(5)
(4)
产品描述
来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
MYOCD Antibody detects endogenous levels of total MYOCD.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:
展开/折叠
MYCD; MYCD_HUMAN; Myocardin; Myocd;
抗原和靶标
免疫原:
A synthesized peptide derived from human MYOCD, corresponding to a region within the internal amino acids.
基因/基因ID:
描述:
Transcriptional factor that uses the canonical single or multiple CArG boxes DNA sequence. Binds CArG boxes only in the presence of serum response factor (SRF). Acts as a cofactor of SRF and modulates SRF-target genes. Regulates the expression of a set of cardiac and smooth muscle-specific genes. Plays a crucial role in cardiogenesis and differentiation of the smooth muscle cell lineage.
文献引用
1). MBNL1 regulates isoproterenol‐induced myocardial remodelling in vitro and in vivo. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, 2021
(PubMed: 33295096)
[IF=5.3]
Application: WB Species: mice Sample: cardiomyocytes
FIGURE 3 MBNL1 increases Myocardin expression by binding to UGCU at the 3'-UTR of Myocardin mRNA. A, The 3'-UTR region of
Myocardin contains the binding site of MBNL1. B, The binding of MBNL1 to Myocardin mRNA was validated using RIP. (n = 3, *, P < .05).
C-F, The changes in Myocardin and MBNL1 in cardiomyocytes with overexpressed or silenced MBNL1 were measured using realtime PCR
and Western blotting. (n = 3, *, P < .05, **, P < .01). G and H, Myocardin mRNA residues after 0, 20, 40 and 60 min in cardiomyocytes treated
with Actinomycin D (ACD, 5 μg/mL) and with overexpressed or silenced MBNL1were measured using realtime PCR. I, The 3'-UTR region
of Myocardin mRNA was transcribed in vitro and labelled with biotin. The combination of MBNL1 protein and 3'-UTR of Myocardin was
detected using RNA pulldown assay. J, The RNA probes corresponding to nine prediction sites (probe # 10-18) and their corresponding
antisense probes (probe # 1-9) were synthesized and biotin-labelled. The combination site of MBNL1 protein in the 3'-UTR of Myocardin was
confirmed using RNA pull-down assay
2). LncRNA-Mhrt regulates cardiac hypertrophy by modulating the miR-145a-5p/KLF4/myocardin axis. JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY, 2020
(PubMed: 31982428)
[IF=4.9]
Application: WB Species: mouse Sample: primary cardiomyocytes
Fig. 2. |Mhrt regulates myocardin expression through KLF4 in primary cardiomyocytes.(D, E and F) Following knock down of endogenous KLF4 in primary cardiomyocytes, the effect of Mhrt on myocardin mRNA and protein levels was examined (n = 3, *,P < .05, **, P < .01, #, P > .05).
3). Myocardin Reverses Hypoxia-Inducible Factor-1α Mediated Phenotypic Modulation of Corpus Cavernosum Smooth Muscle Cells in Hypoxia Induced by. World Journal of Mens Health, 2023
(PubMed: 35274501)
[IF=4.0]
4). Myocardin Reverses Hypoxia-Inducible Factor-1α Mediated Phenotypic Modulation of Corpus Cavernosum Smooth Muscle Cells in Hypoxia Induced by Cobalt Chloride. The world journal of men's health, 2023
(PubMed: 35274501)
[IF=4.0]
Application: IF/ICC Species: human Sample: CCSMCs
Fig. 3. Phenotypic modulation in CCSMCs under hypoxia. (A) Representative images of immunofluorescence detecting HIF-1α, Myocd and α-SMA. Scale bar=100 µm. (B) Quantitative results of the statistical analysis. (C) Representative western blotting results of HIF-1α, Myocd, α-SMA, calponin, and OPN. (D) Quantitative results of the statistical analysis. n=3 for each group. CCSMCs: corporal cavernosum smooth muscle cells, HIF-1α: hypoxia-inducible factor-1α, α-SMA: alpha-smooth muscle actin, Myocd: myocardin, OPN: osteopontin. *p
Application: WB Species: human Sample: CCSMCs
Fig. 3. Phenotypic modulation in CCSMCs under hypoxia. (A) Representative images of immunofluorescence detecting HIF-1α, Myocd and α-SMA. Scale bar=100 µm. (B) Quantitative results of the statistical analysis. (C) Representative western blotting results of HIF-1α, Myocd, α-SMA, calponin, and OPN. (D) Quantitative results of the statistical analysis. n=3 for each group. CCSMCs: corporal cavernosum smooth muscle cells, HIF-1α: hypoxia-inducible factor-1α, α-SMA: alpha-smooth muscle actin, Myocd: myocardin, OPN: osteopontin. *p
5). KLHL38 facilitates STS‐induced apoptosis in HL‐1 cells via myocardin degradation. IUBMB LIFE, 2022
(PubMed: 35112472)
[IF=3.7]
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