c-Myb Antibody - #AF6136

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产品: c-Myb 抗体
货号: AF6136
描述: Rabbit polyclonal antibody to c-Myb
应用: WB IF/ICC
反应: Human, Mouse
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 80kDa; 72kD(Calculated).
蛋白号: P10242
RRID: AB_2835019

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
预测:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
c-Myb Antibody detects endogenous levels of total c-Myb.
RRID:
AB_2835019
引用格式: Affinity Biosciences Cat# AF6136, RRID:AB_2835019.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

C myb; C myb protein; Cmyb; MYB; Myb proto oncogene; V myb myeloblastosis viral oncogene homolog;

抗原和靶标

免疫原:
Uniprot:

P10242(MYB_HUMAN) >>Visit HPA database.

基因/基因ID:
MYB(4602)
描述:
Myb is a transcriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells.
序列:
MARRPRHSIYSSDEDDEDFEMCDHDYDGLLPKSGKRHLGKTRWTREEDEKLKKLVEQNGTDDWKVIANYLPNRTDVQCQHRWQKVLNPELIKGPWTKEEDQRVIELVQKYGPKRWSVIAKHLKGRIGKQCRERWHNHLNPEVKKTSWTEEEDRIIYQAHKRLGNRWAEIAKLLPGRTDNAIKNHWNSTMRRKVEQEGYLQESSKASQPAVATSFQKNSHLMGFAQAPPTAQLPATGQPTVNNDYSYYHISEAQNVSSHVPYPVALHVNIVNVPQPAAAAIQRHYNDEDPEKEKRIKELELLLMSTENELKGQQVLPTQNHTCSYPGWHSTTIADHTRPHGDSAPVSCLGEHHSTPSLPADPGSLPEESASPARCMIVHQGTILDNVKNLLEFAETLQFIDSFLNTSSNHENSDLEMPSLTSTPLIGHKLTVTTPFHRDQTVKTQKENTVFRTPAIKRSILESSPRTPTPFKHALAAQEIKYGPLKMLPQTPSHLVEDLQDVIKQESDESGIVAEFQENGPPLLKKIKQEVESPTDKSGNFFCSHHWEGDSLNTQLFTQTSPVADAPNILTSSVLMAPASEDEDNVLKAFTVPKNRSLASPLQPCSSTWEPASCGKMEEQMTSSSQARKYVNAFSARTLVM

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P10242 作为底物

Site PTM Type Enzyme
S11 Phosphorylation P68400 (CSNK2A1)
S12 Phosphorylation P68400 (CSNK2A1)
K53 Ubiquitination
Y69 Phosphorylation
K84 Ubiquitination
K92 Ubiquitination
K97 Ubiquitination
K109 Ubiquitination
K113 Ubiquitination
S116 Phosphorylation P17612 (PRKACA)
K120 Ubiquitination
C130 S-Nitrosylation
K143 Sumoylation
K143 Ubiquitination
K160 Ubiquitination
K171 Ubiquitination
K182 Ubiquitination
K192 Ubiquitination
K204 Ubiquitination
K291 Ubiquitination
K293 Ubiquitination
K296 Ubiquitination
T420 Phosphorylation
T433 Phosphorylation
K442 Ubiquitination
K445 Acetylation
K445 Ubiquitination
T452 Phosphorylation
R457 Methylation
S463 Phosphorylation
R465 Methylation
T466 Phosphorylation
T468 Phosphorylation
K471 Acetylation
K471 Methylation
K471 Ubiquitination
K480 Acetylation
K480 Sumoylation
K480 Ubiquitination
K485 Acetylation
K485 Ubiquitination
K503 Sumoylation
K527 Sumoylation
S532 Phosphorylation P27361 (MAPK3)
T534 Phosphorylation
K593 Ubiquitination
S599 Phosphorylation
K615 Ubiquitination
T621 Phosphorylation
S624 Phosphorylation
K628 Ubiquitination

研究背景

功能:

Transcriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells.

翻译修饰:

Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.

Phosphorylated by NLK on multiple sites, which induces proteasomal degradation.

Phosphorylated by HIPK1. This phosphorylation reduces MYB transcription factor activity but not MYB protein levels.

细胞定位:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Binds MYBBP1A. Interacts with HIPK2, MAF and NLK (By similarity). Binds to HIPK1.

蛋白家族:

Comprised of 3 domains; an N-terminal DNA-binding domain, a centrally located transcriptional activation domain and a C-terminal domain involved in transcriptional repression.

研究领域

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

文献引用

1). LncRNA AK023391 promotes tumorigenesis and invasion of gastric cancer through activation of the PI3K/Akt signaling pathway. Journal of Experimental & Clinical Cancer Research, 2017 (PubMed: 29282102) [IF=11.3]

Application: WB    Species: human    Sample: Gastric cancer cells

Fig. 8 | LncRNA AK023391 was involved in the regulation of the PI3K/Akt signaling pathway.e-f Western blotting validation of the effects of AK023391 knockdown on the expression of PI3K/Akt, NF-κB, p53, and FOXO3a pathways, and their downstream transcription factors c-myb, cyclinB1/G2, and BCL-6 in HGC 27, AGS, and SGC-7901 cells

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