c-Met Antibody - #AF6128

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产品: c-Met 抗体
货号: AF6128
描述: Rabbit polyclonal antibody to c-Met
应用: WB IHC IF/ICC
文献验证: WB, IHC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
蛋白号: P08581
RRID: AB_2835011

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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说明书
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
c-Met Antibody detects endogenous levels of total c-Met.
RRID:
AB_2835011
引用格式: Affinity Biosciences Cat# AF6128, RRID:AB_2835011.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

AUTS9; c met; D249; Hepatocyte growth factor receptor; HGF; HGF receptor; HGF/SF receptor; HGFR; MET; Met proto oncogene tyrosine kinase; MET proto oncogene, receptor tyrosine kinase; Met proto-oncogene (hepatocyte growth factor receptor); Met proto-oncogene; Met protooncogene; MET_HUMAN; Oncogene MET; Par4; Proto-oncogene c-Met; RCCP2; Scatter factor receptor; SF receptor; Tyrosine-protein kinase Met;

抗原和靶标

免疫原:

A synthesized peptide derived from human c-Met, corresponding to a region within the internal amino acids.

基因/基因ID:
MET(4233)
描述:
The proto-oncogene MET product is the hepatocyte growth factor receptor and encodes tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor.

研究领域

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Adherens junction.   (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.

· Human Diseases > Infectious diseases: Bacterial > Bacterial invasion of epithelial cells.

· Human Diseases > Infectious diseases: Bacterial > Epithelial cell signaling in Helicobacter pylori infection.

· Human Diseases > Infectious diseases: Parasitic > Malaria.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Human Diseases > Cancers: Specific types > Renal cell carcinoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Melanoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Gastric cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Central carbon metabolism in cancer.   (View pathway)

· Organismal Systems > Development > Axon guidance.   (View pathway)

文献引用

1). MANF serves as a novel hepatocyte factor to promote liver regeneration after 2/3 partial hepatectomy via doubly targeting Wnt/β-catenin signaling. Cell death & disease, 2024 (PubMed: 39289348) [IF=8.1]
2). Novel Peptide CM 7 Targeted c-Met with Antitumor Activity. MOLECULES, 2020 (PubMed: 31973231) [IF=4.6]

Application: WB    Species: human    Sample: MKN-45 and MKN-28 cells

Figure 3.| The expression of c-Met in MKN-45 and MKN-28 cells was determined by flow cytometry(A,B). (C) The expression of c-Met in MKN-45 and MKN-28 cells was determined by Western blot.
3). Listeria monocytogenes: possible mechanism of infection of goat uterus and its effects on uterine autophagy and cell apoptosis. Frontiers in veterinary science, 2024 (PubMed: 39220769) [IF=3.2]

Application: WB    Species: Human    Sample: uterine tissues

Figure 2. Expression patterns of E-cadherin and c-Met proteins in uterine tissues (A1–D2). Expression levels of E-cadherin and c-Met proteins in uterine tissues (E,F). Expression levels of E-cadherin and c-Met mRNA in uterine tissues (G). The brown area indicates the location of the target protein.*p 
4). Autophagy Regulatory Genes MET and RIPK2 Play a Prognostic Role in Pancreatic Ductal Adenocarcinoma: A Bioinformatic Analysis Based on GEO and TCGA. BioMed research international, 2020 (PubMed: 33204717) [IF=2.6]

Application: IHC    Species: human    Sample:

Figure 9 (f) Protein expression pattern of MET and RIPK2. (a) Protein expression overview of MET in various normal organs. (b) Protein expression overview of MET in various cancers. (c) Protein expression overview of RIPK2 in various normal organs. (d, e) Protein expression overview of RIPK2 in various cancers. (f–h) Representative images of IHC staining of PDAC and adjacent pancreatic tissues (200x).
5). Long non‑coding RNA B3GALT5‑AS1 contributes to the progression of gastric cancer via interacting with CSNK2A1. Experimental and Therapeutic Medicine, 2021 (PubMed: 34306196) [IF=2.4]

Application: WB    Species: Human    Sample: gastric cancer cell

Figure 4 B3GALT5-AS1/CSNK2A1 axis regulates cell apoptosis. (A) Cell apoptosis in different transfection groups was assessed using a TUNEL assay. Magnification, x100. (B) Western blot analysis showing the protein levels of Bcl-2, Bax, cleaved caspase-3, caspase-3, p-c-Met and c-Met in different groups. ***P<0.001 vs. shRNA-NC; #P<0.05 and ###P<0.001 vs. shRNA-B3GALT5-AS1-1 + ov-NC. B3GALT5-AS1, β-1,3-galactosyltransferase 5-AS1; CSNK2A1, casein kinase 2 a1; p-, phosphorylated; Ov, expression; shRNA, short hairpin RNA; NC, negative control.

Application: WB    Species: human    Sample: MKN‑45 cells

Figure 4.| B3GALT5‑AS1/CSNK2A1 axis regulates cell apoptosis. (B) Western blot analysis showing the protein levels of Bcl‑2, Bax, cleaved caspase‑3, caspase‑3, p‑c‑Met and c‑Met in different groups.

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