Foxp3 Antibody - #AF6544

Figure 3 Lacidipine exerts immunomodulatory effects in vivo. A) Effect of lacidipine on the proliferation of T cells co-cultured with IFN γ-treated MDA-MB-231. The proliferation of CD8+ T cells was detected based on CFSE using flow cytometry (FACS). The percentage of proliferating cells is shown. The experiments were conducted with three independent replicates. B,C) The expression of CD8 and Foxp3 were examined by immunohistochemical analysis in tumor tissues (n = 6, each group). Representative images are shown. Scale bar = 250 µm. D) Percentage of CD8+ effector T cells in CD45+TILs in tumors was analyzed using flow cytometry at the end of treatment (n = 6, each group) and the results are presented as the mean ± SEM; *p < 0.05 versus control group (unpaired two-tailed Student's t-test). E) Percentage of CD4+CD25+Foxp3+ regulatory T cells in tumors was analyzed using flow cytometry at the end of treatment (n = 6, each group) and the results are presented as the mean ± SEM; *p < 0.05 versus control group (unpaired two-tailed Student's t-test). F–I) The levels of cytokines IFN γ, IL-10, and TGF-β were evaluated in tumor tissue homogenates by ELISA (n = 6, each group). *p < 0.05 and **p < 0.01 versus control group and #p < 0.05 versus DOX-treated group (unpaired two-tailed Student's t-test). La, Lacidipine.

Fig. 6. TLR4 KO affected the differentiation of Th cells in the spleen of 18 M old mice. a Relative image of the spleen and spleen index, n = 6. b Relative mRNA expression of Tbet, Gata3, Rorγt and Foxp3, n = 6. c Relative mRNA expression of important Th downstream genes in the spleen, n = 6. d Protein expression of Tbet, Gata3, Rorγt and Foxp3 and the relative Tbet, Gata3, Rorγt and Foxp3 protein levels is shown in bar graph, n = 3. e Serum levels of IL-1β, IL-6, IL-10 and TNF-α protein were measured by ELISA, n = 8. Data are presented as mean ± S.E.M.

Figure 4.| Suppressive activity of psoriatic Treg is diminished compared to heathy controls and SB restores this effect. (e) Paraffin sections were stained with antihuman Foxp3 (green) and anti-human CD25 (red) antibody. Nuclear staining was performed with DAPI (blue). Scale bar=10 µm

Fig. 7 Tissue and cellular distribution of APOE+CTSZ+TAM, GLUL+ cells, and Treg. (A, B) Multiplex immunofluorescence staining of CD68 (green), APOE (red), CTSZ (yellow), GLUL (purple), and DAPI (blue) on CRC tissue section of patient PA2203077 and patient PA2220884, scale bar 20 μm. Left: merged and single‐channel photo of the tissue section. Right: combined channel of CD68/APOE/GLUL and CD68/CTSZ/GLUL on the tissue section. (C, D) Multiplex immunofluorescence staining of CD68 (green), APOE (red), CTSZ (yellow), FOXP3 (purple), and DAPI (blue) on CRC tissue section of patient PA2203077 and patient PA2220884, scale bar 20 μm. Left: Merged and single‐channel photo of the tissue section. Right: Combined channel of CD68/APOE/FOXP3 and CD68/CTSZ/FOXP3 on the tissue section. Arrows indicate the representative regions with three immunofluorescence staining.