TGF alpha Antibody - #AF0262

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产品: TGF alpha 抗体
货号: AF0262
描述: Rabbit polyclonal antibody to TGF alpha
应用: WB IHC IF/ICC
文献验证: IF/ICC
反应: Human, Mouse, Rat, Monkey
预测: Pig, Bovine, Horse
蛋白号: P01135
RRID: AB_2833436

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   规格 价格 库存
 50ul RMB¥ 1000 1250 现货
 100ul RMB¥ 1840 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat, Monkey
克隆:
Polyclonal
特异性:
TGF alpha Antibody detects endogenous levels of total TGF alpha.
RRID:
AB_2833436
引用格式: Affinity Biosciences Cat# AF0262, RRID:AB_2833436.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

EGF like TGF; EGF-like TGF; ETGF; TFGA; TGF A; TGF type 1; TGF-alpha; Tgfa; TGFA_HUMAN; Transforming growth factor alpha; Transforming growth factor alpha precursor; Wa1; Waved 1;

抗原和靶标

免疫原:

A synthesized peptide derived from human TGF alpha, corresponding to a region within C-terminal amino acids.

基因/基因ID:
TGFA(7039)
描述:
TGFalpha TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.

研究领域

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > ErbB signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Colorectal cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Renal cell carcinoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Pancreatic cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Glioma.   (View pathway)

· Human Diseases > Cancers: Specific types > Prostate cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Non-small cell lung cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma.   (View pathway)

· Organismal Systems > Endocrine system > Estrogen signaling pathway.   (View pathway)

文献引用

1). Germacrone Ameliorates Acute Lung Injury Induced by Intestinal Ischemia Reperfusion Via Regulating Macrophage M1 Polarization and Mitochondrial Defects. Acta biochimica et biophysica Sinica, 2024 (PubMed: 39439416) [IF=3.3]

Application: IF/ICC    Species: Mouse    Sample: lung tissues

Figure2 Germacrone attenuated the release of inflammatory factors and inhibits apoptosis. (AD) The mRNA expression levels of IL-1α (A), IL-6 (B), COX2 (C) and TGF-α (D) were determined by RT-qPCR in each group, respectively. (E-F) Immunofluorescence staining of ICAM1 (E) and TGF-α (F) in lung tissues of each group(400x), respectively. (G) TUNEL staining was employed to detect apoptosis level (400x). (H) IHC staining of CD86, CD163 and Gr-1 in lung tissues of each group. (I) The protein expression levels of IL-1β, NOS2, TLR2, CD86, CD115, CD206, ARG1, and CD163 were detected by Western blot in each group, respectively. Sham: control group, I/R+Con: chloral hydrate treated group, I/R+Ger: chloral hydrate treated group with Germacone administration. Data were shown as the mean ± SD.
2). Germacrone ameliorates acute lung injury induced by intestinal ischemia-reperfusion by regulating macrophage M1 polarization and mitochondrial defects. Acta biochimica et biophysica Sinica, 2024 (PubMed: 39439416) [IF=3.3]

Application: IF/ICC    Species: Mouse    Sample: lung tissues

Figure 2 . Germacrone attenuated the release of inflammatory factors and inhibited apoptosis (A‒D) The mRNA expression levels of IL-1α (A), IL-6 (B), COX2 (C), and TGF-α (D) in each group were determined by qPCR. (E,F) Immunofluorescence staining of ICAM1 (E) and TGF-α (F) in lung tissues from each group. Scale bar: 20 μm. (G) TUNEL staining showing the degree of apoptosis. Scale bar: 20 μm. (H) IHC staining of CD86, CD163, and Gr-1 in lung tissues from each group. Scale bar: 20 μm. (I) Protein expression levels of IL-1β, NOS2, TLR2, CD86, CD115, CD206, ARG1, and CD163 in each group, as detected by western blot analysis. Sham: control group; I/RI: mice whose superior mesenteric artery was completely clamped; I/RI + Ger: I/RI group administered with germacrone. Data are shown as the mean ± SD. *P 

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