein 3; RSH4A_HUMAN; RSHL3; Rsph4a; RSPH6B;
抗原和靶标
文献引用
1). Association of novel DNAH11 variants with asthenoteratozoospermia lead to male infertility. Human genomics, 2024
(PubMed: 39256880)
[IF=3.8]
Application: WB Species: human Sample:
Fig. 5 The distribution and expression levels of flagella-associated proteins in patients. (A-D). Immunofluorescence staining assays were performed on the sperm of mutant patients and normal subjects using anti-DNAI2 (red), anti-SPAG6 (red), anti-RSPH4A (red), and anti-TOMM20 (red). The results showed that TOMM20 was located at the mitochondrial sheath, and RSPH4A, DNAI2, and SPAG6 were distributed in full-length flagella in fertile control individual sperm. By contrast, it was found that almost absence of DNAI2, SPAG6, and TOMM20 in sperm from AY0524 and AY0540, RSPH4A was accumulated on the sperm neck, while it was not evidently changed in DNAH9-deficient sperm. Anti-ac-tubulin (green) marked the sperm flagella, and Hoechst (blue) marked the nucleus of spermatozoa. Scale bars: 10 μm. (E) The levels of DNAI2, SPAG6, RSPH4A, and TOMM20 in sperm of patients (AY0524, AY0540, XFY03, and XFY04) reduced markedly compared with control individuals by western blotting analysis. The results of WB assays were in accordance with those of immunofluorescence assays described above. β-actin was used as an internal reference
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