IREB1 Antibody - #AF6792

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产品: IREB1 抗体
货号: AF6792
描述: Rabbit polyclonal antibody to IREB1
应用: WB
反应: Human, Mouse, Rat
分子量: 80,100kD; 98kD(Calculated).
蛋白号: P21399
RRID: AB_2847515

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   规格 价格 库存
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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实验方案
WB Handbook
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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
克隆:
Polyclonal
特异性:
IREB1 Antibody detects endogenous levels of total IREB1.
RRID:
AB_2847515
引用格式: Affinity Biosciences Cat# AF6792, RRID:AB_2847515.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

ACO 1; ACO1; ACOC_HUMAN; Aconitase 1 soluble; Aconitase; Aconitase1; Aconitate hydratase; ACONS; Citrate hydro lyase; Citrate hydro-lyase; Cytoplasmic aconitate hydratase; Ferritin repressor protein; IRE BP 1; IRE-BP 1; IREB 1; IREB1; IREBP; IREBP1; Iron regulatory protein 1; Iron responsive element binding protein 1; Iron-responsive element-binding protein 1; IRP 1; IRP1; OTTHUMP00000045233;

抗原和靶标

免疫原:

A synthesized peptide derived from human IREB1.

Uniprot:

P21399(ACOC_HUMAN) >>Visit HPA database.

基因/基因ID:
ACO1(48)
序列:
MSNPFAHLAEPLDPVQPGKKFFNLNKLEDSRYGRLPFSIRVLLEAAIRNCDEFLVKKQDIENILHWNVTQHKNIEVPFKPARVILQDFTGVPAVVDFAAMRDAVKKLGGDPEKINPVCPADLVIDHSIQVDFNRRADSLQKNQDLEFERNRERFEFLKWGSQAFHNMRIIPPGSGIIHQVNLEYLARVVFDQDGYYYPDSLVGTDSHTTMIDGLGILGWGVGGIEAEAVMLGQPISMVLPQVIGYRLMGKPHPLVTSTDIVLTITKHLRQVGVVGKFVEFFGPGVAQLSIADRATIANMCPEYGATAAFFPVDEVSITYLVQTGRDEEKLKYIKKYLQAVGMFRDFNDPSQDPDFTQVVELDLKTVVPCCSGPKRPQDKVAVSDMKKDFESCLGAKQGFKGFQVAPEHHNDHKTFIYDNTEFTLAHGSVVIAAITSCTNTSNPSVMLGAGLLAKKAVDAGLNVMPYIKTSLSPGSGVVTYYLQESGVMPYLSQLGFDVVGYGCMTCIGNSGPLPEPVVEAITQGDLVAVGVLSGNRNFEGRVHPNTRANYLASPPLVIAYAIAGTIRIDFEKEPLGVNAKGQQVFLKDIWPTRDEIQAVERQYVIPGMFKEVYQKIETVNESWNALATPSDKLFFWNSKSTYIKSPPFFENLTLDLQPPKSIVDAYVLLNLGDSVTTDHISPAGNIARNSPAARYLTNRGLTPREFNSYGSRRGNDAVMARGTFANIRLLNRFLNKQAPQTIHLPSGEILDVFDAAERYQQAGLPLIVLAGKEYGAGSSRDWAAKGPFLLGIKAVLAESYERIHRSNLVGMGVIPLEYLPGENADALGLTGQERYTIIIPENLKPQMKVQVKLDTGKTFQAVMRFDTDVELTYFLNGGILNYMIRKMAK

翻译修饰 - P21399 作为底物

Site PTM Type Enzyme
K26 Ubiquitination
S30 Phosphorylation
K79 Ubiquitination
K105 Ubiquitination
K106 Ubiquitination
S138 Phosphorylation P17252 (PRKCA)
K141 Ubiquitination
K158 Ubiquitination
Y184 Phosphorylation
K250 Ubiquitination
T258 Phosphorylation
T318 Phosphorylation
K335 Ubiquitination
Y336 Phosphorylation
S383 Phosphorylation
K386 Ubiquitination
K387 Ubiquitination
K396 Ubiquitination
K400 Ubiquitination
K455 Ubiquitination
Y466 Phosphorylation
S533 Phosphorylation
K572 Ubiquitination
K580 Ubiquitination
K587 Ubiquitination
K610 Ubiquitination
K615 Ubiquitination
S622 Phosphorylation
T628 Phosphorylation
K632 Ubiquitination
S638 Phosphorylation
K639 Ubiquitination
K644 Ubiquitination
S645 Phosphorylation
S711 Phosphorylation P17252 (PRKCA)
R713 Methylation
R721 Methylation
K736 Ubiquitination
K772 Ubiquitination
K785 Ubiquitination
S799 Phosphorylation
Y800 Phosphorylation
S806 Phosphorylation
K844 Ubiquitination
K852 Ubiquitination
K857 Ubiquitination

研究背景

功能:

Iron sensor. Binds a 4Fe-4S cluster and functions as aconitase when cellular iron levels are high. Functions as mRNA binding protein that regulates uptake, sequestration and utilization of iron when cellular iron levels are low. Binds to iron-responsive elements (IRES) in target mRNA species when iron levels are low. Binding of a 4Fe-4S cluster precludes RNA binding.

Catalyzes the isomerization of citrate to isocitrate via cis-aconitate.

细胞定位:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Interacts (when associated with the 4Fe-4S) with FBXL5. Interacts with frataxin(81-210).

蛋白家族:

Belongs to the aconitase/IPM isomerase family.

研究领域

· Metabolism > Carbohydrate metabolism > Citrate cycle (TCA cycle).

· Metabolism > Carbohydrate metabolism > Glyoxylate and dicarboxylate metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Carbon metabolism.

· Metabolism > Global and overview maps > 2-Oxocarboxylic acid metabolism.

· Metabolism > Global and overview maps > Biosynthesis of amino acids.

文献引用

1). Alleviated NCOA4-mediated ferritinophagy protected RA FLSs from ferroptosis in lipopolysaccharide-induced inflammation under hypoxia. Inflammation research : official journal of the European Histamine Research Society ... [et al.] (PubMed: 38189810) [IF=6.7]

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