Vitamin D Receptor Antibody - #AF6159

Fig. 7 Eriodictyol up-regulates VDR expression and activates the Nrf2/HO-1 signaling pathway. A VDR expression in the mouse brain was detected using immunohistochemical staining. Then, B the VDR protein expression level in the mouse cortex and hippocampus was measured using Western blotting. C The VDR protein expression level in HT-22 cells was measured using Western blotting, and D its relative mRNA expression level was detected using q-PCR. E The levels of Nrf2, p-Nrf2 and HO-1 in the mouse cortex and hippocampus were measured using Western blotting. F The levels of Nrf2, p-Nrf2 and HO-1 in HT-22 cells were measured using Western blotting. G The level of Nrf2 in the nucleus and cytoplasm of HT-22 cells was detected using Western blotting, and Lamin B1 and β-actin were used as loading controls for the nuclear and cytoplasmic proteins, respectively. The data are presented as the means ± SD of three experiments. **P < 0.01 and ***P < 0.001 compared with the Aβ1–42 oligomer group. ##P < 0.01 compared with the control group

FIGURE 2 PTS reduces reduction of NLRP3 inflammasome and apoptosis in DOX-treated mice. (A) WT C57/BL6J mice were pretreated with PTS (10 mg/kg) and afterwards with DOX (20 mg/kg cumulative dose) for 6 days. Immunochemistry staining of the liver sections with anti-NLRP3 (left, scale bar = 50 μm), the quantification of NLRP3 positive area in each group (right, n = 6); (B) qPCR analyses of NLRP3, IL-1β and IL-18 mRNA levels in each group (n = 6); (C) Western blot analyses of NLRP3, ASC, Caspase-1 p20, IL-1β, and IL-18 proteins and the quantification of the blots in each group (n = 4 per group); (D) Western blot analyses of Cleaved Caspase-3, BAX, and BCL-2 proteins (left) and quantification of the blots in each group (right, n = 4 per group).

Figure 1 Expression of LAT1 and VDR in normal and preeclamptic placentas. (A) Representative immunostaining images of LAT1 and VDR expressions in tissue sections from normal and preeclamptic (PE) placentas. Bar = 100 micron. (B) Representative blots of LAT1 and VDR expression in placenta from normotensive and PE pregnancies. (C,D) The bar graphs show the relative density of protein expression for LAT1 and VDR after normalization with β-actin expression in each sample. n = 5, #P < 0.05, PE vs Normal.

Figure 2 Effects of 1,25(OH)2D3 and CoCl2 on LAT1 and VDR expression in placental trophoblasts. (A) Protein expression for LAT1 and VDR in HTR-8/SVneo trophoblast cells treated with different concentrations of CoCl2. The bar graphs show relative protein expression for LAT1 and VDR after normalized against β-actin in each sample from three independent experiments. CoCl2 induced a dose-dependent decrease in both LAT1 and VDR expression in trophoblasts. *P < 0.05, **P < 0.01, ***P < 0.001, CoCl2 treated vs control cells. (B) Protein expression for LAT1 and VDR in HTR-8/SVneo trophoblast cells treated with different concentrations of 1,25(OH)2D3. The bar graphs show relative protein expression for LAT1 and VDR after normalization with β-actin in each sample from four independent experiments. In contrast to CoCl2, 1,25(OH)2D3 stimulated LAT1 and VDR expression in a dose-dependent manner in trophoblast cells. #P < 0.05, ##P < 0.01, 1,25(OH)2D3 treated vs control cells.

Figure 3.| Efects of 1,25(OH)2D3 on CoCl2-induced LAT1 expression in placental trophoblasts. (C) Protein expression of VDR and LAT1 expression in trophoblasts treated with VDR siRNA in the presence or absence of 1,25(OH)2D3. Inhibition of VDR expression attenuates 1,25(OH)2D3-stimulated LAT1 expression in trophoblast cells. Te bar graphs show relative VDR and LAT1 expression afer being normalized by β-actin in each sample from six independent experiments, *P<0.05, **P<0.01, treated vs control; ##P<0.01, ###P<0.001, 1,25(OH)2D3+VDR siRNA vs 1,25(OH)2D3 alone.

Figure 1.| Representative VDR, COX-2, and miR-26b-5p expression in placenta and trophoblasts from normotensive pregnant women and women complicated with preeclampsia (PE). (A) VDR expression and COX-2 expression in placental tissues from 12 pregnant women (6 from normotensive and 6 from PE). Strong VDR staining was seen in the placentas from normotensive pregnant women compared to those from women with PE. In contrast, intensive COX-2 expression was detected in placentas from PE specimens. Bar=100 micron.

Figure 3. Wnt5A/B upregulates YAP in OA-CSPCs. a The mRNA expression of the genes associated with stem cell function in OA-CSPCs. b The protein levels of YAP, FAP, VDR, TERT, and MMP3 in OA-CSPCs. c Immunofluorescence staining of YAP (red) in OA-CSPCs. Nuclei were stained by 4,6-diamidina-2-phenylin (DAPI,blue), Bars=100 μm. d The protein levels of YAP in OA-CSPCs derived from OA patients.