c-Kit Antibody - #AF6153

>>买2送1 进行中>>
>>赠JD卡 进行中>>
(7)   (33)  
产品: c-Kit 抗体
货号: AF6153
描述: Rabbit polyclonal antibody to c-Kit
应用: WB IHC IF/ICC
文献验证: WB, IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Rabbit, Dog
蛋白号: P10721
RRID: AB_2835024

浏览相似产品>>

   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

联系销售
相关下载
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
c-Kit Antibody detects endogenous levels of total c-Kit.
RRID:
AB_2835024
引用格式: Affinity Biosciences Cat# AF6153, RRID:AB_2835024.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

C Kit; c-Kit; c-Kit Ligand; CD117; Kit; Kit Ligand; KIT oncogene; KIT proto oncogene receptor tyrosine kinase; KIT_HUMAN; Mast cell growth factor receptor; Mast/stem cell growth factor receptor Kit; MGF; p145 c-kit; PBT; Piebald trait protein; Proto oncogene c Kit; Proto oncogene tyrosine protein kinase Kit; Proto-oncogene c-Kit; SCF Receptor; SCFR; soluble KIT variant 1; Steel Factor Receptor; Stem cell factor receptor; tyrosine protein kinase Kit; Tyrosine-protein kinase Kit; v kit Hardy Zuckerman 4 feline sarcoma viral oncogene homolog; v kit Hardy Zuckerman 4 feline sarcoma viral oncogene like protein; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog;

抗原和靶标

免疫原:

A synthesized peptide derived from human c-Kit, corresponding to a region within the internal amino acids.

基因/基因ID:
KIT(3815)
描述:
KIT encodes the human homolog of the proto-oncogene c-kit. C-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. KIT is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Mutations in KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism.

研究领域

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Acute myeloid leukemia.   (View pathway)

· Human Diseases > Cancers: Specific types > Breast cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Central carbon metabolism in cancer.   (View pathway)

· Organismal Systems > Immune system > Hematopoietic cell lineage.   (View pathway)

· Organismal Systems > Endocrine system > Melanogenesis.

文献引用

1). Microbiota-derived H2S induces c-kit+ cDC1 autophagic cell death and liver inflammation in metabolic dysfunction-associated steatohepatitis. Nature communications, 2025 (PubMed: 40044736) [IF=16.6]
2). Upregulation of BCL-2 by acridone derivative through gene promoter i-motif for alleviating liver damage of NAFLD/NASH. NUCLEIC ACIDS RESEARCH, 2020 (PubMed: 32710621) [IF=16.6]

Application: WB    Species: human    Sample: HepG2

Figure 3. Effect of A22 on gene transcription and translation in HepG2 cells. The mRNA levels of BCL-2 and BAX (A), as well as C-KIT, KRAS, C-MYC and VEGF (B) in HepG2 cells were analyzed by using qRT-PCR after incubation with increasing concentration of A22 for 12 h. (C) Effects of A22 on protein expressions of C-MYC, VEGF, C-KIT and BCL-2 in the presence or absence of increasing concentration of A22 for 24 h, which were quantitatively analyzed (D).
3). Identification and validation of immune and diagnostic biomarkers for interstitial cystitis/painful bladder syndrome by integrating bioinformatics and machine-learning. Frontiers in immunology, 2025 (PubMed: 39917301) [IF=5.7]
4). Effects of icariin as a feed additive on the reproductive function in bucks (Capra hircus). Frontiers in veterinary science, 2024 (PubMed: 39575435) [IF=3.2]

Application: IF/ICC    Species: goat    Sample:

Figure 5. Effect of ICA administration on male germ cell markers of dairy goats. (A) Confocal microscopy analyzes the distribution of PGP9.5, DDX4, and c-Kit in the testes. (a) Immunofluorescence analysis showed the expression of PGP9.5 and DDX4. (b) The fluorescence intensity was analyzed on the yellow lines by Fiji software. (c) Immunofluorescence analysis showed the distribution of PGP9.5 and c-Kit. (d) The fluorescence intensity was analyzed on the yellow lines by Fiji software. (B) qPCR and western blot analyze the expression of mRNA and proteins. (a) The mRNA levels of PGP9.5, DDX4, and c-Kit were examined by qPCR. (b) Western blot analysis was performed to measure the expression of target proteins. β-actin was utilized as a loading control. (c) The histogram represents the quantification of proteins levels. Each value represents the mean ± SEM, **p 
5). Organoid models derived from patients with malignant phyllodes tumor of the breast. Breast Cancer Research and Treatment, 2023 (PubMed: 37204665) [IF=3.0]
6). Arctiin alleviates functional constipation by enhancing intestinal motility in mice. Experimental and Therapeutic Medicine, 2023 (PubMed: 37090075) [IF=2.4]
7). Cytological and spectroscopic characteristics of c‐KIT N822K mutation in core binding factor acute myeloid leukemia cells. Journal of Biophotonics, 2020 (PubMed: 32390317) [IF=2.0]

Application: WB    Species: human    Sample: AML cells

Figure 6. |The effects of genetic abnormality-induced cytological and spectral alterations in AML cells. (a) Ratio of clone formation. (b) Relative contents of Raman bands screened by CRT algorithm. (c) Western blot analysis showed the c-KIT and p-c-KIT expression, GAPDH served as loading control, along with (d) ratio of phosphorylated c-KIT to total c-KIT. *P<0.05.

限制条款

产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）

产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。 

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.