产品: Synaptotagmin 1/2 抗体
货号: AF6223
描述: Rabbit polyclonal antibody to Synaptotagmin 1/2
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat, Monkey
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 65kDa; 48kD,47kD(Calculated).
蛋白号: P21579 | Q8N9I0
RRID: AB_2835040

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat,Monkey
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(80%)
克隆:
Polyclonal
特异性:
Synaptotagmin 1/2 Antibody detects endogenous levels of total Synaptotagmin 1/2.
RRID:
AB_2835040
引用格式: Affinity Biosciences Cat# AF6223, RRID:AB_2835040.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

DKFZp781D2042; FLJ42519; p65; SVP65; Synaptotagmin 2; Synaptotagmin I; Synaptotagmin II; Synaptotagmin-1; SYT; Syt1; SYT1_HUMAN; Syt2; SytI; Synaptotagmin 2; Synaptotagmin II; Synaptotagmin-2; Synaptotagmin2; SynaptotagminII; SYT 2; Syt II; Syt2; SYT2_HUMAN; SytII;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
P21579 SYT1_HUMAN:

Expressed in melanocytes (PubMed:23999003).

Q8N9I0 SYT2_HUMAN:

Expressed in melanocytes (PubMed:23999003).

描述:
SYT2 May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone.
序列:
MVSESHHEALAAPPVTTVATVLPSNATEPASPGEGKEDAFSKLKEKFMNELHKIPLPPWALIAIAIVAVLLVLTCCFCICKKCLFKKKNKKKGKEKGGKNAINMKDVKDLGKTMKDQALKDDDAETGLTDGEEKEEPKEEEKLGKLQYSLDYDFQNNQLLVGIIQAAELPALDMGGTSDPYVKVFLLPDKKKKFETKVHRKTLNPVFNEQFTFKVPYSELGGKTLVMAVYDFDRFSKHDIIGEFKVPMNTVDFGHVTEEWRDLQSAEKEEQEKLGDICFSLRYVPTAGKLTVVILEAKNLKKMDVGGLSDPYVKIHLMQNGKRLKKKKTTIKKNTLNPYYNESFSFEVPFEQIQKVQVVVTVLDYDKIGKNDAIGKVFVGYNSTGAELRHWSDMLANPRRPIAQWHTLQVEEEVDAMLAVKK

MRNIFKRNQEPIVAPATTTATMPIGPVDNSTESGGAGESQEDMFAKLKEKLFNEINKIPLPPWALIAIAVVAGLLLLTCCFCICKKCCCKKKKNKKEKGKGMKNAMNMKDMKGGQDDDDAETGLTEGEGEGEEEKEPENLGKLQFSLDYDFQANQLTVGVLQAAELPALDMGGTSDPYVKVFLLPDKKKKYETKVHRKTLNPAFNETFTFKVPYQELGGKTLVMAIYDFDRFSKHDIIGEVKVPMNTVDLGQPIEEWRDLQGGEKEEPEKLGDICTSLRYVPTAGKLTVCILEAKNLKKMDVGGLSDPYVKIHLMQNGKRLKKKKTTVKKKTLNPYFNESFSFEIPFEQIQKVQVVVTVLDYDKLGKNEAIGKIFVGSNATGTELRHWSDMLANPRRPIAQWHSLKPEEEVDALLGKNK

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
80
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P21579/Q8N9I0 作为底物

Site PTM Type Enzyme
S24 Phosphorylation
K120 Ubiquitination
T126 Phosphorylation
T129 Phosphorylation
K134 Ubiquitination
Y181 Phosphorylation
T202 Phosphorylation
T212 Phosphorylation
Y217 Phosphorylation
Y230 Phosphorylation
K237 Acetylation
K302 Acetylation
Y312 Phosphorylation
K333 Ubiquitination
Site PTM Type Enzyme
K46 Acetylation
K48 Acetylation
K98 Ubiquitination
T122 Phosphorylation
T125 Phosphorylation
Y178 Phosphorylation
T199 Phosphorylation Q9H4A3 (WNK1)
K299 Acetylation
Y309 Phosphorylation
S378 Phosphorylation
T381 Phosphorylation
T383 Phosphorylation Q9H4A3 (WNK1)

研究背景

功能:

Calcium sensor that participates in triggering neurotransmitter release at the synapse (By similarity). May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse (By similarity). It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2. Plays a role in dendrite formation by melanocytes.

翻译修饰:

Glycosylated.

细胞定位:

Cytoplasmic vesicle>Secretory vesicle membrane>Single-pass membrane protein. Cytoplasmic vesicle>Secretory vesicle>Synaptic vesicle membrane>Single-pass membrane protein. Cytoplasmic vesicle>Secretory vesicle>Chromaffin granule membrane>Single-pass membrane protein. Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in melanocytes.

亚基结构:

Homotetramer (Probable). Interacts with SCAMP5. Interacts with STON2. Forms a complex with SV2B, syntaxin 1 and SNAP25 (By similarity). Interacts with SV2A, SV2B and SV2C (By similarity). Interacts with RIMS1 (By similarity). Interacts with PRRT2 (By similarity). Interacts with DNAJC5 in a phosphorylation-dependent manner (By similarity). Interacts (via N-terminus) with RAB3A (By similarity). Interacts with SYT12 (By similarity). Interacts with calmodulin (By similarity).

蛋白家族:

The first C2 domain mediates Ca(2+)-dependent phospholipid binding.

The second C2 domain mediates interaction with SV2A and probably with STN2.

Belongs to the synaptotagmin family.

功能:

Exhibits calcium-dependent phospholipid and inositol polyphosphate binding properties (By similarity). May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse (By similarity). Plays a role in dendrite formation by melanocytes.

细胞定位:

Cytoplasmic vesicle>Secretory vesicle>Synaptic vesicle membrane>Single-pass membrane protein. Cytoplasmic vesicle>Secretory vesicle>Chromaffin granule membrane>Single-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in melanocytes.

亚基结构:

Homotetramer (Probable). Interacts with STON2. Interacts with SCAMP5. Interacts with PRRT2 (By similarity).

蛋白家族:

The first C2 domain mediates Ca(2+)-dependent phospholipid binding.

The second C2 domain mediates interaction with Stonin 2. The second C2 domain mediates phospholipid and inositol polyphosphate binding in a calcium-independent manner.

Belongs to the synaptotagmin family.

研究领域

· Organismal Systems > Nervous system > Synaptic vesicle cycle.

文献引用

1). Sisi Li et al. Fibroblast growth factor 2 contributes to the effect of salidroside on dendritic and synaptic plasticity after cerebral ischemia/reperfusion injury. Aging (Albany NY) 2020 Jun 9;12. (PubMed: 32518214) [IF=5.2]

Application: WB    Species: rat    Sample:

Figure 10.| Sal promotes increases in dendritic spine density and synaptic-associated protein expression via upregulating the FGF2-mediated cAMP/PKA/CREB signaling pathway.(E–H) Representative western blot bands of PSD95, synapsin I and synaptotagmin in each group. (I) QPCR data for PSD95. (J) Transmission electron microscopy showed the synaptic structures (the scale bar is 1 μm). Values are expressed as the mean ± SD. #p < 0.05, ##p < 0.01 vs. sham; *p < 0.05, **p < 0.01 vs. MCAO/R.

2). Qiaoyun Wu et al. Electroacupuncture may alleviate neuropathic pain via suppressing P2X7R expression. Molecular Pain Jan-Dec 2021;17:1744806921997654. (PubMed: 33626989) [IF=3.3]

Application: WB    Species: rats    Sample: spinal dorsal horn

Figure 2. EA reduced abnormal remodeling of dendritic spines/synapses, whereas BzATP had the opposite effects. (a) and (b) Representative Western blots and quantification data of the expression of Synaptotagmin 1/GAPDH. (n ¼ 3). (c) Dendritic spines. Scale bars, 10 lm. (d) Number of spines per 10 lm. (n ¼ 3). (e) The ultrastructure of synapses in the spinal dorsal horn from rats in each group (0.2 lm, 30,000 ). (f) Number of synapses per 30000x field. (n ¼ 4). (g) Width of synaptic space (nm). (n ¼ 4). (h) Vesicle density (vesicles/ mm2 ). (n ¼ 4). Columns represent the mean  SD. #p < 0.05 and ##p < 0.01 vs. the S group; $p < 0.05 and $$p < 0.01 vs. the M group; &&p < 0.01 vs. the MþEA group.

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