Phospho-MLC2 (Ser19) Antibody - #AF3829

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产品: 磷酸化 MLC2 (Ser19) 抗体
货号: AF3829
描述: Rabbit polyclonal antibody to Phospho-MLC2 (Ser19)
应用: IHC IF/ICC
文献验证: IHC
反应: Human, Mouse, Rat
蛋白号: P10916
RRID: AB_2847143

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   规格 价格 库存
 100ul RMB¥ 2800 现货
 200ul RMB¥ 3800 现货

货期: 当天发货

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说明书
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Phospho-MLC2 (Ser19) Antibody detects endogenous levels of MLC2 only when phosphorylated at Ser19.
RRID:
AB_2847143
引用格式: Affinity Biosciences Cat# AF3829, RRID:AB_2847143.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Cardiac myosin light chain-2; Cardiac ventricular myosin light chain 2; CMH10; MLC 2v; MLC-2; MLC-2v; MLC2; MLRV_HUMAN; MYL 2; MYL2; Myosin light chain 2 regulatory cardiac slow; Myosin light polypeptide 2 regulatory cardiac slow; Myosin regulatory light chain 2; Myosin regulatory light chain 2 ventricular/cardiac muscle isoform; Regulatory light chain of myosin; RLC of myosin; Slow cardiac myosin regulatory light chain 2; ventricular/cardiac muscle isoform;

抗原和靶标

免疫原:

A synthesized peptide derived from human MLC2 around the phosphorylation site of Ser19.

基因/基因ID:
MYL2(4633)

研究领域

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Environmental Information Processing > Signal transduction > Apelin signaling pathway.   (View pathway)

· Human Diseases > Cardiovascular diseases > Hypertrophic cardiomyopathy (HCM).

· Human Diseases > Cardiovascular diseases > Dilated cardiomyopathy (DCM).

· Organismal Systems > Circulatory system > Cardiac muscle contraction.   (View pathway)

· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes.   (View pathway)

· Organismal Systems > Immune system > Leukocyte transendothelial migration.   (View pathway)

文献引用

1). Thrombospondin 1 Promotes Cytoskeleton Remodeling, Dedifferentiation, and Pulmonary Metastasis through ITGA1 and ITGA6 in Osteosarcoma. International journal of biological sciences, 2025 (PubMed: 40083708) [IF=8.2]

Application: IHC    Species: Mouse    Sample:

Figure 6 Knocking down of THBS1 inhibits pulmonary metastasis in vivo. (A) The scheme for establishing pulmonary metastasis model in nude mice by tail vein injection or proximal tibia injection are shown. (B) A nude mouse pulmonary metastasis model was established by injecting tumor cells into the tail vein, showing the general morphology of the lungs, H&E staining, p-MLC2 staining, Stro-1 staining and CD117 staining. (C) The number of metastatic nodules was evaluated and analyzed in osteosarcoma cells transfected with lenti-shTHBS1 and the negative control group. (D) The weights of the lungs in shCtrl and shTHBS1-1 groups were determined. (E) Analysis of expression changes of p-MLC2, Stro-1 and CD117 in metastatic nodules following THBS1 knockdown. (F) A nude mouse pulmonary metastasis model was established by injecting tumor cells into the proximal tibia, showing the in situ tumors, general morphology of the lungs, H&E staining, p-MLC2 staining, Stro-1 staining and CD117 staining. (G-J) The volume and weight of in situ tumors, as well as the number of metastatic nodules and lung weights, were determined in both the shCtrl and shTHBS1-1 groups. (K) Analysis of expression changes of p-MLC2, Stro-1 and CD117 in metastatic nodules.
2). Acupuncture Improves Synaptic Plasticity of SAMP8 Mice through the RhoA/ROCK Pathway. Current Alzheimer research, 2023 (PubMed: 37723951) [IF=1.8]
3). Research on the Protective Effect of MiR-185-3p Mediated by Huangqin-Tang Decoction (HQT) on the Epithelial Barrier Function of Ulcerative Colitis. Evidence-based Complementary and Alternative Medicine, 2021 (PubMed: 34970325)

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