SGK1 Antibody - #AF6789

>>赠JD卡 已结束>>
(1)   (3)  
产品: SGK1 抗体
货号: AF6789
描述: Rabbit polyclonal antibody to SGK1
应用: WB IF/ICC
文献验证: WB
反应: Human, Mouse, Rat, Monkey
蛋白号: O00141
RRID: AB_2847512

浏览相似产品>>

   规格 价格 库存
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

联系销售
相关下载
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
IF/ICC 1:100-1:500, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat, Monkey
克隆:
Polyclonal
特异性:
SGK1 Antibody detects endogenous levels of total SGK1.
RRID:
AB_2847512
引用格式: Affinity Biosciences Cat# AF6789, RRID:AB_2847512.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

OTTHUMP00000017247; Serine/threonine protein kinase SGK; Serine/threonine protein kinase Sgk1; Serine/threonine-protein kinase Sgk1; Serum and glucocorticoid regulated kinase; Serum/glucocorticoid regulated kinase 1; Serum/glucocorticoid regulated kinase; Serum/glucocorticoid-regulated kinase 1; SGK 1; SGK; SGK1; Sgk1 variant i3; SGK1_HUMAN;

抗原和靶标

免疫原:

A synthesized peptide derived from human SGK1.

基因/基因ID:
SGK1(6446)

研究领域

· Environmental Information Processing > Signal transduction > FoxO signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > mTOR signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Organismal Systems > Excretory system > Aldosterone-regulated sodium reabsorption.

文献引用

1). FOSL2 participates in renal fibrosis via SGK1-mediated epithelial-mesenchymal transition of proximal tubular epithelial cells. Journal of Translational Internal Medicine, 2023 (PubMed: 37662889) [IF=4.7]

Application: WB    Species: Human    Sample: HK-2 cells

Figure 7 FOSL2 upregulates SGK1 expression by enhancing the activity of SGK1 transcription. (A, B) The qRT-PCR and western blotting analysis of SGK1 in HK-2 cells after FOSL2 silencing or overexpression. (C) Schematic diagram of four potential FOSL2-binding sites and truncated fragments on the SGK1 promoter. (D) The regulation of FOSL2 on SGK1 transcription activity was evaluated by dual-luciferase assay. (E) A dual-luciferase assay was performed to detect the binding site of FOSL2 on SGK1 promoters. The values are expressed as mean ± standard deviation. **P < 0.01 versus siNC, vector, or control group; ##P < 0.01 versus TGF-β1 + siNC. qRT-PCR: quantitative real-time polymerase chain reaction; TGF-β1: transforming growth factor-β1; SGK1: serum/glucocorticoid regulated kinase 1; FOSL2: fos-related antigen 2.

限制条款

产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）

产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。 

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.