Phospho-Smad7 (Ser249) Antibody - #AF3827

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产品: 磷酸化 Smad7 (Ser249) 抗体
货号: AF3827
描述: Rabbit polyclonal antibody to Phospho-Smad7 (Ser249)
应用: IHC
文献验证:
反应: Human, Mouse, Rat
蛋白号: O15105
RRID: AB_2847141

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   规格 价格 库存
 100ul RMB¥ 2800 现货
 200ul RMB¥ 3800 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Phospho-Smad7 (Ser249) Antibody detects endogenous levels of Smad7 only when phosphorylated at Ser249.
RRID:
AB_2847141
引用格式: Affinity Biosciences Cat# AF3827, RRID:AB_2847141.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CRCS3; FLJ16482; hSMAD 7; hSMAD7; MAD (mothers against decapentaplegic Drosophila) homolog 7; MAD; Mad homolog 7; MAD homolog 8; MAD mothers against decapentaplegic homolog 7; MADH 7; MADH 8; MADH6; MADH7; MADH8; Mothers Against Decapentaplegic Drosophila Homolog of 6; Mothers Against Decapentaplegic Drosophila Homolog of 7; Mothers against decapentaplegic homolog 7; Mothers against decapentaplegic homolog 8; Mothers against DPP homolog 7; Mothers against DPP homolog 8; SMA- AND MAD-RELATED PROTEIN 7; SMAD 7; SMAD; SMAD family member 7; SMAD, mothers against DPP homolog 7 (Drosophila); SMAD, mothers against DPP homolog 7; SMAD6; Smad7; SMAD7_HUMAN;

抗原和靶标

免疫原:

A synthesized peptide derived from human Smad7 around the phosphorylation site of Ser249.

基因/基因ID:
SMAD7(4092)

研究领域

· Environmental Information Processing > Signal transduction > TGF-beta signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway.   (View pathway)

文献引用

1). Mesenchymal stem cells ameliorate silica‐induced pulmonary fibrosis by inhibition of inflammation and epithelial‐mesenchymal transition. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, 2021 (PubMed: 34076355) [IF=5.3]

Application: WB    Species: Rat    Sample: lung tissues

FIGURE 5 BMSCs blocked the activation of TGF‐β/Smad pathway. (A) The mRNA expression of TGF‐β1 in lung tissue. n = 3 rats per group. (B) The protein expression levels of TGF‐β1 in lung tissues detected by ELISA. n = 7 rats per group. (C) The protein expression levels of TGF‐β1 in BALF detected by ELISA. n = 3 rats per group. (D) Western blot results of TGF‐β1, Smad2, p‐Smad2, Smad3, p‐Smad3 and Smad7 protein expression levels. n = 3 rats per group. (E‐H) The quantitative protein expression of TGF‐β1, p‐Smad2, p‐Smad3 and Smad7 in lung tissues. mRNA expression values were normalized to GAPDH. Data were presented as mean ± SD. * P <.05, ** P <.01
2). Molecular mechanism of Gan-song Yin inhibiting the proliferation of renal tubular epithelial cells by regulating miR-21-5p in adipocyte exosomes. Journal of ethnopharmacology, 2024 (PubMed: 38043753) [IF=4.8]

Application: WB    Species: Mouse    Sample: TCMK-1 cells

Fig. 4. Mechanism of GSY on TCMK-1. A. Viability of TCMK-1 cells treated with different concentrations of GSY. B. TCMK-1 cell viability in each experimental group. C. Changes in TCMK-1 cell cycle level after GSY intervention. D. Changes in apoptosis level of TCMK-1 cells after GSY intervention. E. Expression of TGF-β1, SMAD2, SMAD3, SMAD7, p-SMAD2, p-SMAD3 and p-SMAD7 proteins in TCMK-1 cells after GSY intervention. F. Gene expression levels of TGF-β1, SMAD2, SMAD3 and SMAD7 in TCMK-1 cells after GSY intervention. *p < 0.05; **p < 0.01; ***p < 0.001. NC: Negative control; MOD: Model (60 mmol/L glucose). All experiments were repeated three times.
3). A neuro-lymphatic communication guides lymphatic development by CXCL12 and CXCR4 signaling. Development (Cambridge, England), 2024 (PubMed: 39470100) [IF=4.6]
4). Cyclin-dependent kinase subunit2 (CKS2) promotes malignant phenotypes and epithelial-mesenchymal transition-like process in glioma by activating TGFβ/SMAD signaling. Cancer Medicine, 2023 (PubMed: 36284444) [IF=2.9]

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