PKC alpha Antibody - #AF6196

>>买2送1 进行中>>
>>赠JD卡 进行中>>
(6)   (5)  
产品: PKC alpha 抗体
货号: AF6196
描述: Rabbit polyclonal antibody to PKC alpha
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
蛋白号: P17252
RRID: AB_2835077

浏览相似产品>>

   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

联系销售
相关下载
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
PKC alpha Antibody detects endogenous levels of total PKC alpha.
RRID:
AB_2835077
引用格式: Affinity Biosciences Cat# AF6196, RRID:AB_2835077.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

AAG6; Aging associated gene 6; aPKC; KPCA_HUMAN; PKC alpha; PKC-A; PKC-alpha; PKCA; PRKACA; PRKCA; Protein Kinase C alpha; Protein kinase C alpha type;

抗原和靶标

免疫原:

A synthesized peptide derived from human PKC alpha, corresponding to a region within C-terminal amino acids.

基因/基因ID:
PRKCA(5578)
描述:
PKC alpha is an AGC kinase of the PKC family. A classical PKC downstream of many mitogenic and receptors. Classical PKCs are calcium-dependent enzymes that are activated by phosphatidylserine, diacylglycerol and phorbol esters.

研究领域

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Gap junction.   (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > ErbB signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Calcium signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Phosphatidylinositol signaling system.

· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > mTOR signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Wnt signaling pathway.   (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.

· Human Diseases > Substance dependence > Amphetamine addiction.

· Human Diseases > Substance dependence > Morphine addiction.

· Human Diseases > Infectious diseases: Bacterial > Vibrio cholerae infection.

· Human Diseases > Infectious diseases: Bacterial > Pathogenic Escherichia coli infection.

· Human Diseases > Infectious diseases: Parasitic > African trypanosomiasis.

· Human Diseases > Infectious diseases: Parasitic > Amoebiasis.

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Human Diseases > Cancers: Specific types > Glioma.   (View pathway)

· Human Diseases > Cancers: Specific types > Non-small cell lung cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma.   (View pathway)

· Human Diseases > Cancers: Overview > Choline metabolism in cancer.   (View pathway)

· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes.   (View pathway)

· Organismal Systems > Circulatory system > Vascular smooth muscle contraction.   (View pathway)

· Organismal Systems > Development > Axon guidance.   (View pathway)

· Organismal Systems > Immune system > Natural killer cell mediated cytotoxicity.   (View pathway)

· Organismal Systems > Immune system > Fc epsilon RI signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis.   (View pathway)

· Organismal Systems > Immune system > Leukocyte transendothelial migration.   (View pathway)

· Organismal Systems > Environmental adaptation > Circadian entrainment.

· Organismal Systems > Nervous system > Long-term potentiation.

· Organismal Systems > Nervous system > Retrograde endocannabinoid signaling.   (View pathway)

· Organismal Systems > Nervous system > Glutamatergic synapse.

· Organismal Systems > Nervous system > Cholinergic synapse.

· Organismal Systems > Nervous system > Serotonergic synapse.

· Organismal Systems > Nervous system > GABAergic synapse.

· Organismal Systems > Nervous system > Dopaminergic synapse.

· Organismal Systems > Nervous system > Long-term depression.

· Organismal Systems > Sensory system > Inflammatory mediator regulation of TRP channels.   (View pathway)

· Organismal Systems > Endocrine system > Insulin secretion.   (View pathway)

· Organismal Systems > Endocrine system > Melanogenesis.

· Organismal Systems > Endocrine system > Thyroid hormone synthesis.

· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Oxytocin signaling pathway.

· Organismal Systems > Endocrine system > Aldosterone synthesis and secretion.

· Organismal Systems > Endocrine system > Relaxin signaling pathway.

· Organismal Systems > Excretory system > Aldosterone-regulated sodium reabsorption.

· Organismal Systems > Excretory system > Endocrine and other factor-regulated calcium reabsorption.

· Organismal Systems > Digestive system > Salivary secretion.

· Organismal Systems > Digestive system > Gastric acid secretion.

· Organismal Systems > Digestive system > Pancreatic secretion.

文献引用

1). Upregulation of UGT1A1 Expression by Ursolic Acid and Oleanolic Acid via the Inhibition of the PKC/NF-κB Signaling Pathway. Phytomedicine, 2021 (PubMed: 34536821) [IF=6.7]

Application: WB    Species: Human    Sample: HepG2 cells

Fig 4. Effects of UA and OA on PMA-induced PKC activation in HepG2 cells. HepG2 cells were treated with 40 μ M UA or OA (alone), or they were incubated with PMA (100 nM) in the presence or absence of 40 μ M UA or OA for 24 h. The expression levels of relative PKC α protein (total, membrane, and cytoplasm PKC α , as well as phospho-PKC α were quantified by western blotting, normalized to GAPDH, and compared to the vehicle-treated group or PMA-treated group. DMSO (0.1%) was used as negative control. The values were expressed as the mean ± S.E. of three independent experiments. * p < 0.05, ** p < 0.01 relative to vehicle-treated wells; ## p < 0.01 relative to PMA-treated wells.
2). Phosphorylated CPI-17 and MLC2 as Biomarkers of Coronary Artery Spasm-Induced Sudden Cardiac Death. International journal of molecular sciences, 2024 (PubMed: 38474189) [IF=5.6]

Application: WB    Species: Rat    Sample:

Figure 5 PKC was activated during contraction of CAS coronary arteries in vivo (n = 5 per group). Pit-induced PKC translocation in coronary arteries. (A) Representative WB shows PKCα in cytosolic and membrane particulate fractions. Results are expressed as the grayscale ratio of cytoplasmic PKCα to cell membrane PKCα. (B) Representative WB shows PKCδ in cytosolic and membrane particulate fractions. Results are expressed as the grayscale ratio of cytoplasmic PKCδ to cell membrane PKCδ. (C) Representative WB shows PKCε in cytosolic and membrane particulate fractions. Results are expressed as the grayscale ratio of cytoplasmic PKCε to cell membrane PKCε. Data presented as mean ± SEM. ns, no significance; *** p < 0.001 vs. control group. PKC: protein kinase C.
3). All-Trans Retinoic Acid Impairs Platelet Function and Thrombus Formation and Inhibits Protein Kinase CßI/δ Phosphorylation. THROMBOSIS AND HAEMOSTASIS, 2019 (PubMed: 31370073) [IF=5.0]

Application: WB    Species: human    Sample: human platelets

Fig. 6| Phosphorylation of protein kinase C (PKC) α/βII, PKCßI and PKCδ. All-trans retinoic acid (ATRA)-treated human platelets were stimulated with 5 μg/mL collagen-related peptide (CRP) (A) or 1 U/mL thrombin (under the conditions of clot retraction) (B) for 15 minutes and the phosphorylation level of PKCα/βII, PKCßI, and PKCδ was measured by Western blot.
4). Processed product (Pinelliae Rhizoma Praeparatum) of Pinellia ternata (Thunb.) Breit. Alleviates the allergic airway inflammation of cold phlegm via regulation of PKC/EGFR/MAPK/PI3K-AKT signaling pathway. JOURNAL OF ETHNOPHARMACOLOGY, 2022 (PubMed: 35688257) [IF=4.8]
5). Anti-Malignant Ascites Effect of Total Diterpenoids from Euphorbiae ebracteolatae Radix Is Attributable to Alterations of Aquaporins via Inhibiting PKC Activity in the Kidney. MOLECULES, 2021 (PubMed: 33578967) [IF=4.6]

Application: WB    Species: mice    Sample: kidneys

Figure 4. Effects of total diterpenoids from the root of Euphorbia ebracteolata Hayata (TDEE) on protein kinase C (PKC) and protein kinase A (PKA) activation in the kidneys of H22 tumor cell-bearing mice. (A) Western blot band and protein relative expression of PKCα. (B) Western blot band and protein relative expression of PKCβ. (C) Western blot band and protein relative expression of PKA. (D) Western blot bands and protein relative expression of phospholipase C (PLC) and phosphorylated PLC. Normal: normal mice treated with blank solvent. Model: H22 tumor cell-bearing mice treated with blank solvent. The data are represented as the mean ± SD, n = 3, * p < 0.05 vs. model group (Mann–Whitney U test).
6). Sodium butyrate enhances titanium nail osseointegration in ovariectomized rats by inhibiting the PKCα/NOX4/ROS/NF-κB pathways. Journal of orthopaedic surgery and research, 2023 (PubMed: 37528483) [IF=2.8]

限制条款

产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）

产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。 

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.