GFAP Antibody - #AF6166

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产品: GFAP 抗体
货号: AF6166
描述: Rabbit polyclonal antibody to GFAP
应用: WB
文献验证: WB
反应: Human, Mouse, Rat
预测: Bovine, Horse
分子量: 50kDa; 50kD(Calculated).
蛋白号: P14136
RRID: AB_2835032

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MSDS
说明书
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
预测:
Bovine(100%), Horse(%)
克隆:
Polyclonal
特异性:
GFAP Antibody detects endogenous levels of total GFAP.
RRID:
AB_2835032
引用格式: Affinity Biosciences Cat# AF6166, RRID:AB_2835032.
偶联:
Unconjugated. 130
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

wu:fb34h11; ALXDRD; cb345; etID36982.3; FLJ42474; FLJ45472; GFAP; GFAP_HUMAN; gfapl; Glial fibrillary acidic protein; Intermediate filament protein; wu:fk42c12; xx:af506734; zgc:110485;

抗原和靶标

免疫原:
Uniprot:

P14136(GFAP_HUMAN) >>Visit HPA database.

基因/基因ID:
GFAP(2670)
表达:
P14136 GFAP_HUMAN:

Expressed in cells lacking fibronectin.

描述:
This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. An additional transcript variant has been described, but its full length sequence has not been determined.
序列:
MERRRITSAARRSYVSSGEMMVGGLAPGRRLGPGTRLSLARMPPPLPTRVDFSLAGALNAGFKETRASERAEMMELNDRFASYIEKVRFLEQQNKALAAELNQLRAKEPTKLADVYQAELRELRLRLDQLTANSARLEVERDNLAQDLATVRQKLQDETNLRLEAENNLAAYRQEADEATLARLDLERKIESLEEEIRFLRKIHEEEVRELQEQLARQQVHVELDVAKPDLTAALKEIRTQYEAMASSNMHEAEEWYRSKFADLTDAAARNAELLRQAKHEANDYRRQLQSLTCDLESLRGTNESLERQMREQEERHVREAASYQEALARLEEEGQSLKDEMARHLQEYQDLLNVKLALDIEIATYRKLLEGEENRITIPVQTFSNLQIRETSLDTKSVSEGHLKRNIVVKTVEMRDGEVIKESKQEHKDVM

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Horse
100
Bovine
100
Pig
0
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

功能:

GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.

翻译修饰:

Phosphorylated by PKN1.

细胞定位:

Cytoplasm.
Note: Associated with intermediate filaments.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in cells lacking fibronectin.

亚基结构:

Interacts with SYNM (By similarity). Isoform 3 interacts with PSEN1 (via N-terminus).

蛋白家族:

Belongs to the intermediate filament family.

研究领域

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway.   (View pathway)

文献引用

1). The effects of exosomes originating from different cell sources on the differentiation of bone marrow mesenchymal stem cells into schwann cells. Journal of nanobiotechnology, 2024 (PubMed: 38698449) [IF=10.2]

Application: WB    Species: Rat    Sample: BMSCs

Fig. 2 Protein expression of Schwann cell markers in differentiallyinduced BMSCs(n = 3). (A) Representative immunofluorescence micrographs showing the expression levels of Schwann cell markers (S100, GFAP, P75NGRF) in twelve experimental groups (BMSCs, BMSCs + ODM, BMSCs + RSC96-exos, BMSCs + Fb-exos, BMSCs + NSC-exos, BMSCs + ODM + RSC96-exos, BMSCs + ODM + Fb-exos, BMSCs + ODM + NSC-exos, induced BMSCs + RSC96-exos, induced BMSCs + Fb-exo, induced BMSCs + NSC-exo and RSC96) (magnification, x100). Scale bar, 50 μm. (B) Representative western blotting of the protein expression levels of S100, GFAP, and P75NGRF in the twelve groups

Application: IF/ICC    Species: Rat    Sample: BMSCs

Fig. 2 Protein expression of Schwann cell markers in differentiallyinduced BMSCs(n = 3). (A) Representative immunofluorescence micrographs showing the expression levels of Schwann cell markers (S100, GFAP, P75NGRF) in twelve experimental groups (BMSCs, BMSCs + ODM, BMSCs + RSC96-exos, BMSCs + Fb-exos, BMSCs + NSC-exos, BMSCs + ODM + RSC96-exos, BMSCs + ODM + Fb-exos, BMSCs + ODM + NSC-exos, induced BMSCs + RSC96-exos, induced BMSCs + Fb-exo, induced BMSCs + NSC-exo and RSC96) (magnification, x100). Scale bar, 50 μm. (B) Representative western blotting of the protein expression levels of S100, GFAP, and P75NGRF in the twelve groups
2). Neurogenesis promoted by the CD200/CD200R signaling pathway following treadmill exercise enhances post-stroke functional recovery in rats. BRAIN BEHAVIOR AND IMMUNITY, 2019 (PubMed: 31513876) [IF=8.8]

Application: IF/ICC    Species: rat    Sample: astrocytes

Fig. 5.| The expression of CD200 and CD200R was up-regulated induced by treadmill exercise after stroke in rats.The effect of treadmill exercise on the protein levels of CD200 and CD200R in the ischemic cortex (A-C) and hippocampus (D-F) at 7 and 28 days after tMCAO. CD200R staining shows protein expression at 7 and 28 days after treadmill exercise post-stroke (G). CD200R colocalizes with activated microglia (Iba1+cells, white arrows), not colocalizes with neural stem cells (Nestin+ cells) and astrocytes (GFAP+ cells), Scale bar: 50 μm. N = 4. Results are showed as means ± SEM. # P < 0.05, ## P < 0.01, ### P < 0.001 versus the Sham group. *P < 0.05, ** P < 0.01, *** P < 0.001 versus the tMCAO group.
3). Design, synthesis and bioactivity study of N-salicyloyl tryptamine derivatives as multifunctional agents for the treatment of neuroinflammation. EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY, 2020 (PubMed: 32182488) [IF=6.0]

Application: IF/ICC    Species: Mouse    Sample: BV2 and C6 cells

Fig. 4. Effects of compounds on GFAP and Iba-1 expression in the LPS-induced astrocytes (C6, treated with 10 mg/mL of LPS) and microglia (BV2, treated with 1 mg/mL of LPS). A. Representative fluorescence microscopy images of GFAP immunostaining (Magnification  20). B. Representative fluorescence microscopy images of Iba-1 immunostaining (Magnification  40). C. Histograms show relative changes of GFAP expressed as mean ± SD of the three independent experiments (n ¼ 3). Ctrl. ¼ Control, LPS ¼ Lipopolysaccharide (10 mg/mL); 3, 13, 16 ¼ treatments by compounds 3, 13, 16 (20 mM) along with LPS (10 mg/mL). D. Histograms show relative changes of Iba-1 expressed as mean ± SD of the three independent experiments (n ¼ 3). All the data were analyzed using Image Pro Plus and expressed as a percent of LPS group values (fluorescence intensity). (#) Significant difference (##p < 0.01, ###p < 0.001) vs. control and (*) significant difference (*p < 0.05 and **p < 0.01) vs. LPS.
4). Erythropoietin-Induced Autophagy Protects Against Spinal Cord Injury and Improves Neurological Function via the Extracellular-Regulated Protein Kinase Signaling Pathway. MOLECULAR NEUROBIOLOGY, 2020 (PubMed: 32647973) [IF=4.6]

Application: IF/ICC    Species: rat    Sample:

Fig. 6 |EPO benefit antiinflammation and axonal regeneration can be neutralized by ERK inhibition, and activator can mimic the effect of EPO. a The axonal special protein GAP43 immunofluorescence staining and histogram at 14 days (magnification × 400). b–e The inflammatory marker proteins immunofluorescence staining and histograms at 14 days, including CD86, GFAP, TNF-α, iNOS(magnification × 400). All data presented as mean ± SEM in each group. *p < 0.05 vs. SCI + saline group, **p < 0.01 vs. SCI + saline group. #p < 0.05 comparison between SCI + EPO group and SCI + EPO + PD98059, ##p < 0.01 comparison between SCI + EPO group and SCI + EPO + PD98059
5). Effect of Shenqi Jieyu formula on inflammatory response pathway in hippocampus of postpartum depression rats. Heliyon, 2024 (PubMed: 38726147) [IF=4.0]

Application: IHC    Species: Rat    Sample:

Fig. 11 Protein expression of GFAP‾(χ±s, n = 3) in hippocampi of rats in each group (200 × ). Protein expression of GFAP increased in PPD animals. SJF, AUDA, and paroxetine hydrochloride reversed the changes in protein expression of GFAP.
6). Synaptic Plasticity of Human Umbilical Cord Mesenchymal Stem Cell Differentiating into Neuron-like Cells In Vitro Induced by Edaravone. Stem Cells International, 2018 (PubMed: 30510585) [IF=3.8]

Application: WB    Species: human    Sample: neuron-like cells

Figure 8:| (a) Result of Western blot. (b) Relative expression level of protein.
7). Stem Cells in Cancer Progression and Therapy. Stem Cells International, 2019 (PubMed: 30949210) [IF=3.8]
8). Schwann cell‑derived exosomes induce bone marrow‑derived mesenchymal stem cells to express Schwann cell markers in vitro. Molecular Medicine Reports, 2020 (PubMed: 32016464) [IF=3.4]

Application: WB    Species: rat    Sample: BMSCs

Figure 3. |mRNA and protein expression levels of Schwann cell markers in differentially‑induced BMSCs. (F and G) Representative immunoblot of protein expression levels of S100, GFAP, NGRF, Sox10, EGR2 and GAPDH in control BMSCs, induced BMSCs, BMSCs + RSC96 exo, BMSCs + Fb exo or RSC96 cells. Data are presented as mean ± SD; *P<0.05, **P<0.01 and ***P<0.001. BMSCs, bone marrow mesenchymal stem cells; EGR2, early growth response 2; Fb exo, fibroblast‑derived exosomes; GFAP, glial fibrillary acidic protein; induced BMSCs, Dezawa's induction method; NGFR, low‑affinity nerve growth factor receptor; RSC96 exo, Schwann cell‑derived exosomes.
9). Carbenoxolone has the potential to ameliorate acute incision pain in rats. Molecular Medicine Reports, 2021 (PubMed: 34013377) [IF=3.4]

Application: IF/ICC    Species: Rat    Sample:

Figure 3. Co-localization of Px1 and GFAP in IP model rats. Px1 (green) and GFAP (red) expression was analyzed using an immunofluorescence assay. Magnification, ×200; scale bar, 10 µm. C, control group; IP, incision pain; CBX, carbenoxolone; 10panx, pannexin-1 mimetic inhibitory peptide; Px1, pannexin 1; GFAP, glial fibrillary acidic protein.
10). Mitochondrial apoptosis and autophagy in the process of adipose-derived stromal cell differentiation into astrocytes. CELL BIOLOGY INTERNATIONAL, 2016 (PubMed: 26399453) [IF=3.3]
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